The Isolation of Antennal Mutants and their use in Drosophila Olfactory Genetics

Ayer, Richard K.; Monte, Paula; Carlson, John R.

doi:10.1007/978-1-4899-2519-0_27

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…1984;Crnjar et al, 1989;Alcorta, 1991 ). Flies were held during recording in a 100-p1 pipet tip, as described in Ayer et al (1989). The fly was inserted into the pipet tip using a mouth aspirator; the end of the tip was trimmed such that the anterior portion of the fly's head, bearing the antennae, just protruded.…”

Section: Electricalmentioning

confidence: 99%

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

1992

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This article provides characterization of the electrical response to odorants in the Drosophila antenna and provides physiological evidence that a second organ, the maxillary palp, also has olfactory function in Drosophila. The acj6 mutation, previously isolated by virtue of defective olfactory behavior, affects olfactory physiology in the maxillary palp as well as in the antenna. Interestingly, abnormal chemosensory jump 6 (acj6) reduces response in the maxillary palp to all odorants tested except benzaldehyde (odor of almond), as if response to benzaldehyde is mediated through a different type of odorant pathway from the other odorants. In other experiments, different parts of the antenna are shown to differ with respect to odorant sensitivity. Evidence is also provided that antennal response to odorants varies with age, and that odorants differ in their age dependence.

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Section: Electricalmentioning

confidence: 99%

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

1992

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“…Thus, the reduced chemosensory jump response in acj6 adults may be a function of a reduced antennal odorant response. Light microscopy of the antennal surface (23,35) reveals no obvious defects in gross morphology or in the pattern of olfactory sensilla to account for reduced EAG responses or abnormal olfactory behavior.…”

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confidence: 98%

acj6: a gene affecting olfactory physiology and behavior in Drosophila.

Ayer

Carlson

1991

Proc. Natl. Acad. Sci. U.S.A.

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Mutations affecting olfactory behavior provide material for use in molecular studies of olfaction in Drosophila melanogaster. Using the electroantennogram (EAG), a measure of antennal physiology, we have found an adult antennal defect in the olfactory behavioral mutant abnormal chemosensory jump 6 (acj6). The acj6 EAG defect was mapped to a single locus and the same mutation was found to be responsible for both reduction in EAG amplitude and diminished behavioral response, as if reduced antennal responsiveness to odorant is responsible for abnormal chemosensory behavior in the mutant. acj6 larval olfactory behavior is also abnormal; the mutation seems to alter cellular processes necessary for olfaction at both developmental stages. The acj6 mutation exhibits specificity in that visual system function appears normal in larvae and adults. These experiments provide evidence that the acj6 gene encodes a product required for olfactory signal transduction.

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“…Forward genetic approaches and quantitative genetic and genomic analyses have yielded insights about the genetic architecture underlying olfactory behavior (Anholt et al, 2003; Anholt et al, 2001; Ayer and Carlson, 1991; Ayer et al, 1989; Fedorowicz et al, 1998; Rollmann et al, 2005). One of the first transcription factors that was shown to play a role in Or gene choice, Acj6, was identified using a forward genetic screen in Drosophila melanogaster for mutants that affected responses to a specific subset of odors tested (Ayer and Carlson, 1991).…”

Section: Introductionmentioning

confidence: 99%

Disruption of olfactory receptor neuron patterning in Scutoid mutant Drosophila

Tom

Bruyne

Haehnel

et al. 2011

Molecular and Cellular Neuroscience

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Olfactory neurons show an extreme diversity of cell types with each cell usually expressing one member from a large family of 60 Odorant receptor (Or)genes in Drosophila. Little is known about the developmental processes and transcription factors that generate this stereotyped pattern of cellular diversity. Here we investigate the molecular and cellular basis of defects in olfactory system function in an unusual dominant mutant, Scutoid. We show that the defects map to olfactory neurons innervating a specific morphological class of sensilla on the antenna, large basiconics. Molecular analysis indicates defects in neurons expressing specific classes of receptor genes that map to large basiconic sensilla. Previous studies have shown that in Scutoid mutants the coding region of the transcriptional repressor snail is translocated near the no-ocelli promoter, leading to misexpression of snail in the developing eye-antenna disc. We show that ectopic expression of snail in developing olfactory neurons leads to severe defects in neurons of the antennal large basiconics supporting the model that the dominant olfactory phenotype in Scutoid is caused by misexpression of snail.

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The Isolation of Antennal Mutants and their use in Drosophila Olfactory Genetics

Cited by 4 publications

References 14 publications

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

acj6: a gene affecting olfactory physiology and behavior in Drosophila.

Disruption of olfactory receptor neuron patterning in Scutoid mutant Drosophila

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