The Involvement of Copper Transporter in Lead-induced Oxidative Stress in Astroglia

Qian, Yongchang; Zheng, Ying; Ramos, Kenneth S.; Tiffany‐Castiglioni, Evelyn

doi:10.1007/s11064-005-2677-1

Cited by 37 publications

(32 citation statements)

References 40 publications

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“…ATP7A is known to bind other metals such as silver (Cobine et al, 2000), and the metallochaperone Atx1 has been shown to deliver cadmium and mercury to CCC2, the ATP7B equivalent in yeast (Morin et al, 2004). It has also been reported recently that ATP7A is capable of conferring resistance to lead in astroglia in a manner that required the metal binding domains of this protein (Qian et al, 2005). ATP7B is found in the membranes of secretory vesicles, and it can clearly mediate resistance to DDP and enhance its intracellular sequestration (Safaei, 2006).…”

Section: Discussionmentioning

confidence: 99%

Transport of Cisplatin by the Copper Efflux Transporter ATP7B

Safaei

Otani²,

Larson³

et al. 2007

Mol Pharmacol

100

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ATP7B is a P-type ATPase that mediates the efflux of copper. Recent studies have demonstrated that ATP7B regulates the cellular efflux of cisplatin (DDP) and controls sensitivity to the cytotoxic effects of this drug. To determine whether DDP is a substrate for ATP7B, DDP transport was assayed in vesicles isolated from Sf9 cells infected with a baculovirus that expressed either the wild-type ATP7B or a mutant ATP7B that was unable to transport copper as a result of conversion of the transmembrane metal binding CPC motif to CPA. Only the wild-type ATP7B-expressing vesicles exhibited copper-dependent ATPase activity, copper-induced acyl-phosphate formation, and ATP-dependent transport of copper. The amount of DDP that became bound was higher for vesicles expressing either type of ATP7B than for those not expressing either form of ATP7B, but only the vesicles expressing wild-type ATP7B mediated ATP-dependent accumulation of the drug. At pH 4.6, the vesicles expressing the wild-type ATP7B exhibited ATPdependent accumulation of DDP with an apparent K m of 1.2 Ϯ 0.5 (S.E.M.) M and V max of 0.03 Ϯ 0.002 (S.E.M.) nmol/mg of protein/min. DDP also induced the acyl-phosphorylation of ATP7B but at a much slower rate than copper. Copper and DDP each inhibited the ATP-dependent transport of the other. These results establish that DDP is a substrate for ATP7B but is transported at a much slower rate than copper.

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Section: Discussionmentioning

confidence: 99%

Transport of Cisplatin by the Copper Efflux Transporter ATP7B

Safaei

Otani²,

Larson³

et al. 2007

Mol Pharmacol

100

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“…Astrocyte cultures were established using a modification of methods described by (Guilian and Baker, 1986) and (Lehnardt et al, 2002), expert advice from Dr. E. Tiffany-Castiglioni (Lindahl et al, 1999;Qian et al, 2005) and a previously established laboratory protocol (Johnson and Sohrabji, 2005). The experimental protocol is similar to those used in other published studies using astrocyte cultures .…”

Section: Tissue Culture -Astrocytesmentioning

confidence: 99%

Effects of estrogen receptor agonists on regulation of the inflammatory response in astrocytes from young adult and middle-aged female rats

