The involvement of hypoxia inducible factor-1α on the proportion of three types of haemocytes in Chinese mitten crab under hypoxia stress

Wang, Fengchi; Yang, Zhichao; Li, Jiaming; Ma, Yuhan; Tu, Yuhan; Zeng, Xiaorui; Wang, Qingyao; Jiang, Yusheng; Huang, Shu; Yi, Qingfeng

doi:10.1016/j.dci.2022.104598

Cited by 6 publications

(1 citation statement)

References 78 publications

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“…genscript.com/tools/real-time-pcr-taqman-primer-design-tool) (accessed on 13 December 2022). Additionally, in the study, Esβ-actin acted as an internal control [46,[51][52][53]. The specificity of primers was evaluated by 1% agarose gel electrophoresis and melting curve analysis.…”

Section: Rna Isolation Cdna Synthesis and Quantitative Real-time Pcr ...mentioning

confidence: 99%

FOXO-like Gene Is Involved in the Regulation of 20E Pathway through mTOR in Eriocheir sinensis

Yang

et al. 2023

JMSE

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The Forkhead Box O (FOXO) gene plays a key role in various biological processes, such as growth, metabolism, development, immunity and longevity. Molting is an essential process for crustacean growth, which is mainly regulated by 20-hydroxyecdysone (20E) and molt-inhibiting hormone (MIH). Although the role of FOXO in regulating the immune response of crustaceans is well documented, its involvement in controlling crustacean molting remains unclear. In this study, a FOXO-like gene (designed as EsFOXO-like) was identified in Eriocheir sinensis, and the regulation of the 20E pathway by EsFOXO-like was also investigated. The coding sequence of EsFOXO-like was 852 bp, which consisted of 283 amino acids including a conserved Forkhead (FH) domain. EsFOXO-like shared high similarity with FOXO genes from other crustaceans, and the mRNA expression levels of the EsFOXO-like gene were highest in the hepatopancreas and lowest in the hemocytes. However, transcription and protein expression of the EsFOXO-like gene were found to be up-regulated only during the pre-molt stage in the hepatopancreas, with lower expression levels observed at the post-molt stage. To explore the role of EsFOXO-like in the 20E pathway, EsFOXO-like was firstly inhibited by a specific FOXO inhibitor (AS1842856) and then through an EsFOXO-like dsRNA injection, respectively, and the results showed that the relative expression levels of EsFOXO-like were notably decreased in the hepatopancreas after both the inhibitor and dsRNA treatments. The 20E concentration, the mRNA expression levels of the 20E receptors including the ecdysone receptor (EcR) and the retinoid-X receptor (RXR) and EsmTOR transcription in the AS1842856 group or the EsFOXO-RNAi group were all significantly higher than that in the control group, while the mRNA expression level of EsMIH was significantly decreased after EsFOXO-like inhibition. To further investigate whether the EsFOXO-like acts through mTOR or not, Rapamycin was administered to inhibit mTOR activity in EsFOXO-like inhibited crabs. The results revealed a significant reduction in the concentration of 20E and the expression level of EsMIH in the AS1842856 + Rapamycin group compared to the AS1842856 + DMSO group, accompanied by an increase in EsEcR and EsRXR expression. These findings collectively suggest that EsFOXO-like regulates the 20E pathway through mTOR, which offered valuable insights into the understanding of the molting process in crustaceans.

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Section: Rna Isolation Cdna Synthesis and Quantitative Real-time Pcr ...mentioning

confidence: 99%

FOXO-like Gene Is Involved in the Regulation of 20E Pathway through mTOR in Eriocheir sinensis

Yang

et al. 2023

JMSE

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Characterization of the effects of Chinese peony (Radix paeoniae Alba) and its active ingredients on the hematopoietic function of the Chinese mitten crab (Eriocheir sinensis)

Lyu,

Shen,

Guo

et al. 2024

Aquaculture

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Comprehensive whole transcriptome analysis reveals specific lncRNA- and circRNA-ceRNA networks of Chinese mitten crab (Eriocheir sinensis) in response to hypoxia stress

Zhou,

Zhao,

Shi

et al. 2025

Aquaculture

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The involvement of hypoxia inducible factor-1α on the proportion of three types of haemocytes in Chinese mitten crab under hypoxia stress

Cited by 6 publications

References 78 publications

FOXO-like Gene Is Involved in the Regulation of 20E Pathway through mTOR in Eriocheir sinensis

FOXO-like Gene Is Involved in the Regulation of 20E Pathway through mTOR in Eriocheir sinensis

Characterization of the effects of Chinese peony (Radix paeoniae Alba) and its active ingredients on the hematopoietic function of the Chinese mitten crab (Eriocheir sinensis)

Comprehensive whole transcriptome analysis reveals specific lncRNA- and circRNA-ceRNA networks of Chinese mitten crab (Eriocheir sinensis) in response to hypoxia stress

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