The killing of mouse trophoblast cells by granulated metrial gland cells in vitro

Stewart, I. J.; Mukhtar, D. D. Y.

doi:10.1016/0143-4004(88)90054-9

Cited by 66 publications

(25 citation statements)

References 12 publications

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“…The lectin binding characteristics of cultured placental cells, when assessed in relation to the morphological groupings identified in a previous study (Stewart & Mukhtar, 1988) showed that there was no within group consistency in lectin binding. No placental group had a unique lectin binding characteristic.…”

Section: mentioning

confidence: 99%

“…There was a wide range of lectin binding to these cell populations with affinities ranging through galactose, mannose, N-acetylgalactosamine, N-acetylglucosamine and sialic acid residues, but not fucose. Previous studies have used morphological criteria to identify populations of cells isolated from the mouse placenta (Stewart & Mukhtar, 1988). Immunohisto- …”

Section: mentioning

confidence: 99%

“…Labyrinthine placental cells were prepared for cell culture as described previously (Stewart & Mukhtar, 1988). Briefly, uteri were removed under aseptic conditions and each uterine horn was opened by a midline incision along the antimesometrial uterine wall in Ca# + and Mg# + free Hanks' balanced salt solution (Flow Laboratories, Irvine, CA).…”

Section: Preparation Of Single Cell Suspensions Of Trophoblast Cellsmentioning

confidence: 99%

“…Apart from those of the giant cell trophoblast, distinguished by their large size, the individual cultured cell populations do not have sufficiently distinct mor- phological characteristics to determine their precise origin from within the fetal placenta (Stewart & Mukhtar, 1988). This may hamper, or prevent, the effective use of experimental procedures using isolated cell populations.…”

mentioning

confidence: 99%

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Lectin binding characteristics of mouse placental cells

2000

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The lectin binding characteristics of mouse placental cells were examined. Wax embedded tissue sections of placentae from d 14 pregnant mice were stained with 26 lectins, with a wide range of sugar specificities. Cell cultures prepared from d 14 mouse placentae and cultured for 24 h were stained with 7 of the lectins to determine if they could be used as markers for the different trophoblast cells in culture. In tissue sections all placental cell populations bound lectin but no lectin bound specifically to any single trophoblast population. All the lectins which bound to layer 1 cytotrophoblast lining the maternal blood spaces of the labyrinthine placenta also bound to the fetal endothelium of the labyrinthine placenta. Binding of lectin appeared strongest on the adluminal membrane of these cell populations suggesting a role for the carbohydrate moieties in nutrient transfer. Few lectins bound to junctional zone trophoblast. Overall, the binding of lectin to cultured cells did not correlate exactly with lectin binding to the cell populations in tissue sections. The value of lectins as markers for placental cells in culture was therefore found to be limited. Our findings indicate that carbohydrate expression by at least some placental cells may vary in culture from that expressed by the cells in vivo with obvious concerns for the validity of functional in vitro studies.

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Section: mentioning

confidence: 99%

Section: mentioning

confidence: 99%

Section: Preparation Of Single Cell Suspensions Of Trophoblast Cellsmentioning

confidence: 99%

mentioning

confidence: 99%

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Lectin binding characteristics of mouse placental cells

2000

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“…The function of perforin in this cell type is unknown. It has been postulated to assist in killing of stray trophoblast cells from the placenta (23) and in killing virus-infected cells (24), but there is at present little experimental evidence for either activity. Perforin-deficient mice reproduce normally and do not differ from WT mice in the morphology of the fetal-maternal interface.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Synthase-2 and Expression of Perforin in Uterine NK Cells

