The investigation of anti-inflammatory activity of volatile oil of Angelica sinensis by plasma metabolomics approach

Yao, Wan-Ling; Zhang, Ling; Hua, Yuejin; Ji, Peng; Li, Pengling; Li, Jinxia; Zhong, Lijia; Zhao, Haifu; Wei, Yanming

doi:10.1016/j.intimp.2015.11.006

Cited by 45 publications

(25 citation statements)

References 39 publications

(43 reference statements)

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“…Pharmacological studies have indicated that the VOAS has evident anti-inflammatory, neuroprotective, immunomodulatory and anti-cardiovascular activities (Wedge et al, 2009;Chao et al, 2011). In addition, some results from our previous studies have shown that the VOAS inhibits the production of inflammatory mediators and alleviates acute inflammatory damage Yao et al, 2015;Hua et al, 2015).Therefore, the anti-inflammatory action of VOAS was chosen as the subjects of this study, based on the traditional use of AS in Pharmacopoeia and the results of the clinical investigations.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the anti-inflammatory effects of volatile oils from processed products of Angelica sinensis radix by GC–MS-based metabolomics

Zhong

Hua

et al. 2016

Journal of Ethnopharmacology

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Section: Introductionmentioning

confidence: 99%

Evaluation of the anti-inflammatory effects of volatile oils from processed products of Angelica sinensis radix by GC–MS-based metabolomics

Zhong

Hua

et al. 2016

Journal of Ethnopharmacology

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“…Excessive accumulation of BLA is a factor causing fatigue, which can induce a decrease in pH that inhibits muscle contraction. BUN is the metabolic outcome of proteins and amino acids, which undergo a strong catabolism when the organism cannot gain enough energy from sugar and fats (Zhao et al, ). There are positive correlations between the levels of BLA, BUN and exercise endurance.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we have used GC/MS‐based metabonomics to elucidate metabolic mechanisms in fatigued mice after the intervention of YGJ and identified crucial biomarkers (Wang et al, ). The application of the metabolomic approach based on GC/MS has proved to be of great value in the evaluation of the therapeutic effects and elucidation of the therapeutic mechanisms of a series of traditional Chinese medicines, such as volatile oil of A. sinensis (Yao et al, ), Xiaoyaosan (Tian et al, ) and Quzhuotongbi (Chen et al, ).…”

Section: Introductionmentioning

confidence: 99%

Metabonomic analysis of serum reveals antifatigue effects of Yi Guan Jian on fatigue mice using gas chromatography coupled to mass spectrometry

Shui

Cai

Huang

et al. 2017

Biomedical Chromatography

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Yi Guan Jian (YGJ), one of the most commonly used traditional Chinese medicines, has been reported to possess significant antifatigue effects. However, the mechanisms underlying its antifatigue effects remain largely unresolved. In this study, a metabonomics approach, involving gas chromatography coupled to mass spectrometry and a multivariate statistical technique, was developed to estimate the extent to which YGJ alleviated the exhausting swimming-induced fatigue of mice. High-dose treatment with YGJ significantly extended the swimming time of fatigued mice. Significant alterations of metabolites involving amino acids, organic acids and carbohydrates were observed in the serum of fatigued mice, which were reversed by YGJ treatment while biochemical indexes returned to normal. These metabolic changes suggest that the antifatigue effect of YGJ is associated with the impairement of amino acid, organic acids and carbohydrates. It also appears that YGJ can induce significant metabolic alterations independent of the exhausting swimming-induced metabolic changes. The significantly altered metabolites induced by YGJ intervention include l-2-amino-acetoacetate, taurine, fumaric acid, malic acid, oxoadipic acid and l-aspartate, all of which are associated with antifatigue properties. This suggests that YGJ exerts chemopreventive effects via antifatigue mechanisms.

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“…The supernatant (300 l) was transferred to the GC vial and evaporated until it was dry. Chemical derivatization of sample metabolites was performed using a 100-l aliquot of methoxyamine pyridine solution (15 mg/ml) at 70°C for 1 h. Subsequently, N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (50 l; Sigma-Aldrich) was added, and the silylation was performed at 70°C for 1 h. After adding 150 l of n-heptane and vortex mixing, the supernatant was transferred to the GC microvial for GC-MS analysis (44,45).…”

Section: Methodsmentioning

confidence: 99%

Elucidating the Role and Regulation of a Lactate Permease as Lactate Transporter in Bacillus coagulans DSM1

Wang

Zhang

Liu

et al. 2019

Appl Environ Microbiol

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A key feature of Bacillus coagulans is its ability to produce L-lactate via homofermentative metabolism. A putative lactate permease-encoding gene (lutP) and the gene encoding its regulator (lutR) were identified in one operon in B. coagulans strains. LutP orthologs are highly conserved and located adjacent to the gene cluster related to lactate utilization in most lactate-utilizing microorganisms. However, no lactate utilization genes were found adjacent to lutP in all sequenced B. coagulans strains. The stand-alone presence of lutP in L-lactate producers indicates that it may have functions in lactate production. In this study, B. coagulans DSM1 was used as a representative strain, and the critical roles of LutP and its regulation were described. Transport property assays showed that LutP was essential for lactate uptake. Its regulator LutR directly interacted with the lutP-lutR intergenic region, and lutP transcription was activated by L-lactate via regulation by LutR. A biolayer interferometry assay further confirmed that LutR bound to an 11-bp inverted repeat in the intergenic region, and lutP transcription began when the binding of LutR to the lutP upstream sequence was inhibited. We conclusively showed that lutP encodes a functional lactate permease in B. coagulans. IMPORTANCE Lactate-utilizing strains require lactate permease (LutP) to transport lactate into cells. Bacillus coagulans LutP is a previously uncharacterized lactate permease with no lactate utilization genes situated either adjacent to or remotely from it. In this study, an active lactate permease in an L-lactate producer, B. coagulans DSM1, was identified. Lactate supplementation regulated the expression of lactate permease. This study presents physiological evidence of the presence of a lactate transporter in B. coagulans. Our findings indicate a potential target for the engineering of strains in order to improve their fermentation characteristics.

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The investigation of anti-inflammatory activity of volatile oil of Angelica sinensis by plasma metabolomics approach

Cited by 45 publications

References 39 publications

Evaluation of the anti-inflammatory effects of volatile oils from processed products of Angelica sinensis radix by GC–MS-based metabolomics

Evaluation of the anti-inflammatory effects of volatile oils from processed products of Angelica sinensis radix by GC–MS-based metabolomics

Metabonomic analysis of serum reveals antifatigue effects of Yi Guan Jian on fatigue mice using gas chromatography coupled to mass spectrometry

Elucidating the Role and Regulation of a Lactate Permease as Lactate Transporter in Bacillus coagulans DSM1

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