The intrinsic resistance of Fusarium solani to the Fusarium-specific fungicide phenamacril is attributed to the natural variation of both T218S and K376M in myosin5

Mao, Yushuai; Zhang, Ziyang; Shen, Jinghan; Yin, Xiaoru; Wang, Tianshi; Zheng, Xuanming; Sheng, Guilin; Cai, Yiqiang; Shen, Yingchun; Chen, Yuanyuan; Zhou, Mingguo; Duan, Yabing

doi:10.1016/j.pestbp.2023.105595

Cited by 4 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amplification of these fragments was achieved through TEF-1α-F/TEF-1α-R and ITS1/ITS4 primers, respectively. 11 Czapek−Dox medium (1 g of NaNO 3 , 1 g of KH 2 PO 4 , 0.5 g of MgSO 4 •7H 2 O, 0.5 g of KCl, 0.01 g of FeSO 4 , 30 g of sucrose, and 16 g of agar in 1 L water) was utilized for the fungicide taming assay. PDA medium (200 g potato, 16 g dextrose, and 20 g agar in 1 L water) and AEA medium (5 g yeast extraction, 6 g NaNO 3 , 1.5 g KH 2 PO 4 , 0.5 g KCl, 0.25 g MgSO 4 , 16 g agar, and 20 mL glycerol in 1 L water) were employed for regular mycelial growth and fungicide sensitivity testing cultures.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Resistance Risk and Molecular Mechanism of Tomato Wilt Pathogen Fusarium oxysporum f. sp. lycopersici to Pyraclostrobin

Mao,

Qiu,

Gao

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Tomato wilt disease caused by Fusarium oxysporum f. sp. lycopersici (Fol) results in a decrease in tomato yield and quality. Pyraclostrobin, a typical quinone outside inhibitor (QoI), inhibits the cytochrome bc1 complex to block energy transfer. However, there is currently limited research on the effectiveness of pyraclostrobin against Fol. In this study, we determined the activity of pyraclostrobin against Fol and found the EC 50 values for pyraclostrobin against 100 Fol strains (which have never been exposed to QoIs before). The average EC 50 value is 0.3739 ± 0.2413 μg/mL, indicating a strong antifungal activity of pyraclostrobin against Fol, as shown by unimodal curves of the EC 50 values. Furthermore, we generated five resistant mutants through chemical taming and identified four mutants with high-level resistance due to the Cytb-G143S mutation and one mutant with medium-level resistance due to the Cytb-G137R mutation. The molecular docking results indicate that the Cytb-G143S or Cytb-G137R mutations of Fol lead to a change in the binding mode of Cytb to pyraclostrobin, resulting in a decrease in affinity. The resistant mutants exhibit reduced fitness in terms of mycelial growth (25 and 30 °C), virulence, and sporulation. Moreover, the mutants carrying the Cytb-G143S mutation suffer a more severe fitness penalty compared to those carrying the Cytb-G137R mutation. There is a positive correlation observed among azoxystrobin, picoxystrobin, fluoxastrobin, and pyraclostrobin for resistant mutants; however, no crossresistance was detected between pyraclostrobin and pydiflumetofen, prochloraz, or cyazofamid. Thus, we conclude that the potential risk of resistance development in Fol toward pyraclostrobin can be categorized as ranging from low to moderate.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the strains were identified using partial sequences of transcriptional elongation factor 1α and internal transcribed spacer fragments. The amplification of these fragments was achieved through TEF-1α-F/TEF-1α-R and ITS1/ITS4 primers, respectively …”

Section: Methodsmentioning

confidence: 99%

Resistance Risk and Molecular Mechanism of Tomato Wilt Pathogen Fusarium oxysporum f. sp. lycopersici to Pyraclostrobin

Mao,

Qiu,

Gao

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…For instance, according to Montoya-Martinez and Cota-Barreras [9,10], Fusarium languescens is a significant regional phytopathogen in Sonora, Mexico, that could impact potato production. Currently, new alternative control methods to synthetic fungicides are being considered for sustainable agricultural systems, due to the development of fungicide resistance by Fusarium strains [11][12][13], as well as the negative impact of these synthetic compounds on the environment and health [14]. Thus, a promising approach that has gained attention is the use of biological control agents (BCAs) [15,16].…”

