The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus

Cao, Yanghui; Xu, Liming; LaPatra, Scott E.; Zhao, Juanjuan; Liu, Miao; Liu, Hongbai; Lü, Tao; Zhang, Qi-Ya

doi:10.1016/j.molimm.2016.06.002

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…Injection with fish IFN plasmid alone can provide protection to some extent [ 32 ]. Our previous studies also confirmed antiviral activities of recombinant iIFN1a and IFN-γ2 proteins against IHNV, whereas protection decreases within 3 dpi [ 24 , 25 ]. Notably, the challenge was performed at 28 dpi.…”

Section: Discussionsupporting

confidence: 81%

“…In our study, cytokine plasmids further up-regulated expressions of antiviral proteins (Mx and viperin). Both our previous study and other's work proved that antiviral abilities of trout IFNs depend on dose and kinetics of antiviral state [ 24 , 40 ]. Hence, cytokine plasmids may enhance efficiency and longevity of nonspecific protection before the initial adaptive immune response takes effect.…”

Section: Discussionmentioning

confidence: 92%

“…NM_001164065.1, AJ279069.1, AJ555868.1, NM_001124619.2, NM_001124347.2, FJ184370.1, and FM864345.1), primers were designed to amplify their corresponding genes (Table 1 ). cDNA was prepared as PCR template according to our previous method [ 24 ]. PCR products were analyzed by agarose gel electrophoresis and purified using TIANgel Midi Purification Kit (Tiangen, China).…”

Section: Methodsmentioning

confidence: 99%

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Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model

Cao

Zhang

et al. 2017

Oncotarget

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Seven rainbow trout cytokine genes (interleukin (IL)-2, IL-8, IL-15, IL-17, IL-1β, intracellular interferon (iIFN) 1a, and IFN-γ2) were evaluated for their adjuvant effects on a DNA vaccine, called pG, containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV). Distinct DNA constructs in expression plasmid pcDNA3.1 encoding a cytokine gene were generated. Immunofluorescence assays in rainbow trout gonadal cells demonstrated successful protein expression from all these constructs. Subsequently, fish were immunized with pG alone or together with a cytokine expression plasmid. Results showed that each cytokine plasmids at an appropriate dose showed notable effects on immune gene expression. IL-17 and IFN-γ2 can enhance early specific IgM response. All cytokines, except IL-8, can benefit initial neutralizing antibody (NAb) titers. At 35 days post immunization (dpi), NAb titers of fish immunized with pG and IL-2, iIFN1a, or IFN-γ2 plasmids remained at high levels (1:160). NAb titers of fish immunized with pG alone decreased to 1:40. IL-8 or IL-1β can enhance antigen-specific proliferative T-cell responses at 14 dpi. At 28 dpi, coinjection of pG with IL-2, IL-8, IL-15, or IL-17 plasmids induced considerably stronger lymphocyte proliferation than that with injection of pG alone. All cytokine plasmids delivered with pG plasmid enhanced protection of trout against IHNV-mediated mortality. These results indicate that the type and dose of trout cytokine genes injected into fish affect quality of immune response to DNA vaccination.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model

Cao

Zhang

et al. 2017

Oncotarget

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“…qRT–PCR was performed with the One Step SYBR PrimeScript PLUS RT–PCR Kit (Perfect Real Time; Takara). The β-actin gene was used as the reference gene against which the gene expression levels were normalized 58 . The fold changes in gene expression were calculated relative to their expression in the mock-vaccinated control group (treated with pcDNA3.1 in PBS).…”

Section: Methodsmentioning

confidence: 99%

Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Zhao

Liu

et al. 2017

Sci Rep

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Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens of salmon and trout. An active bivalent DNA vaccine was constructed with the glycoprotein gene of Chinese IHNV isolate Sn1203 and VP2–VP3 gene of Chinese IPNV isolate ChRtm213. Rainbow trout (5 g) were vaccinated by intramuscular injection with 1.0 µg of the bivalent DNA vaccine and then challenged with an intraperitoneal injection of IHNV, IPNV, or both, at 30 and 60 days post-vaccination (d.p.v.). High protection rates against IHNV were observed, with 6% and 10% cumulative mortality, respectively, compared with 90–94% in the mock-vaccinated groups. IPNV loads (531-fold and 135-fold, respectively) were significantly reduced in the anterior kidneys of the vaccinated trout. Significant protection against co-infection with IHNV and IPNV was observed, with cumulative mortality rates of 6.67% and 3.33%, respectively, compared with 50.0% and 43.3%, respectively, in the mock-vaccinated groups. No detectable infective IHNV or IPNV was recovered from vaccinated trout co-infected with IHNV and IPNV. The bivalent DNA vaccine increased the expression of Mx-1 and IFN-γ at 4, 7, and 15 d.p.v, and IgM at 21 d.p.v., and induced high titres (≥160) of IHNV and IPNV neutralizing antibodies at 30 and 60 d.p.v.

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“…In addition to antiviral protection, interferon can also enhance adaptive immunity [5]. In our previous work, recombinant interferon proteins from rainbow trout were shown to elicit a significant antiviral response and provide efficient protection against IHNV [6]. The early protection provided by the vaccine is closely associated with the immediate IFN response [7].…”

Section: Introductionmentioning

confidence: 99%

Host miR-146a-3p Facilitates Replication of Infectious Hematopoietic Necrosis Virus by Targeting WNT3a and CCND1

Huang,

Zheng,

et al. 2024

Veterinary Sciences

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Infectious hematopoietic necrosis virus (IHNV) is a serious pathogen that causes great economic loss to the salmon and trout industry. Previous studies showed that IHNV alters the expression patterns of splenic microRNAs (miRNAs) in rainbow trout. Among the differentially expressed miRNAs, miRNA146a-3p was upregulated by IHNV. However, it is unclear how IHNV utilizes miRNA146a-3p to escape the immune response or promote viral replication. The present study suggested that one multiplicity of infection (MOI) of IHNV induced the most significant miR-146a-3p expression at 1 day post infection (dpi). The upregulation of miR-146a-3p by IHNV was due to viral N, P, M, and G proteins and relied on the interferon (IFN) signaling pathway. Further investigation revealed that Wingless-type MMTV integration site family 3a (WNT3a) and G1/S-specific cyclin-D1-like (CCND1) are the target genes of miRNA-146a-3p. The regulation of IHNV infection by miRNA-146a-3p is dependent on WNT3a and CCND1. MiRNA-146a-3p was required for the downregulation of WNT3a and CCND1 by IHNV. Moreover, we also found that WNT3a and CCND1 are novel proteins that induce the type-I IFN response in RTG-2 cells, and both of them could inhibit the replication of IHNV. Therefore, IHNV-induced upregulation of miRNA-146a-3p promotes early viral replication by suppressing the type-I IFN response by targeting WNT3a and CCND1. This work not only reveals the molecular mechanism of miRNA-146a-3p during IHNV infection but also provides new antiviral targets for IHNV.

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The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus

Cited by 11 publications

References 33 publications

Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model

Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model

Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Host miR-146a-3p Facilitates Replication of Infectious Hematopoietic Necrosis Virus by Targeting WNT3a and CCND1

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