The isolation and prevalence of campylobacters from dairy cattle using a variety of methods

Atabay, H.I.; Corry, Janet E L

doi:10.1046/j.1365-2672.1998.00402.x

Cited by 85 publications

(61 citation statements)

References 31 publications

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“…Since lpxA encodes UDP-GlcNAc acyltransferase, an enzyme required for lipid A biosynthesis (56), partial mutation of the lpxA gene in the primer annealing region is considered more likely than deletion of the gene. Given the composition of isolation media and the isolation conditions used, isolation of C. hyointestinalis is plausible and has been observed by other investigators (2,8,21,31). Although the isolation of thermotolerant C. fetus was fortuitous, other investigators have reported the isolation of similar atypical C. fetus strains from human and raw milk (13,32,62).…”

Section: Discussionmentioning

confidence: 96%

“…The media and isolation conditions used in this study were primarily developed for isolating C. jejuni and C. coli, and this may have biased detection in favor of these two species (2,5,11,15). Nevertheless, the prevalence of thermophilic campylobacters (C. jejuni, 34%; C. coli, 8%; other, 2%) in this study is generally concordant with the previous studies in which the prevalence of campylobacters in cattle ranged from 0.8 to 46.7%, depending on the isolation methods, herd size and type, geography, season, animal age, and number of animals investigated (9,21,28,41,47,61).…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter spp. from Cattle Farms in Washington State

Bae

Kaya

Hancock

et al. 2005

Appl Environ Microbiol

105

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The prevalence of thermophilic Campylobacter spp. was investigated in cattle on Washington State farms. A total of 350 thermophilic Campylobacter isolates were isolated from 686 cattle sampled on 15 farms (eight dairies, two calf rearer farms, two feedlots, and three beef cow-calf ranches). Isolate species were identified with a combination of phenotypic tests, hipO colony blot hybridization, and multiplex lpxA PCR. Breakpoint resistance to four antimicrobials (ciprofloxacin, nalidixic acid, erythromycin, and doxycycline) was determined by agar dilution. Campylobacter jejuni was the most frequent species isolated (34.1%), followed by Campylobacter coli (7.7%) and other thermophilic campylobacters (1.5%). The most frequently detected resistance was to doxycycline (42.3% of 350 isolates). Isolates from calf rearer facilities were more frequently doxycycline resistant than isolates from other farm types. C. jejuni was most frequently susceptible to all four of the antimicrobial drugs studied (58.8% of 272 isolates). C. coli isolates were more frequently resistant than C. jejuni, including resistance to quinolone antimicrobials (89.3% of isolates obtained from calves on calf rearer farms) and to erythromycin (72.2% of isolates obtained from feedlot cattle). Multiple drug resistance was more frequent in C. coli (51.5%) than in C. jejuni (5.1%). The results of this study demonstrate that C. jejuni is widely distributed among Washington cattle farms, while C. coli is more narrowly distributed but significantly more resistant.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter spp. from Cattle Farms in Washington State

Bae

Kaya

Hancock

et al. 2005

Appl Environ Microbiol

105

View full text Add to dashboard Cite

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“…Others have previously isolated these two campylobacters from cattle at various frequencies (e.g., see references 3, 6, 16, 19, and 55). All media used to isolate Campylobacter species select for specific taxa and even genotypes (3,5,13,28,47). As a result, microbiological methods that employ selective media do not necessarily provide a true measure of the frequency and diversity of Campylobacter species associated with livestock and their feces.…”

Section: Discussionmentioning

confidence: 99%

Effects of Subtherapeutic Administration of Antimicrobial Agents to Beef Cattle on the Prevalence of Antimicrobial Resistance in Campylobacter jejuni and Campylobacter hyointestinalis