Burnett

Hunt

2000

The Journal of Immunology

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In human, mouse, and rat pregnancy, maternal NK cells accumulate and differentiate at implantation sites. These cells, termed uterine NK (uNK) cells, express NO synthase (NOS)-2 and develop cytolytic molecules such as perforin and granzymes during differentiation in situ. In this study, relationships between expression of the NOS-2 gene, uNK cell population density and tissue distribution, and synthesis of perforin were investigated. Uteri from wild-type (WT) and NOS-2−/− mice were collected at gestation days (g.d.) 8, 10, 12, 14, and 16 (n, >2/g.d.). Histochemical staining failed to reveal any differences between the population densities or tissue distributions of uNK cells in WT and NOS-2−/− uteri at any stage of gestation. By contrast, immunohistochemical staining with anti-perforin Abs demonstrated significantly fewer perforin-positive uNK cells in two uterine compartments of NOS-2−/− mice in comparison to the same compartments in WT mouse uteri. Perforin-positive uNK cells were reduced in NOS-2−/− metrial glands at g.d. 8, 10, and 12 and in decidua basalis at g.d. 12 (p < 0.05). Analysis of perforin protein by immunoblotting confirmed this observation. Northern blot hybridization studies showed that loss of perforin protein in NOS-2−/− mice was accompanied by decreased steady-state levels of perforin mRNA. These results demonstrate that migration of uNK cells into the uterus, selection of residency sites, and proliferation in situ are independent of NOS-2. By contrast, their differentiation, including transcription and translation of the cytotoxic molecule perforin, was shown to rely on normal expression of the NOS-2 gene.

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Hemopoietic origin of certain decidual cell precursors in murine pregnancy

et al. 1989

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The possible hemopoietic origin of certain precursors of uterine decidual cells appearing during normal murine pregnancy was investigated in semiallogeneic hemopoietic chimeras with retained or regained fertility. Chimeras were produced by three different methods in two donor-host combinations: F1 [BALB/c female x C3H/HeJ male] cells introduced into the parental strain BALB/c female hosts or F1 [CBA/J female x C57BL/6 male] cells introduced into CBA/J female hosts. Prenatal chimeras (PN) were made by reconstituting mouse fetuses (day 13-17) with 10(6)-10(7) adult bone marrow or fetal liver cells through the yolk sac and they were allowed to be delivered naturally. Neonatal chimeras (NN) were made by injecting 1-2 x 10(7) adult bone marrow cells into the anterior facial vein of neonatal mice (less than 24 hr old). In both cases, experimental animals were raised to maturity. Ovary-transplanted chimeras (OT) were made by injecting 10(7) bone marrow cells into lethally irradiated (9.5 Gy) young adult female mice, followed 6 weeks later with bilateral orthotopic transplants of syngeneic ovary grafts to restore fertility. All female chimeras produced by the three different methods were mated with syngeneic male partners to produce normal pregnancy. The extent of chimerism at the cellular level was determined in all cases by a radioautographic identification of the H-2 phenotype of splenic lymphocytes and decidual cells and macrophages in the collagenase-dispersed decidua at day 11-16 of normal pregnancy, following a sandwich labelling with monospecific anti-H-2 antibodies and 125I-protein A. Morphological discrimination of typical decidual cells from macrophages in the collagenase-dispersed decidua was carried out on the basis of several distinctive markers: presence of surface Dec-1 and Thy-1 and absence of surface F4/80 or latex phagocytosis for decidual cells, in contrast to macrophages which were phagocytic and expressed F4/80 but not Dec-1 or Thy-1. While the degree of hemopoietic chimerism (judged by the incidence of donor-derived lymphocytes in the spleen) varied from animal to animal, in all three groups (PN, NN, and OT) comprising a total of 26 chimeras, the percentage of typical decidual cells expressing donor H-2 phenotype showed an excellent correlation with that for small lymphocytes in the spleen. These results reveal that at least a subpopulation of typical decidual cells of the pregnant uterus has a hemopoietic genealogy. A possible familial relationship of these cells to granulated metrial gland cells remains unclear.

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The killing of mouse trophoblast cells by granulated metrial gland cells in vitro

Cited by 66 publications

References 12 publications

Lectin binding characteristics of mouse placental cells

Lectin binding characteristics of mouse placental cells

Nitric Oxide Synthase-2 and Expression of Perforin in Uterine NK Cells

Hemopoietic origin of certain decidual cell precursors in murine pregnancy

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