The kinetics of ribulose-1,5-bisphosphate carboxylase/oxygenase in vivo inferred from measurements of photosynthesis in leaves of transgenic tobacco

Caemmerer, Susanne von; Evans, John R.; Hudson, Graham S.; Andrews, T. John

doi:10.1007/bf00206296

Cited by 360 publications

(286 citation statements)

References 36 publications

Supporting

Mentioning

271

Contrasting

Order By: Relevance

“…However, the wheat data are from two different experiments. Internal conductance per unit of exposed chloroplast surface area in young, fully expanded wheat leaves (Evans & Vellen, 1996) was similar to that of tobacco (Evans et al, 1994 ;see above). The hyperbolic relationship with chloroplast surface area indicates that increasing Rubisco content per unit leaf area is initially achieved by chloroplasts progressively covering more of the available exposed cell surface, but eventually when all the exposed surface is occupied, either the chloroplasts have to become thicker and\or the Rubisco concentration must increase.…”

Section: Relationship Between Internal Conductance and Rubiscomentioning

confidence: 57%

“…This can be seen for wheat leaves where Rubisco activity calculated from the slope of CO # response curves near the CO # compensation point is related to the extractable Rubisco content (Fig. 11) Caemmerer et al (1994). Curved line also includes the internal conductance shown in Fig.…”

Section: Relationship Between Internal Conductance and Rubiscomentioning

confidence: 86%

“…For a given Rubisco content, tobacco has greater internal conductance and more chloroplast surface area than wheat. The concentration of Rubisco in chloroplasts is also lower in tobacco (0.9-1.2 mM sites ; Evans et al, 1994) compared to wheat (1.5-2.2 mM sites ; Evans, 1983b). Fig.…”

Section: Relationship Between Internal Conductance and Rubiscomentioning

confidence: 98%

“…The extensive review of diffusion within leaves by Parkhurst (1994) emphasized the gas phase. Subsequent work combining gas exchange with other techniques has confirmed that diffusion through the liquid phase is generally more limiting than the gaseous phase within the leaf, with a crucial factor being the surface area of chloroplasts exposed to intercellular airspace per unit leaf area (Evans et al, 1994). The second objective of this paper is to examine the recent evidence concerning CO # diffusion within leaves with respect to leaf structure, and its impact on the performance of Rubisco.…”

Section: mentioning

confidence: 99%

“…The data have been separated into two groups : mesophytic leaves which are short-lived, herbaceous or deciduous species ; and sclerophytic leaves which either are evergreen or have a low specific leaf area. As the fluorescence method becomes unreliable when internal conductances exceed 0.3 mol m −# s −" , only data collected using the isotopic method were used Lloyd et al, 1992 ;Loreto et al, 1992 ;Evans et al, 1994 ;Epron et al, 1995 ;Syvertsen et al, 1995 ;Evans & Vellen, 1996 ;Roupsard et al, 1996 ;Lauteri et al, 1997. Solid lines from the origin indicate mean drawdown in CO # mole fraction (µmol mol −" ) across stomata (C a -C i ), or from substomatal cavities to the sites of carboxylation (C i -C c ).…”

Section: Restrictions To Comentioning

confidence: 99%

See 4 more Smart Citations

Leaf anatomy enables more equal access to light and CO₂ between chloroplasts

1999

Self Cite

View full text Add to dashboard Cite

The function of a leaf is photosynthesis, which requires the interception of light and access to atmospheric CO # while controlling water loss. This paper examines the influence of leaf anatomy on both light capture and CO # diffusion. As photosynthetic metabolism is spread between many chloroplasts, a leaf faces the challenge of matching light capture by a given chloroplast with the metabolic capacity of that chloroplast. Chloroplasts nearest the leaf surface receive the greatest irradiance and therefore absorb more light per unit chlorophyll than chloroplasts in the centre of a leaf. Electron transport and carbon fixation capacities per unit of chlorophyll decline with increasing depth in the leaf, to compensate for the decline in light absorbed per unit chlorophyll. Many key photosynthetic protein complexes in chloroplasts have nuclear encoded genetic information. Consequently, all chloroplasts within a given cell have a similar metabolic complement, which limits the potential gradient of photosynthetic capacity per unit chlorophyll across the leaf. A simple model couples light absorption through the leaf (based on the Beer-Lambert law) with the profile of chlorophyll through a leaf and the gradient in photosynthetic capacity. It is validated by comparison with "%CO # fixation profiles through spinach leaves obtained in various studies. The model can account for published "%C fixation profiles obtained with blue, red and green light of different irradiances and white light applied in different combinations to the adaxial and abaxial surfaces of spinach leaves. The model confirms that spongy mesophyll increases the apparent extinction coefficient of chlorophyll compared to palisade tissue. The palisade tissue nearest the surface which receives light facilitates the penetration of light to a greater depth, while spongy mesophyll promotes scattering to enhance light absorption, thus reducing the gradient in light absorbed per unit chlorophyll through a leaf. CO # fixation faces a diffusional limitation, which necessitates Rubisco to be spread evenly across the cell walls exposed to intercellular airspace. Mesophyll cell structure reflects the need to have a large cell surface per unit volume exposed to airspaces. The regular array of columnar cells in palisade tissue, or cell lobing in monocot leaves, results in greater exposed surface per unit tissue volume than spongy mesophyll. The exposed surface area per unit leaf area scales with photosynthetic capacity such that the difference in CO # partial pressure between substomatal cavities and the sites of carboxylation within chloroplasts is, on average, independent of photosynthetic capacity of the leaf. However, Rubisco specific activity declines as the Rubisco content per unit leaf area increases due to greater internal diffusional limitations.