Inglis

McAllister

Busz

et al. 2005

Appl Environ Microbiol

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The influence of antimicrobial agents on the development of antimicrobial resistance (AMR) in Campylobacter isolates recovered from 300 beef cattle maintained in an experimental feedlot was monitored over a 315-day period (11 sample times). Groups of calves were assigned to one of the following antimicrobial treatments: chlortetracycline and sulfamethazine (CS), chlortetracycline alone (Ct), virginiamycin, monensin, tylosin phosphate, and no antimicrobial agent (i.e., control treatment). In total, 3,283 fecal samples were processed for campylobacters over the course of the experiment. Of the 2,052 bacterial isolates recovered, 92% were Campylobacter (1,518 were Campylobacter hyointestinalis and 380 were C. jejuni). None of the antimicrobial treatments decreased the isolation frequency of C. jejuni relative to the control treatment. In contrast, C. hyointestinalis was isolated less frequently from animals treated with CS and to a lesser extent from animals treated with Ct. The majority (>94%) of C. jejuni isolates were sensitive to ampicillin, erythromycin, and ciprofloxacin, but more isolates with resistance to tetracycline were recovered from animals fed Ct. All of the 1,500 isolates of C. hyointestinalis examined were sensitive to ciprofloxacin. In contrast, 11%, 10%, and 1% of these isolates were resistant to tetracycline, erythromycin, and ampicillin, respectively. The number of animals from which C. hyointestinalis isolates with resistance to erythromycin and tetracycline were recovered differed among the antimicrobial treatments. Only Ct administration increased the carriage rates of erythromycinresistant isolates of C. hyointestinalis, and the inclusion of CS in the diet increased the number of animals from which tetracycline-resistant isolates were recovered. The majority of C. hyointestinalis isolates with resistance to tetracycline were obtained from cohorts within a single pen, and most of these isolates were recovered from cattle during feeding of a forage-based diet as opposed to a grain-based diet. The findings of this study show that the subtherapeutic administration of tetracycline, alone and in combination with sulfamethazine, to feedlot cattle can select for the carriage of resistant strains of Campylobacter species. Considering the widespread use of in-feed antimicrobial agents and the high frequency of beef cattle that shed campylobacters, the development of AMR should be monitored as part of an on-going surveillance program.

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“…The digestive tract of clinically normal cattle has been demonstrated to be a significant reservoir for a number of Campylobacter spp. [12], with prevalences of the enteropathogen in cattle ranging from 0-80%. Prevalences of Campylobacter in sheep have been shown to be generally lower with approximately 20% of animals intestinal carriers [197].…”

Section: Campylobacters and Other Food Animalsmentioning

confidence: 99%

Campylobacter

et al. 2005

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-Species within the genus, Campylobacter, have emerged over the last three decades as significant clinical pathogens, particularly of human public health concern, where the majority of acute bacterial enteritis in the Western world is due to these organisms. Of particular concern are the species, C. jejuni and C. coli, which are responsible for most of these gastrointestinal-related infections. Although these organisms have already emerged as causative agents of zoonoses, several aspects of their epidemiology and pathophysiology are only beginning to emerge. Trends in increasing antibiotic resistance are beginning to emerge with oral antibiotics, which may be the drug of choice for when it is necessary to intervene chemotherapeutically. This review wishes to examine (i) emerging clinical aspects of the disease, such as Guillain Barré syndrome (GBS), (ii) the association between these organisms and poultry as a natural host, (iii) environmental aspects of Campylobacter epidemiology, (iv) the emergence of atypical campylobacters (v) emerging trends in antibiotic resistance, (vi) adoption of modern methods for the detection of campylobacters.

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The isolation and prevalence of campylobacters from dairy cattle using a variety of methods

Cited by 85 publications

References 31 publications

Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter spp. from Cattle Farms in Washington State

Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter spp. from Cattle Farms in Washington State

Effects of Subtherapeutic Administration of Antimicrobial Agents to Beef Cattle on the Prevalence of Antimicrobial Resistance in Campylobacter jejuni and Campylobacter hyointestinalis

Campylobacter

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