We present the kinetic parameters and equilibrium constant of the enzymatic glucose−fructose isomerization reaction with an immobilized glucose isomerase (IGI), Sweetzyme IT, using a batch stirred-tank reactor following the procedure developed by Dehkordi et al. (AIChE J.2008541333). The model predictions were compared with the experimental data and fair agreements were found. The influence of temperature on the equilibrium constant and kinetic parameters of glucose to fructose isomerization reaction was investigated. In addition, the frequency factors and the activation energies were determined by using Arrhenius-like expressions. Furthermore, correlations were found for the maximum reaction rate (i.e., V m) and apparent Michaelis−Menten constant (i.e., K m).

Novel type of two-impinging-jets loop reactor (TIJLR) has been proposed and tested successfully for the solid-liquid catalytic reactions. The TIJLR was tested using the catalytic reaction of isomerization of D-glucose to D-fructose by immobilized glucose isomerase catalyst as a typical model system of solid-liquid catalytic reactions. The TIJLR is characterized by a high intensity reaction chamber, which is separated by a perforated plate from other parts of the reactor. The perforated plate was used as a filter to keep the catalyst particles within the reaction chamber. A compartment model with two adjustable parameters was considered to describe the pattern of flow within the reaction chamber. The compartment model proposed was based on the observation made during the experimental work. The adjustable parameters of the proposed model were determined through the experimental data by minimizing the discrepancies between the model predictions and corresponding experimental data. The influences of various operating and design parameters such as jet Reynolds number, feed flow rate, initial feed concentration, catalyst loading, and the volume of the reaction chamber on the performance capability of the TIJLR were investigated. It was found that the jet Reynolds number plays an important role in the performance capability of the TIJLR.

in Wiley InterScience (www.interscience.wiley.com).The kinetics and equilibrium of isomerization reaction of D-glucose to D-fructose have been investigated using a commercial immobilized glucose isomerase (IGI), Sweetzyme type IT 1 , in a batch stirred-tank reactor. The batch experimental data were used to model the reaction kinetics using the well-known Michaelis-Menten rate expression. The kinetic model was utilized in a dynamic-mathematical model for a packed-bed reactor to predict the concentration profiles of D-glucose and D-fructose within the reactor. The experimental results for the fractional conversion of D-glucose in the packed-bed reactor of IGI catalyst indicated that the model prediction of the transient and steady-state performance of the packed-bed reactor was satisfactory and as such could be used in the design of a fixed-bed IGI catalytic reactor. Moreover, the influences of axial mixing term, particle Re number, and axial peclet number (Pe a ) on the performance capability of the packed-bed reactor of IGI catalyst were investigated.