Lewis¹,

Johnson²,

Stohlgren³

et al. 2008

Journal of Neuroimmunology

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Estrogen has been shown to attenuate the inflammatory response following injury or lipopolysaccharide treatment in several organ systems. Estrogen's actions are transduced through two estrogen receptor sub-types, estrogen receptor (ER) -alpha and estrogen receptor-beta, whose actions may be overlapping or independent of each other. The present study examined the effects of ERα-and ERβ-specific ligands in regulating the inflammatory response in primary astrocyte cultures. Pre-treatment with 17β-estradiol (ERα/ERβ agonist), HPTE (ERα agonist/ERβ antagonist) and DPN (ERβ agonist) led to attenuation of IL-1β, TNFα, and MMP-9 in astrocyte media derived from young adult (3-4 mos.) and reproductive senescent female (9-11 mos., acyclic) astrocyte cultures, while pretreatment with PPT (ERα agonist) attenuated IL-1β (but not TNFα or MMP-9) in both young and senescent-derived astrocyte cultures. Our previous work determined that 17β-estradiol was unable to attenuate the LPS-induced increase in IL-1β in olfactory bulb primary microglial cultures derived from either young adult or reproductive senescent females. In young adult-derived microglial cultures, the LPS-induced increase in IL-1β was not attenuated by pre-treatment with 17β-estradiol, PPT or HPTE. Interestingly, the ERβ agonist, DPN significantly decreased IL-1β following LPS treatment in young adult-derived microglia. Thus while both microglia and astrocytes synthesize and release inflammatory mediators, the present data shows that compounds which bind ERβ are more effective in attenuating proinflammatory cytokines in both cell types and may therefore be a more effective agent for future therapeutic use.

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“…This may suggesting that N application would result in more biomass production by increasing the photosynthetic rate (Landberg and Greger 2003) It have been reported that heavy metal stress induced by Cd generate Reactive Oxygen Species (ROS) production in plants which lead to different damages. Lipid peroxydation is considered as the most damaging process known to occur due to ROS production (Qian et al, 2005). Our results showed that TBARS content is two times higher in shoots than in roots.…”

Section: Discussionmentioning

confidence: 46%

“…Antioxidants play an important role in adaptation and ultimate survival of plants under heavy metal stress conditions (Qian et al, 2005). One of the protective mechanisms is the enzymatic antioxidant system, which involves catalase (CAT), an enzyme which catalyses hydrogen peroxide (H 2 O 2 ) dismutation into oxygen(O 2 ) and water (H 2 O) (Sun and Zhou 2008) Our results indicate that CAT activity significantly increased in shoots of plants exposed to Cd combined with PK fertilization.…”

Section: Discussionmentioning

confidence: 74%

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Influence of nitrate fertilization on Cd uptake and oxidative stress parameters in alfalfa plants cultivated in presence of Cd

Hattab

Boussetta

Bannı

2014

J. Soil Sci. Plant Nutr.

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Plant nutrients might affect the activity and bioavailability of heavy metals in the soil -plant environment thus their accumulation in the plant. Little is known about the effect of nitrogen fertilization on cadmium (Cd) uptake by alfalfa "Medicago sativa". This work aims to characterize the oxidative status, the physiological stress parameters and the Cd uptake in alfalfa « Medicago sativa » exposed to Cd and supplied with nitrogen fertilizers. The experiment was carried out in a green house with alfalfa grown in Cd-polluted soil (3.6 ppm) and amended with two different fertilizers: without NO 3 -(PK) or with NO 3 -(NPK) in a sand:peat (v:v) mixture. The following parameters were monitored: Chlorophyll content, Photosynthesis rate, Catalase (CAT) activity, Thiobarbutiric Acid Reactive Species (TBARS) content and Cd bioaccumulation. It was found that NPK fertilization increased mean Cd uptake as well as plant biomass in Cd-exposed plants. Nitrogen supply was also effective in reduction Cd-induces phytotoxicity (Photosynthetic pigments and rate) and oxidative stress alterations. Our results suggest that nitrogen supply may improve the uptake rate of Cd by alfalfa and provide new insights on the importance of nitrogen fertilization towards future phytoremediation applications using alfalfa.

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The Involvement of Copper Transporter in Lead-induced Oxidative Stress in Astroglia

Cited by 37 publications

References 40 publications

Transport of Cisplatin by the Copper Efflux Transporter ATP7B

Transport of Cisplatin by the Copper Efflux Transporter ATP7B

Effects of estrogen receptor agonists on regulation of the inflammatory response in astrocytes from young adult and middle-aged female rats

Influence of nitrate fertilization on Cd uptake and oxidative stress parameters in alfalfa plants cultivated in presence of Cd

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