The Isolation of the Mouse Nerve Growth Factor Protein in a High Molecular Weight Form<sup>*</sup>

Varon, Silvio; Nomura, Junichi; Shooter, Eric M.

doi:10.1021/bi00859a043

Cited by 364 publications

(140 citation statements)

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“…This tissue contains exceptionally large amounts of NGF [8]. The neurotrophic molecule is a dimer, called ,&NGF, whieh is associated with two other subunits, LY and y [9]. The biosynthesis of the subm~ll~y gland NGF is primarily controlled by testosterone, but is also stimulated by the thyroid hormone T4 [lo-121. …”

Section: Introductionmentioning

confidence: 99%

Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

et al. 1985

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Mouse L cells synthesize and secrete a neurotrophic fztor related to the /J subunit of the submaxillary gland nerve growth factor (NGF) of male mice. Use of a cDNA probe which encodes the p-NGF mRNA demonstrated that L cells produce a transcript identical in size to that of the submaxillary gland. Moreover, target sites of restriction enzymes EcoRI, PsrI and BarnHI were not significantly rearranged in the &NGF gene locus of these cells. The abundant of the &NGF transcript was found to depend on culture conditions. Removal of serum depressed the cellular content of polyadenylated RNA by a factor of 1.7, and deereased specifically the pool of fl-NGF transcript by an additional factor of 4, The presence of IO-' M testosterone in the serum-free medium did not modify the level of /?-NGF mRNA, while addition of lo-' M T3 (or T4) increased this Ievel by a factor of 1.5. These data provide the first evidence that the @-NGF mRNA of L c&s is subjected to regulation, but in a way apparently different from that described for the submaxillary gland.

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Section: Introductionmentioning

confidence: 99%

Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

et al. 1985

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“…These two forms arise because two different schemes are used in their purification from gland extracts. One of these, called 2.5S NGF (3), has a molecular weight of 26,000, and the other, termed 7S NGF (4), has a molecular weight of approximately 140,000. These two proteins are related in that 7S NGF contains as part of its subunit structure a component that is closely similar to 2.5S NGF (4).…”

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confidence: 99%

“…One of these, called 2.5S NGF (3), has a molecular weight of 26,000, and the other, termed 7S NGF (4), has a molecular weight of approximately 140,000. These two proteins are related in that 7S NGF contains as part of its subunit structure a component that is closely similar to 2.5S NGF (4). In solution at relatively high protein concentrations, 7S NGF is unstable, and it dissociates to yield a mixture of its component polypeptide chains (5,6).…”

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confidence: 99%

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Nerve growth factor: a protease that can activate plasminogen.

Orenstein¹,

Dvorak²,

Blanchard³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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The single, highly stable form of mouse submandibular gland nerve growth factor (NGF), prepared as described by Young eta. [(1978) Biochemistry 17, 1490-1498] is a protease of restricted specificity that can convert plasminogen to plasmin. In the absence of plasminogen, NGF is not fibrinolytic, nor does it hydrolyze casein at a measurable rate. Treatment of NGF with diisopropyl fluorophosphate inhibits its ability to activate plasminogen as well as its capacity to hydrolyze certain synthetic argnine esters. These results indicate that NGF is a member of the class of serine proteases. Since

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“…The first amino-acid analyses reported indicated that the protein contained 265 amino acids and had an apparent isoelectric point of 9.3 (8). An additional degree of complexity concerning the relation of structural and functional properties of NGF was introduced with the report that the factor is associated with at least two other proteins (denoted a and y) when it is first released from the tissue (9).…”

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confidence: 99%

Nerve Growth Factor from Mouse Submaxillary Gland: Amino Acid Sequence

Angeletti

Bradshaw

1971

Proc. Natl. Acad. Sci. U.S.A.

346

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The complete amino-acid sequence of the 2.5S nerve growth factor from male-mouse submaxillary glands has been determined. The unambiguous alignment of peptides derived from tryptic, chyrmotryptic, thermolytic, and peptic digestion of S-carboxymethyl-, S-aminoetlhyl-, and native growth factor indicates that the prinary subunit is composed of 118 amino acids, with aminoterminal serine and carboxyl-terminal arginine. The molecular weight of this subunit, calculated from the primary sequence, is 13,259. Thus, the native protein, which is composed of two of the subunits, has a molecular weight of 26,518. These values, as well as the final aminoacid composition, are in excellent agreement with those determined by direct measurement with undigested growth factor. The alignment of the three disulfide bonds, determined from a combination of peptic and thermolytic digestions, is I-IV, II-V, and III-VI. The latter two pairs are located in a closed loop of 14 amino acids, by virtue of the fact that half-cystinyl residues V and VI are separated by only a single residue in the linear sequence. Assignment of the side-chain amides showed that 7 of 11 aspartic acid residues and 2 of 8 glutamic acid residues are present as amides. This distribution of charged residues is entirely consistent with the observed isoelectric point of 9.3.

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The Isolation of the Mouse Nerve Growth Factor Protein in a High Molecular Weight Form^*

Cited by 364 publications

References 13 publications

Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

Nerve growth factor: a protease that can activate plasminogen.

Nerve Growth Factor from Mouse Submaxillary Gland: Amino Acid Sequence

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The Isolation of the Mouse Nerve Growth Factor Protein in a High Molecular Weight Form*

Cited by 364 publications

References 13 publications

Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

Nerve growth factor: a protease that can activate plasminogen.

Nerve Growth Factor from Mouse Submaxillary Gland: Amino Acid Sequence

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The Isolation of the Mouse Nerve Growth Factor Protein in a High Molecular Weight Form^*