The Isoelectric Focusing of Creatine Kinase Variants: II. The Heterogeneity of Creatine Kinase in Human Serum with Normal and Elevated Catalytic Concentrations

Abstract

Summary: An effective and reliable method for the quantitative estimation of creatine kinase-MB, creatine kinase-MM variants and mitochondrial forms of creatine kinase in serum is presented.The high resölving power of isoelectric focusing allows the use of tetrazolium salts and meldola blue for the quantitative measfurement without interfering non-specific reduction.