The primary aim of the presented paper is the identification of barriers to knowledge sharing by scientific R&D project team members in a Czech academic environment. In order to fulfill this aim, secondary analysis was used to process the literature search in the context of the problem being solved, and qualitative and quantitative research in the form of a structured interview and a questionnaire survey were used to identify the barriers to knowledge sharing by scientific R&D project team members. The essential output of the presented paper is the identification of barriers to knowledge sharing by members of the scientific R&D project team in Czech public and private universities and the discussion of their causes and impacts in scientific R&D projects. With regard to the aim of the presented paper and the formulated research questions, our effort is that the presented discussion outputs are not only academic considerations but scientific analyses and outputs capable of concrete life. Above all, we propose an innovation of Riege's model of barriers to knowledge sharing, as it was chosen as a comparative basis for our research; however, it was found that it is not applicable in the current conditions of scientific R&D project management in the Czech academic environment. Doi: 10.28991/HIJ-2023-04-01-02 Full Text: PDF