The isomerization ofD-glucose intoD-fructose catalyzed by whole-cell immobilized glucose isomerase. The dependence of the intrinsic rate of reaction on substrate concentration, pH, and temperature

Kikkert, A.; Vellenga, K.; Wilt, H.G.J. de; Joosten, G.E.H.

doi:10.1002/bit.260230516

Cited by 17 publications

(12 citation statements)

References 16 publications

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“…Van't Hoff plot of equilibrium constants. + , Takasaki et al (1%9); A,Sproull et al (1976); ,Lee et al (1976); v ,Lloyd and Khaleeluddin (1976); X,Kikkert et al (1981); 0 , Park et al (1981); v ,Chen et al (1983); 0 ,Benaiges et al (1986); 0 ,Chen and Wu (1987); rn ,Visuri and Klivanov (1987); A , Gram et al (1990); 0 , Present Study; ---Roels and Van Tilberg (1979) equation; -Equation (17). comparison of this equation with the experimental results indicates that the value of K is practically zero, and thus Kmf = Kmg.…”

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confidence: 99%

Kinetic study of fructose‐glucose isomerization in a recirculation reactor

et al. 1995

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We have studied the equilibrium and kinetics of fructose-to-glucose isomerhtion using a commercial immobilized glucose isomerase, Sweetzyme P (from NOVO), in a packed-bed recirculation reactor which enabled US to eliminate the influence of external-mass transfer and which could function as a differential reactor when a sufficient flow rate was used. 'Ihe results obtained have alsobeen comcted with regad tothe Muence of iaernal transport in the immobilized enzyme particles.Isomerization kinetics is a pseudo iirst-order reversible reaction in the ranges studied: 303-333 K, pH 7.5 and 0.5 to 2.0 moYL hexose concentrations. We compare our results with those found in the literature, and propose equations for the influence of temperature on the equilibrium and on the kinetic constants.Nous avons kNdik I'kquilibre et la cinktique de l'isomkrisation du fructose en glucose h l'aide d'une isodrase de glucose immobiliske commerciale, Sweetzyme P (de NOVO), dans un rcacteUr h recirculation h lit g a d ; ce rhcteur nous a permis d'kliminer l'influence du transfert de matibre externe et pouvait fonctionner comme t.eacteur diffkrential si on appliquait un dkbit suffisant. Les r&ultats obtenus ont kgalement ktt? ConigeS en ce qui concern I'influence du transport interne dans les particules d'enzyme immobilish.La cinktique d'isomkrisation est une rhction dversible du pseudo-premier order dans les gammes 6tudiQs, h savoir: 303-333 K, pH 7 3 et concentration d'hexose de 0.5 h 2.0 mom. Nous avons cornpark nos dsultats avec ceux de la litt6rature scientifique et nous pmposons des &pations pour I'innuenCe de la tempbme sur I'6qdibn et les constantes cinktiques.

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confidence: 99%

Kinetic study of fructose‐glucose isomerization in a recirculation reactor

et al. 1995

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“…By estimating the initial rates of the glucose to fructose and vice versa, these parameters can be determined using the Lineweaver-Menten equation. 20 However, one can evaluate these four key parameters (i.e., v mg , v mf , k mg , and k mf ) by carrying out just experiments for glucose to fructose reaction. Rubio et al have rewritten the rate of reaction as follows 21 :…”

Section: Reaction Kineticsmentioning

confidence: 99%

Experimental and modeling study of catalytic reaction of glucose isomerization: Kinetics and packed‐bed dynamic modeling

2008

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in Wiley InterScience (www.interscience.wiley.com).The kinetics and equilibrium of isomerization reaction of D-glucose to D-fructose have been investigated using a commercial immobilized glucose isomerase (IGI), Sweetzyme type IT 1 , in a batch stirred-tank reactor. The batch experimental data were used to model the reaction kinetics using the well-known Michaelis-Menten rate expression. The kinetic model was utilized in a dynamic-mathematical model for a packed-bed reactor to predict the concentration profiles of D-glucose and D-fructose within the reactor. The experimental results for the fractional conversion of D-glucose in the packed-bed reactor of IGI catalyst indicated that the model prediction of the transient and steady-state performance of the packed-bed reactor was satisfactory and as such could be used in the design of a fixed-bed IGI catalytic reactor. Moreover, the influences of axial mixing term, particle Re number, and axial peclet number (Pe a ) on the performance capability of the packed-bed reactor of IGI catalyst were investigated.

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“…From the kinetic point of view, glucose to fructose isomerization is a reversible reaction that is characterized by formation of an intermediate complex [16], [17].…”

Section: Introductionmentioning

confidence: 99%

Kinetics of a Three-Step Isomerization of Glucose to Fructose Using Immobilized Enzyme

Gaily¹,

Sulieman²,

Abasaeed³

2013

IJCEA

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Abstract-A three-step complex formation of fructose by glucose isomerization was suggested to describe the isomerization kinetics. The model was characterized by the formation of a fructose complex. Experimental data obtained from the isomerization process using immobilized Sweetzyme enzyme, IT® were used in this study. Experiments were conducted at different reaction temperatures in the range of 50-70°C and glucose initial concentrations of 10, 15 and 20% and enzyme loading of 1g. Glucose concentrations dropped with time until equilibrium was reached. A first order kinetics for the steps was employed and Runge Kutta 4th order algorithm combined with a least square method were used to estimate the pre-experimental factor and activation energy for determination of the corresponding rate constants by solving the initial value problem of the suggested model using EZ-Solve software. Very good fits between experimental data and model prediction was obtained.

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The isomerization ofD-glucose intoD-fructose catalyzed by whole-cell immobilized glucose isomerase. The dependence of the intrinsic rate of reaction on substrate concentration, pH, and temperature

Cited by 17 publications

References 16 publications

Kinetic study of fructose‐glucose isomerization in a recirculation reactor

Kinetic study of fructose‐glucose isomerization in a recirculation reactor

Experimental and modeling study of catalytic reaction of glucose isomerization: Kinetics and packed‐bed dynamic modeling

Kinetics of a Three-Step Isomerization of Glucose to Fructose Using Immobilized Enzyme

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