The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene

Paul, Wyatt; Hodge, Rachel; Smartt, Sarah; Draper, John; Scott, Rod

doi:10.1007/bf00026787

Cited by 163 publications

(140 citation statements)

References 41 publications

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“…LTP45 is a homolog of Arabidopsis A9, a tapetum-specific marker for early anther developmental stages (Paul et al, 1992). LTP45 was preferentially expressed in tapetal cells at stage 7 and stage 8 in wild-type anthers, whereas in tip2 anthers its expression was not detectable ( Figures 3G to 3N), suggesting that tip2 has defects in tapetal cell identity specification.…”

Section: Identification Of the Tip2 Mutantmentioning

confidence: 99%

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

et al. 2014

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In male reproductive development in plants, meristemoid precursor cells possessing transient, stem cell-like features undergo cell divisions and differentiation to produce the anther, the male reproductive organ. The anther contains centrally positioned microsporocytes surrounded by four distinct layers of wall: the epidermis, endothecium, middle layer, and tapetum. Here, we report that the rice (Oryza sativa) basic helix-loop-helix (bHLH) protein TDR INTERACTING PROTEIN2 (TIP2) functions as a crucial switch in the meristemoid transition and differentiation during early anther development. The tip2 mutants display undifferentiated inner three anther wall layers and abort tapetal programmed cell death, causing complete male sterility. TIP2 has two paralogs in rice, TDR and EAT1, which are key regulators of tapetal programmed cell death. We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1. In addition, TIP2 can interact with TDR, indicating a role of TIP2 in later anther development. Our findings suggest that the bHLH proteins TIP2, TDR, and EAT1 play a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers, highlighting the role of paralogous bHLH proteins in regulating distinct steps of plant cell-type determination.

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Section: Identification Of the Tip2 Mutantmentioning

confidence: 99%

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

et al. 2014

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“…For time-course and expression analysis, the female Col-0 (seed stock ID CS6673) and C24 (seed stock ID CS22620) diploid ecotypes of Arabidopsis thaliana were hemizygous for a male sterility construct (Paul et al, 1992) and were gifts from Rod Scott (University of Bath, UK). Diploid Arabidopsis arenosa var Strecno was originally collected by Marcus Koch near Strecno Castle in Slovakia and was provided to us by M. Lysak.…”

Section: Plant Growthmentioning

confidence: 99%

Early Disruption of Maternal–Zygotic Interaction and Activation of Defense-Like Responses inArabidopsisInterspecific Crosses

et al. 2013

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Seed death resulting from hybridization between Arabidopsis thaliana and Arabidopsis arenosa has complex genetic determination and involves deregulation 5 to 8 d after pollination (DAP) of AGAMOUS-LIKE genes and retroelements. To identify causal mechanisms, we compared transcriptomes of compatible and incompatible hybrids and parents at 3 DAP. Hybrids misexpressed endosperm and seed coat regulators and hyperactivated genes encoding ribosomal, photosynthetic, stress-related, and immune response proteins. Regulatory disruption was more severe in Columbia-0 hybrids than in C24 hybrids, consistent with the degree of incompatibility. Maternal loss-of-function alleles for endosperm growth factor TRANSPARENT TESTA GLABRA2 and HAIKU1 and defense response regulators NON-EXPRESSOR OF PATHOGENESIS RELATED1 and SALICYLIC ACID INDUCTION-DEFICIENT2 increased hybrid seed survival. The activation of presumed POLYCOMB REPRESSIVE COMPLEX (PRC) targets, together with a 20-fold reduction in expression of FERTILIZATION INDEPENDENT SEED2, indicated a PRC role. Proximity to transposable elements affected natural variation for gene regulation, but transposon activation did not differ from controls. Collectively, this investigation provides candidates for multigenic orchestration of the incompatibility response through disruption of endosperm development, a novel role for communication between endosperm and maternal tissues and for pathways previously connected to immunity, but, surprisingly, does not identify a role for transposons.

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“…Arabidopsis thaliana plants used were C24 diploid (2x) hemizygous for an A9 barnase construct, which confers male sterility (Paul et al, 1992), and C24 tetraploid (4x) A9 barnase. Male-sterile segregants were used as seed parents in crosses, and their male-fertile siblings were used as pollen parents.…”

Section: Plant Materialsmentioning

confidence: 99%

The Basis of Natural and Artificial Postzygotic Hybridization Barriers in Arabidopsis Species

2003

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The success or failure of interspecific crosses is vital to evolution and to agriculture, but much remains to be learned about the nature of hybridization barriers. Several mechanisms have been proposed to explain postzygotic barriers, including negative interactions between diverged sequences, global genome rearrangements, and widespread epigenetic reprogramming. Another explanation is imbalance of paternally and maternally imprinted genes in the endosperm. Interspecific crosses between diploid Arabidopsis thaliana as the seed parent and tetraploid Arabidopsis arenosa as the pollen parent produced seeds that aborted with the same paternal excess endosperm phenotype seen in crosses between diploid and hexaploid A. thaliana . Doubling maternal ploidy restored seed viability and normal endosperm morphology. However, substituting a hypomethylated tetraploid A. thaliana seed parent reestablished the hybridization barrier by causing seed abortion and a lethal paternal excess phenotype. We conclude from these findings that the dominant cause of seed abortion in the diploid A. thaliana ϫ tetraploid A. arenosa cross is parental genomic imbalance. Our results also demonstrate that manipulation of DNA methylation can be sufficient to erect hybridization barriers, offering a potential mechanism for speciation and a means of controlling gene flow between species.

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The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene

Cited by 163 publications

References 41 publications

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

Early Disruption of Maternal–Zygotic Interaction and Activation of Defense-Like Responses inArabidopsisInterspecific Crosses

The Basis of Natural and Artificial Postzygotic Hybridization Barriers in Arabidopsis Species

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