Section: Introductionmentioning

confidence: 99%

Genomic Insight into a Potential Biological Control Agent for Fusarium-Related Diseases in Potatoes: Bacillus cabrialesii Subsp. cabrialesii Strain PE1

Valenzuela-Aragon,

Montoya-Martínez,

Parra-Cota

et al. 2024

Horticulturae

View full text Add to dashboard Cite

Bacillus strain PE1, which was isolated from potatoes harvested in the Yaqui Valley, Mexico, was evaluated as a potential biological control agent against Fusarium languescens. The draft genome sequence was obtained through Illumina NovaSeq sequencing, revealing a genomic size of 4,071,293 bp, with a G + C content of 44.13%, an N50 value of 357,305 bp, and 27 contigs. The taxonomic affiliation was confirmed by analyzing the 16S rRNA gene and overall genome relatedness indices (OGRIs) and constructing a phylogenomic tree based on the whole genome, which showed a close relationship to Bacillus cabrialesii subsp. cabrialesii. Genomic annotation using RAST and Prokka identified 4261 coding DNA sequences (CDSs) distributed across 331 subsystems, highlighting genes associated with biocontrol, stress response, and iron acquisition. AntiSMASH 7.1 was used for genome mining, revealing seven biosynthetic gene clusters that potentially produce biocontrol-related metabolites. In vitro assays confirmed the antagonistic activity of strain PE1 against Fusarium languescens CE2, demonstrating its potential to inhibit mycelial growth. The study provides a genomic basis for investigating B. cabrialesii subsp. cabrialesii PE1 as a potential biological control agent in potato production.

show abstract

The G143S mutation in cytochrome b confers high resistance to pyraclostrobin in Fusarium pseudograminearum

Zhang,

Li,

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDWheat crown rot (WCR), primarily caused by Fusarium pseudograminearum has become more and more prevalent in winter wheat areas in China. However, limited fungicides have been registered for the control of WCR in China so far. Pyraclostrobin is a representative quinone outside inhibitor (QoI) with excellent activity against Fusarium spp. There is currently limited research on the resistance risk and resistance mechanism of F. pseudograminearum to pyraclostrobin.RESULTSHere, we determined the activity of pyraclostrobin against F. pseudograminearum. The EC50 values ranged from 0.022 to 0.172 μg mL−1 with an average EC50 value of 0.071 ± 0.030 μg mL−1. Four highly pyraclostrobin‐resistant mutants were obtained from two sensitive strains by ultraviolet (UV) mutagenesis in the laboratory. The mutants showed decreased mycelial growth rate and virulence as compared with the corresponding wild‐type strains, indicating that pyraclostrobin resistance suffered a fitness penalty in F. pseudograminearum. It was found that the high resistance of four mutants was caused by the G143S mutation in Cytb. Molecular docking analysis also further confirms that the G143S mutation in Cytb decreased the binding affinity between pyraclostrobin and Cytb.CONCLUSIONThe resistance risk of F. pseudograminearum to pyraclostrobin could be low to medium. Although a mutation at the G143S position of Cytb could potentially occur, this mutation decreases the fitness of the mutant, which may reduce its survival in the environment. Therefore, the negative consequences of a possible mutation are lower. This makes pyraclostrobin a good candidate for controlling crown rot in wheat. © 2024 Society of Chemical Industry.

show abstract

The intrinsic resistance of Fusarium solani to the Fusarium-specific fungicide phenamacril is attributed to the natural variation of both T218S and K376M in myosin5

Cited by 4 publications

References 43 publications

Resistance Risk and Molecular Mechanism of Tomato Wilt Pathogen Fusarium oxysporum f. sp. lycopersici to Pyraclostrobin

Resistance Risk and Molecular Mechanism of Tomato Wilt Pathogen Fusarium oxysporum f. sp. lycopersici to Pyraclostrobin

Genomic Insight into a Potential Biological Control Agent for Fusarium-Related Diseases in Potatoes: Bacillus cabrialesii Subsp. cabrialesii Strain PE1

The G143S mutation in cytochrome b confers high resistance to pyraclostrobin in Fusarium pseudograminearum

Contact Info

Product

Resources

About