The involvement of the 67kDa laminin receptor-mediated modulation of cytoskeleton in the degranulation inhibition induced by epigallocatechin-3-O-gallate

Fujimura, Yoshinori; Umeda, Daisuke; Kiyohara, Yuko; Sunada, Yousuke; Yamada, Koji; Tachibana, Hirofumi

doi:10.1016/j.bbrc.2006.07.086

Cited by 64 publications

(53 citation statements)

References 42 publications

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“…Polyphenolic antioxidants such as EGCG have been shown to possess cancer chemopreventative activity, mediated by several mechanisms that are postulated to stem either from its antioxidant effects as a ROS (reactive oxygen species) scavenger [6] or by direct inhibition of molecular targets [47][48][49]. The antioxidant activity of EGCG has been shown to regulate multiple signal transduction pathways [43,50] including the activities of several oxidative stress-responsive transcription factors such as NF-κB, AP-1 and Nrf2.…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

The green tea component EGCG inhibits RNA polymerase III transcription

Jacob

Cabarcas

Veras

et al. 2007

Biochemical and Biophysical Research Communications

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RNA polymerase III (RNA pol III) transcribes many small structural RNA molecules involved in RNA processing and translation, and thus regulates the growth rate of a cell. Accurate initiation by RNA pol III requires the initiation factor TFIIIB. TFIIIB has been demonstrated to be regulated by tumor suppressors, including ARF, p53, RB, and the RB-related pocket proteins, and is a target of the oncogene c-myc and the mitogen-activated protein kinase ERK. EGCG has been demonstrated to inhibit the growth of a variety of cancer cells, induce apoptosis and regulate the expression of p53, myc, and ERK. Thus, we hypothesized that EGCG may regulate RNA pol III transcription in cells. Here, we report that EGCG (1) inhibits RNA pol III transcription from gene internal and gene external promoters (2) EGCG inhibits protein expression of the TFIIIB subunits Brf1 and Brf2, and (3) EGCG inhibits Brf2 promoter activity in cervical carcinoma cells. KeywordsRNA polymerase III; TFIIIB; Brf1; Brf2; EGCG Catechins are flavonoids found in many plant-derived food products including fruits, berries, chocolate, wine, and green tea (Camellia sinensis) [1]. (−)-Epigallocatechin gallate (EGCG) (Figure 1) constitutes the largest percentage of catechins found in green tea, accounting for 65% of the total catechin content [2]. Epidemiological and animal studies have demonstrated that EGCG provides protection against a variety of cancers including those of the skin, lung, prostate and breast [3][4][5][6]. EGCG also possess anti-proteolytic [7,8], anti-mutagenic [9,10], and anti-proliferative [5,[11][12][13] activity, thereby regulating the growth of a cell.RNA polymerase III (RNA pol III) is the largest of the eukaryotic DNA dependent RNA polymerases, with 17 subunits, and transcribes many of the genes involved in processing (U6 snRNA) and translation (tRNA), thereby dictating the growth rate of a cell [14]. RNA pol III activity has been shown to be deregulated in a variety of cancers, reviewed in [15-© 2010 Elsevier Inc. All rights reserved 3 Corresponding author: Laura Schramm, Department of Biological Sciences, St. John's University, 8000 Utopia Parkway, Queens, NY 11439; schrammL@stjohns.edu; Fax: 718-990-5958. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Deregulation of TFIIIB-mediated transcription could be an important step in tumor development. Hence, we speculate that TFIIIB-mediated transcription may be a novel target of regulation by chemopreventive agents. NIH Public AccessEGCG and RNA polymerase III have both been demonstrated to regulate cell growth and proliferation. We hypothesi...

