The Isolation of a New Virus Associated With Respiratory Clinical Disease in Humans

Price, Winston H.

doi:10.1073/pnas.42.12.892

Cited by 148 publications

(74 citation statements)

References 4 publications

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“…The first HRV was discovered in 1956 1 . At the end of the 80s, 100 serotypes were identified using susceptible cell culture and specific antisera 2 .…”

mentioning

confidence: 99%

Spread of different rhinovirus B genotypes in hospitalized children in Spain

Cuevas

Molinero

Pozo

et al. 2012

Influenza Resp Viruses

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Please cite this paper as: Cuevas et al. (2013) Spread of different rhinovirus B genotypes in hospitalized children in Spain. Influenza and Other Respiratory Viruses 7(5), 623–628.Human Rhinovirus (HRV) classification is an evolving process. New genotypes have been described within HRV‐A and HRV‐C species, but only one has been accepted related to HRV‐B. From 2003 to 2010, a total of 3987 nasopharyngeal aspirate samples were taken from pediatric patients admitted to the Severo Ochoa Hospital in Madrid (Spain). After viral analysis, 949 (23·8%) tested positive to HRV. A random selection of 397 (42%) positive samples showed that 39 (9·8%) were HRV‐B. The sequencing of partial VP4/VP2 coding region revealed the spread of 13 of 25 defined HRV‐B serotypes and three putative new genotypes. Such results remark the high diversity of HRV‐B.

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“…The first HRV was discovered in 1956 1 . At the end of the 80s, 100 serotypes were identified using susceptible cell culture and specific antisera 2 .…”

mentioning

confidence: 99%

Spread of different rhinovirus B genotypes in hospitalized children in Spain

Cuevas

Molinero

Pozo

et al. 2012

Influenza Resp Viruses

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“…For example, influenza viruses are cultivated in MDCK cells (12,18), parainfluenzaviruses in primary simian and human kidney cell cultures (1, 2, 4), Vero cells (14) or LLC-MK cells (5), respiratory syncytial (RS) virus in HEp-2 cells (9) or Bristol Hela cells (11), adenoviruses in a wide range of human established cell lines (6) and rhinoviruses in human diploid cells (15,16). For this reason, virus isolation for ARI is cumbersome and expensive, and simplification of the tissue culture method for virus isolation is desirable.…”

mentioning

confidence: 99%

A Microplate Method for Isolation of Viruses from Infants and Children with Acute Respiratory Infections

Numazaki

Oshima

Ohmi

et al. 1987

Microbiology and Immunology

105

103

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Between December 1984 and December 1986, a microplate technique was adopted for isolation of viruses from infants and children with acute respiratory infections. By using two kinds of tissue culture microplates, i.e., the HHVM plate, containing human embryonic fibroblast (HEF), HEp-2, Vero and MDCK cells, and the MK plate which contains secondary monkey kidney cells, 1,080 field viruses were isolated from 1,061 (24.9%) out of 4,254 throat swabs. Of these 1,080 isolates, 1,003 (92.9%) were recovered in the HHVM plates and the remaining 77 (7.1%) in the MK plates. With the HHVM plate, influenza A and B viruses were cultivated in MDCK, RS virus in HEp-2, parainfluenza and mumps viruses in Vero, adenoviruses in both HEF and HEp-2, polioviruses in HEF, HEp-2 and Vero, coxsackie B viruses in both HEp-2 and Vero, rhino and echo viruses in HEF, herpes simplex virus in both HEF and HEp-2, and cytomegalovirus in HEF, although MK were more sensitive than Vero to parainfluenza and coxsackie B viruses. There was no difference in the rate of isolation of viruses between the microplate and ordinary tube methods. Cross contamination in the microplates was negligible for routine work.

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“…agents have already been reported to be isolated from upper respiratory illnesses (Price, 1956;Chanock, 1956;Pelon, Mogabgab, Phillips and Pierce, 1957) besides adenovirus group. During the studies of adenovirus infections and other respiratory illnesses, a cytopathogenic agent has been isolated by the present authors from an infant suffering from a febrile illness accompanied with pharyngitis.…”

Section: Several Cytopathogenicmentioning

confidence: 99%