show abstract

Section: Relationship Between Internal Conductance and Rubiscomentioning

confidence: 57%

Section: Relationship Between Internal Conductance and Rubiscomentioning

confidence: 86%

Section: Relationship Between Internal Conductance and Rubiscomentioning

confidence: 98%

Section: mentioning

confidence: 99%

Section: Restrictions To Comentioning

confidence: 99%

See 3 more Smart Citations

Leaf anatomy enables more equal access to light and CO₂ between chloroplasts

1999

Self Cite

View full text Add to dashboard Cite

show abstract

Manipulating Ribulose Bisphosphate Carboxylase/Oxygenase in the Chloroplasts of Higher Plants

Andrews¹,

Whitney²

2005

Artificial Photosynthesis

Self Cite

View full text Add to dashboard Cite

Photosynthetic parameters and stomatal conductance in attached and detached balsam fir foliage

Akalusi

Meng

Bourque

2021

Plant Enviro Interactions

View full text Add to dashboard Cite

Leaf level gas‐exchange measurements can be made on detached foliage to address the challenge of access to the crown of tall trees. However, detachment may impact leaf gas exchange. This necessitates the study of gas‐exchange characteristics of foliage on detached branches to assess the feasibility of using detached branches for gas‐exchange analysis. We compared photosynthetic parameters and stomatal conductance in foliage of attached and detached branches of balsam fir [Abies balsamea (L.) Mill.] during the growing season. Data were analyzed using a linear mixed‐effect model, with fixed and random effects (branch status and measurement month, and tree number, respectively). Branch detachment had no significant effects on: (i) photosynthesis at the current ambient CO2 concentration (400 µmol mol−1, A400); (ii) maximum rates of Ribulose‐1,5‐bisphosphate (RuBP) carboxylation (Vcmax) and regeneration (Jmax); (iii) the ratio of Jmax to Vcmax (i.e., Jmax:Vcmax), and (iv) stomatal conductance (gs) during the study period (p = 0.120–0.335). There was a strong seasonal effect on all gas‐exchange variables (p ≤ 0.001–0.015). Gas‐exchange measurements made on detached foliage during the warm summer months should be performed with care. Reliable gas‐exchange measurements can be obtained using balsam fir foliage on detached branches 50–80 cm in length, in cooler growing‐season months, up to 30 min after detachment.

show abstract

The kinetics of ribulose-1,5-bisphosphate carboxylase/oxygenase in vivo inferred from measurements of photosynthesis in leaves of transgenic tobacco

Cited by 360 publications

References 36 publications

Leaf anatomy enables more equal access to light and CO₂ between chloroplasts

Leaf anatomy enables more equal access to light and CO₂ between chloroplasts

Manipulating Ribulose Bisphosphate Carboxylase/Oxygenase in the Chloroplasts of Higher Plants

Photosynthetic parameters and stomatal conductance in attached and detached balsam fir foliage

Contact Info

Product

Resources

About

The kinetics of ribulose-1,5-bisphosphate carboxylase/oxygenase in vivo inferred from measurements of photosynthesis in leaves of transgenic tobacco

Cited by 360 publications

References 36 publications

Leaf anatomy enables more equal access to light and CO2 between chloroplasts

Leaf anatomy enables more equal access to light and CO2 between chloroplasts

Manipulating Ribulose Bisphosphate Carboxylase/Oxygenase in the Chloroplasts of Higher Plants

Photosynthetic parameters and stomatal conductance in attached and detached balsam fir foliage

Contact Info

Product

Resources

About

Leaf anatomy enables more equal access to light and CO₂ between chloroplasts

Leaf anatomy enables more equal access to light and CO₂ between chloroplasts