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Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

The green tea component EGCG inhibits RNA polymerase III transcription

Jacob

Cabarcas

Veras

et al. 2007

Biochemical and Biophysical Research Communications

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“…Furthermore, this EGCG-induced actin remodeling was abolished in both anti-67LR antibody-treated cells and 67LR-knockdowned cells. 55) Our findings indicated that EGCG-induced actin remodeling is caused by lowering MRLC phosphorylation mediated through the binding of EGCG to the 67LR (Fig. 2).…”

Section: Iiii Anti-allergic Action Of Egcg Through the 67lrmentioning

confidence: 58%

“…[52][53][54] We found that EGCG inhibited the calcium ionophore A23187-induced histamine release from human basophilic KU812 cells and did not inhibit the increase of the intracellular Ca 2+ level after stimulation with A23187. 55) This result suggested that the effect of EGCG on histamine release occurs after the elevation of the intracellular Ca 2+ concentration. Thr18/Ser19 phosphorylation of the myosin regulatory light chain (MRLC) has been reported to be temporally correlated with degranulation in rat basophilic RBL-2H3 cells, and the inhibition of MRLC phosphorylation has been shown to impair the degranulation.…”

Section: Iiii Anti-allergic Action Of Egcg Through the 67lrmentioning

confidence: 93%

“…56) Although the non-gallated type of catechin, epigallocatechin, with no ability to inhibit histamine release, showed no inhibitory effect toward MRLC phosphorylation, the gallated type of catechin, EGCG, clearly reduced the level of phosphorylated MRLC. 55) After treatment of KU812 cells with anti-67LR antibody, cells were incubated with EGCG and further challenged with A23187. The reductive effect of EGCG on the histamine release was almost completely inhibited in cells treated with the anti-67LR antibody.…”

Section: Iiii Anti-allergic Action Of Egcg Through the 67lrmentioning

confidence: 99%

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Small molecule-sensing strategy and techniques for understanding the functionality of green tea

Fujimura

2015

Bioscience, Biotechnology, and Biochemistry

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“…EGCG alters the lipid organization of the plasma membrane, causes the rearrangement of lipid rafts [83] preventing the activation of cell surface receptors. In multiple myeloma cells EGCG promoted apoptosis through lipid rafts clustering, that in turn mediated the activation of 67-LR and triggered a pro-death signaling [84][85][86][87]. GTCs inhibited RTKs activation through lipid rafts clustering.…”

Section: Cell Surface Receptorsmentioning

confidence: 97%

Green Tea Catechins for Prostate Cancer Prevention: Present Achievements and Future Challenges

Naponelli¹,

Ramazzina²,

Lenzi³

et al. 2017

Preprint

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Green Tea Catechins (GTCs) are a family of chemically related compounds usually classified as antioxidant molecules. Epidemiological evidences, supported by interventional studies, highlighted a more than promising role for GTCs in human Prostate Cancer (PCa) chemoprevention.In the last decades many efforts have been made to gain new insights into the mechanism of action of GTCs. Now it is clear that GTCs anticancer action can no longer be simplistically limited to their direct antioxidant/pro-oxidant properties. Recent contributions to the advancement of knowledge in this field have shown that GTCs specifically interact with cellular targets including, cell surface receptors, lipid rafts and endoplasmic reticulum, modulate gene expression through direct effect on transcription factors or indirect epigenetic mechanisms, interfere with intracellular proteostasis at various levels. Many of the effects observed in vitro are dose and cell context dependent and take place at concentration that cannot be achieved in vivo.Poor intestinal absorption together with an extensive systemic and enteric metabolism influence GTCs bioavailability through still poor understood mechanisms. Recent efforts to develop delivery systems that increase GTCs overall bioavailability, by mean of biopolymeric nanoparticles, represent the main way to translate preclinical results in a real clinical scenario for PCa chemoprevention.

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The involvement of the 67kDa laminin receptor-mediated modulation of cytoskeleton in the degranulation inhibition induced by epigallocatechin-3-O-gallate

Cited by 64 publications

References 42 publications

The green tea component EGCG inhibits RNA polymerase III transcription

The green tea component EGCG inhibits RNA polymerase III transcription

Small molecule-sensing strategy and techniques for understanding the functionality of green tea

Green Tea Catechins for Prostate Cancer Prevention: Present Achievements and Future Challenges

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