The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family

Maddon, Paul J.; Littman, Dan R.; Godfrey, Maurice; Maddon, Douglas E.; Chess, Leonard

doi:10.1016/s0092-8674(85)80105-7

Cited by 647 publications

(347 citation statements)

References 64 publications

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“…For confirmation of the purity of enriched samples, the cDNA from sorted DN ~x/[3 T cells was amplified by P C R with CD4 and CD8tx primers (15), and hybridized with the 32P-labeled EcoKI fragment of the human CD4 gene (16) or the PstI/HincII fragment of the human CD8Ot gene (17). The CD4 or CD8 cDNA (1 ng) was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

Selective reduction of T cells bearing invariant V alpha 24J alpha Q antigen receptor in patients with systemic sclerosis.

Sumida

Sakamoto

Murata

et al. 1995

The Journal of Experimental Medicine

289

178

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S Ul-lfuTlary A novel subset ofT ceils characterized by the expression of an invariant T cell antigen receptor (TC1L) encoded by V0t24J~xQ gene segments was investigated in patients with systemic sclerosis (SSc). Polymerase chain reaction analysis demonstrated that the Vot24 TClL repertoire was selectively used in CD4-CD8-double-negative T cells both in patients and in healthy individuals, while almost all families of TCR.Vot were expressed in single-positive T cell fractions. The Vor + double-negative T cells were increased by approximately fivefold in patients. However, sequence analysis clearly showed significant differences in the Vo~24 TC1L repertoire dominating in patients and healthy donors. In healthy individuals, the invariant Vot24JoLQ was expanded and comprised 20-50% of the total TCR-ot, while their selective reduction was observed in SSc patients who also showed expansion of invariant Vex24 TCR other than Vot24JcxQ. Analogous to murine invariant V~14JoL281 TC1L, these results suggest that T cells with invariant V&24JotQ TCR would function as regulatory T cells, whereas T cells bearing other invariant Vex24 TCR in SSc patients could be autoaggressive T cells in nature. Recent studies by Porcelli et al. (6) and Dellabona et al. (7,8) indicate that the invariant Vcx24J~xQ sequence is preferentially expressed on DN ot/]3 T cells from healthy individuals. The homology of the nucleotide sequences is found to be 75% in the V0t24 and 90% in the CDR3 regions compared with murine Vo~24 TCR (9). Therefore, the human V&24 sequence is a homologue of murine V&14 TCP,. Another striking similarity to murine invariant V~14 T cells is that human peripheral DN T cells also express a limited TCR.-[3 repertoire including V [32, V[38, VI311,10). Interestingly, the decrease in invariant Vod4Jo~281 TCR expression in autoimmune prone mice correlates with disease development. It is thus likely that Vot14 § NK T cells play a role in the regulation ofautoimmune disease development.

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Section: Methodsmentioning

confidence: 99%

Selective reduction of T cells bearing invariant V alpha 24J alpha Q antigen receptor in patients with systemic sclerosis.

Sumida

Sakamoto

Murata

et al. 1995

The Journal of Experimental Medicine

289

178

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“…This molecule plays an important role in the development and the activation of T cells by increasing the affinity/ avidity of TCR for the peptide/class II molecule of MHC II and/or by transducing signals through the associated tyrosine kinase p56 lck (1)(2)(3)(4). The extracellular part of CD4 consists of four domains (D1-D4) with Ig-like structures and is involved in the interaction with class II molecules of the MHC and other ligands (1,2,5).…”

mentioning

confidence: 99%

CD4 Dimers Constitute the Functional Component Required for T Cell Activation

Moldovan

Yachou

Lévesque³

et al. 2002

The Journal of Immunology

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The CD4 molecule plays a key role in the development and activation of helper T cells. Dimerization and oligomerization is often a necessary step in the function of several cell surface receptors. Herein, we provide direct biochemical evidence confirming the presence of CD4 as dimers in transfected cells from hemopoetic and fibroblastic origin as well as in primary T cells. Such dimers are also observed with murine CD4 confirming selective pressure during evolution to maintain such a structure. Using a series of point mutations, we have precisely mapped the dimerization site at residues K318 and Q344 within the fourth extracellular domain of CD4. These residues are highly conserved and their mutation results in interference with dimer formation. More importantly, we demonstrate that dimer formation is essential for the coligand and coreceptor functions of CD4 in T cell activation. These data strongly suggest that CD4 dimerization is necessary for helper T cell function.

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“…These PCR products, including CD4 and CD8a genes, yielded a band of 200 basepairs and 134 bp, respectively. Amplified DNA were separated by agarose gel and were hybridized with the '*P-labeled Eco RI fragment of the human CD4 gene (9) or the Pst IIHinc I1 fragment of the human CD8a gene (10). The cDNA (1 ng) encoding CD4 or CD8 genes were used for PCR as a positive control.…”

Section: Methodsmentioning

confidence: 99%

T cell receptor vβ repertoire of double‐negative α/β t cells in patients with systemic sclerosis

Sakamoto

Sumida²,

Maeda³

et al. 1992

Arthritis & Rheumatism

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The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family

Cited by 647 publications

References 64 publications

Selective reduction of T cells bearing invariant V alpha 24J alpha Q antigen receptor in patients with systemic sclerosis.

Selective reduction of T cells bearing invariant V alpha 24J alpha Q antigen receptor in patients with systemic sclerosis.

CD4 Dimers Constitute the Functional Component Required for T Cell Activation

T cell receptor vβ repertoire of double‐negative α/β t cells in patients with systemic sclerosis

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