The isoflavone genistein enhances osteoblastogenesis: signaling pathways involved

Cepeda, S.; Sandoval, Marisa Julia; Crescitelli, María Carla; Rauschemberger, María Belén; Massheimer, Virginia

doi:10.1007/s13105-019-00722-3

Cited by 24 publications

(22 citation statements)

References 32 publications

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“…In addition, estrogen contributes to osteogenesis and bone formation [10]. Recently, phytoestrogens were reported to possess the potential to replace estrogen for treating bone disorders [22][23][24]31]. The present study showed the valuable actions of MPP for evaluating the effects of estrogen or phytoestrogens on osteoblast maturation, bone formation, and fracture healing.…”

Section: Discussionmentioning

confidence: 51%

Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism

et al. 2020

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An estrogen deficiency is the main cause of osteoporosis in postmenopausal women. In bone remodeling, estrogen receptors (ERs) can mediate estrogen-transducing signals. Methylpiperidinopyrazole (MPP) is a highly specific antagonist of ER-alpha (ERα). This study was designed to evaluate the effects of MPP on estrogen-induced energy production, subsequent osteoblast maturation, and the possible mechanisms. Exposure of primary osteoblasts isolated from neonatal rat calvarias to MPP did not affect cell morphology or survival. Estradiol can induce translocation of ERα into mitochondria from the cytoplasm. Interestingly, pretreatment of rat calvarial osteoblasts with MPP lowered estrogen-induced ERα translocation. Sequentially, estrogen-triggered expressions of mitochondrial energy production-linked cytochrome c oxidase (COX) I and COX II messenger (m)RNAs were inhibited following pretreatment with MPP. Consequently, MPP caused decreases in estrogen-triggered augmentation of the activities of mitochondrial respiratory complex enzymes and levels of cellular adenosine phosphate (ATP). During progression of osteoblast maturation, estrogen induced bone morphogenetic protein (BMP)-6 and type I collagen mRNA expressions, but MPP treatment inhibited such induction. Consequently, estrogen-induced osteoblast activation and mineralization were attenuated after exposure to MPP. Taken together, MPP suppressed estrogen-induced osteoblast maturation through decreasing chromosomal osteogenesis-related BMP-6 and type I collagen mRNA expressions and mitochondrial ATP synthesis due to inhibiting energy production-linked COX I and II mRNA expressions. MPP can appropriately be applied to evaluate estrogen-involved bioenergetics and osteoblast maturation.

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Section: Discussionmentioning

confidence: 51%

Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism

et al. 2020

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“…In vivo, genistein attenuated cartilage degradation in two different OA animal models [58,59]. Furthermore, a positive effect on bone was obtained through enhanced osteoblastic differentiation and maturation via the activation of ER (estrogen receptor), p38 MAPK-Runx2, and NO/cGMP pathways [60][61][62]. It also inhibited osteoclast formation and bone resorption by inducing the osteoclastogenic inhibitor osteoprotegerin (OPG) and by blocking NF-κB signaling [60,63].…”

Section: Genisteinmentioning

confidence: 99%

Emerging Natural-Product-Based Treatments for the Management of Osteoarthritis

et al. 2021

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Osteoarthritis (OA) is a complex degenerative disease in which joint homeostasis is disrupted, leading to synovial inflammation, cartilage degradation, subchondral bone remodeling, and resulting in pain and joint disability. Yet, the development of new treatment strategies to restore the equilibrium of the osteoarthritic joint remains a challenge. Numerous studies have revealed that dietary components and/or natural products have anti-inflammatory, antioxidant, anti-bone-resorption, and anabolic potential and have received much attention toward the development of new therapeutic strategies for OA treatment. In the present review, we provide an overview of current and emerging natural-product-based research treatments for OA management by drawing attention to experimental, pre-clinical, and clinical models. Herein, we review current and emerging natural-product-based research treatments for OA management.

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“…It lowers the osteoclast number in neonatal bone marrow cell culture through decreasing the survival of osteoclasts or attenuating their formation ( 41 ). It also increases the differentiation and migration of primary rat calvarial osteoblasts by estrogen receptor (ER) and nitric oxide synthase pathway ( 42 ). Mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) transduction systems are shown to involve in the pro-osteogenesis effects of GEN ( 42 ).…”

Section: Mechanism Of Action Of Isoflavones On Bone Developmentmentioning

confidence: 99%

“…It also increases the differentiation and migration of primary rat calvarial osteoblasts by estrogen receptor (ER) and nitric oxide synthase pathway ( 42 ). Mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) transduction systems are shown to involve in the pro-osteogenesis effects of GEN ( 42 ). Surprisingly, in a coculture of osteoblasts and monocytes, GEN increased the formation of osteoclast-like cells ( 42 ), probably through stimulating factors from osteoblasts.…”

Section: Mechanism Of Action Of Isoflavones On Bone Developmentmentioning

confidence: 99%

“…Mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) transduction systems are shown to involve in the pro-osteogenesis effects of GEN ( 42 ). Surprisingly, in a coculture of osteoblasts and monocytes, GEN increased the formation of osteoclast-like cells ( 42 ), probably through stimulating factors from osteoblasts. Another study suggested the upregulation of Ereg and Efcab2 , as well as downregulation of Hrc, Gli , and Ifim5 in MC3T3-E1 preosteoblastic cells ( 43 ).…”

Section: Mechanism Of Action Of Isoflavones On Bone Developmentmentioning

confidence: 99%

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Skeletal Effects of Early-Life Exposure to Soy Isoflavones—A Review of Evidence From Rodent Models

Chin

Pang

2020

Front. Pediatr.

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Isoflavones are dietary phytoestrogens commonly found in soy-based products. The widespread presence of isoflavones in soy infant formula and breast milk may have long-lasting effects on the development of sex hormone-sensitive organs like the skeleton. Animal early-life programming models are suitable for testing the skeletal effects of pre- and neonatal exposure of soy isoflavones. This review aims to collate the impacts of early-life exposure of soy isoflavones as evidenced in animal models. The isoflavones previously studied include daidzein, genistein, or a combination of both. They were administered to rodent pups during the first few days postnatal, but prolonged exposure had also been studied. The skeletal effects were observed when the animals reached sexual maturity or after castration to induce bone loss. In general, neonatal exposure to soy isoflavones exerted beneficial effects on the skeletal system of female rodents, but the effects on male rodents seem to depend on the time of exposure and require further examinations. It might also protect the animals against bone loss due to ovariectomy at adulthood but not upon orchidectomy. The potential benefits of isoflavones on the skeletal system should be interpreted together with its non-skeletal effects in the assessment of its safety and impacts.

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The isoflavone genistein enhances osteoblastogenesis: signaling pathways involved

Cited by 24 publications

References 32 publications

Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism

Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism

Emerging Natural-Product-Based Treatments for the Management of Osteoarthritis

Skeletal Effects of Early-Life Exposure to Soy Isoflavones—A Review of Evidence From Rodent Models

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