Kinetics of Glucose Isomerization to Fructose by Immobilized Glucose Isomerase (Sweetzyme IT)

Abstract

We present the kinetic parameters and equilibrium constant of the enzymatic glucose−fructose isomerization reaction with an immobilized glucose isomerase (IGI), Sweetzyme IT, using a batch stirred-tank reactor following the procedure developed by Dehkordi et al. (AIChE J.2008541333). The model predictions were compared with the experimental data and fair agreements were found. The influence of temperature on the equilibrium constant and kinetic parameters of glucose to fructose isomerization reaction was investigated. In addition, the frequency factors and the activation energies were determined by using Arrhenius-like expressions. Furthermore, correlations were found for the maximum reaction rate (i.e., V m) and apparent Michaelis−Menten constant (i.e., K m).

A new approach for describing and solving the reversible Briggs‐Haldane mechanism using immobilized enzyme

“…In addition, Table shows the reversible kinetic parameters of the Briggs‐Haldane mechanism given by Equation as well as the experimentally obtained equilibrium constants (55–70 °C, 0.995–1.062) using free enzyme. These constants are in the range (55–65 °C, 0.850–1.170) reported by Dehkordi et al using immobilized enzyme (Sweetzyme). It can be seen that as temperature increases, the ratio of fructose/glucose concentration also increases.…”

Ketoisomeric conversion of glucose derived from microalgal biomasses

“…In order to obtain the required fructose concentration above 50%, it has been theorized that GI which can operate at high temperature and lower pH about pH 5.0 due to the isomerization equilibrium [19][20][21]. Thus, researchers have sought to identify novel microorganism with high level of enzyme activity and thermal stability at the required conditions.…”

“…Moreover, GI is known as Xylose isomerase, which converts d-xylose to d-xylulose in vivo [3,12,20]. This isomerization is a reversible reaction [21]. GI is usually used in an immobilized form in the food industry, creating a biocatalyst that can be reused repeatedly several times at minimum costs for the operation.…”

Potenzial von Prozessintegration mit Adsorbern zur Herstellung seltener Monosaccharide – eine modellbasierte Untersuchung

“…[35]. In allen Fällen konnten die publizierten kinetischen Parameter [33][34][35] Desweiteren sind noch mit Borsäure funktionalisierte Harze erhältlich, die eine höhere Selektivität als die Ionentauschermaterialien aufweisen, aber dafür sehr stark durch kleine Beladungskapazitäten eingeschränkt sind [39]. Eine weitere Möglichkeit, Monosaccharide selektiv zu trennen, besteht in der Extraktion mit Borsäure oder anderen Carriern [16 -19].…”