2022
DOI: 10.1002/cbic.202200649
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The Ketosynthase Domain Controls Chain Length in Mushroom Oligocyclic Polyketide Synthases

Abstract: The nonreducing iterative type I polyketide synthases (NR-PKSs) CoPKS1 and CoPKS4 of the webcap mushroom Cortinarius odorifer share 88 % identical amino acids. CoPKS1 almost exclusively produces a tricyclic octaketide product, atrochrysone carboxylic acid, whereas CoPKS4 shows simultaneous hepta-and octaketide synthase activity and also produces the bicyclic heptaketide 6-hydroxymusizin. To identify the region(s) controlling chain length, four chimeric enzyme variants were constructed and assayed for activity … Show more

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Cited by 9 publications
(4 citation statements)
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“…However, when the SAT-KS-AT, or SAT-KS-AT-PT domains were swapped, the major compound was a C 18 polyketide, revealing that control of chain length resides within the KS domain 27 . KS domain swaps between NR-PKS CoPKS1, which synthesizes the octaketide atrochrysone carboxylic acid, and CoPKS4, which synthesizes both octaketides and heptaketides such as 6-hydroxymusizin, further corroborated the role of the KS domain in controlling polyketide chain length and identified ten amino acid residues that may be involved 28 .…”
Section: Polyketide Synthases (Pks)mentioning
confidence: 72%
“…However, when the SAT-KS-AT, or SAT-KS-AT-PT domains were swapped, the major compound was a C 18 polyketide, revealing that control of chain length resides within the KS domain 27 . KS domain swaps between NR-PKS CoPKS1, which synthesizes the octaketide atrochrysone carboxylic acid, and CoPKS4, which synthesizes both octaketides and heptaketides such as 6-hydroxymusizin, further corroborated the role of the KS domain in controlling polyketide chain length and identified ten amino acid residues that may be involved 28 .…”
Section: Polyketide Synthases (Pks)mentioning
confidence: 72%
“…Polyketide synthases are sensitive to even small changes in sequence [ 16 ], and close polyketide synthase homologs can produce different compounds [ 17 , 18 ]. The mutual orientation of the domains and the linkers between them was shown to be important for the product formation [ 18 , 19 ].…”
Section: Discussionmentioning
confidence: 99%
“…Polyketide synthases are sensitive to even small changes in sequence [ 16 ], and close polyketide synthase homologs can produce different compounds [ 17 , 18 ]. The mutual orientation of the domains and the linkers between them was shown to be important for the product formation [ 18 , 19 ]. The polyketide synthases selected for the present study differed in the linker sequences between the domains ( Supplementary Information S1 ), which may explain the differences in their ability to produce hispidin-like styrylpyrones.…”
Section: Discussionmentioning
confidence: 99%
“…The evidence of chain length controlled by KS has also been shown by a domain swap of the two closely related NR-iPKS CoPKS1 and CoPKS4, in which it is the KS domain that determines whether the dominant product is hepta-or octaketide. 46 The second perception comes from the bacterial type II HR-iPKS Iga11-Iga12 47 (Fig. 8B).…”
Section: B-ketosynthase (Ks) Domainsmentioning
confidence: 99%