The late results of intravenous injection of colloidal iron

Cappell, D. F.

doi:10.1002/path.1700330115

Cited by 70 publications

(22 citation statements)

References 5 publications

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“…These inclusions reflect the severe systemic siderosis produced by absorption of the major part of the 10 mg. iron administered as iron-dextran, a dose equivalent to five times the total normal body iron of the mouse. Nuclear iron-containing bodies are described by Lauda and Haam [1925], Cappell [1930], Nissim [1953], Haddow [1959] and are illustrated in a recent paper by Haddow and Horning [1960] (fig. 21).…”

Section: Identification Ofmentioning

confidence: 99%

Observations on Subcutaneous Macrophages. Phagocytosis of Iron‐dextran and Ferritin Synthesis

Muir

Golberg

1961

Exp Physiol

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The structure of macrophages at the site of subcutaneous injections of iron-dextran has been studied with the electron microscope. Iron-dextran has a macromolecular structure which can be distinguished from that of its metabolic product, the iron-storage protein ferritin. By a process of pinocytosis the macrophages ingest iron-dextran, then concentrate it within cytoplasmic vacuoles, apoferritin is synthesized and the iron is finally stored within this protein to form ferritin. At first individual molecules of ferritin are uniformly distributed throughout the cytoplasm, accompanied by a few molecules in the nucleoplasm but, as a rule, none in mitochondria. One week after injection, vacuoles containing a mixture of iron-dextran and ferritin are found, and these are gradually replaced by vacuoles containing only ferritin.COLLOIDAL iron preparations contain macromolecular particles which can be distinguished in electron micrographs from the images produced by the ferric hydroxide micelles of the iron-storage protein, ferritin [Richter, 1959;Bessis and Breton-Gorius, 1959;Muir, 1960]. Thus it is possible to observe the cellular transformation of administered iron preparations into ferritin and haemosiderin. After intraperitoneal injections of iron-dextran, saccharated iron oxide and hydrous ferric oxide in the mouse, Richter [1959] studied, with high resolution electron microscopy and electron diffraction, the incorporation of the injected material in hepatic and splenic macrophages. Some of these experiments have been repeated and the results confirmed by Bessis and Breton-Gorius [1959].In the inevitably small samples of tissue examined in the electron microscope, such studies of the synthesis of ferritin in the splenic macrophages could be confused by the random association of pre-existing ferritin with the injected colloidal iron compounds. It is preferable to work with tissues at a subcutaneous injection site, which normally does not contain ferritin; here it can be assumed that any ferritin synthesized stores the administered iron. Richter [1959] mentions briefly the subcutaneous changes 1-16 days after injection of hydrous ferric oxide, illustrating only a cytoplasmic iron depot at 5 days. The fate of colloidal iron and isolated haemoglobin injected subcutaneously in the mouse is reported bv Wessel and Gedigk [1959]. In the present study attention is given to the method of ingestion of irondextran by macrophages and the subsequent synthesis of ferritin in their cytoplasm.

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Section: Identification Ofmentioning

confidence: 99%

Observations on Subcutaneous Macrophages. Phagocytosis of Iron‐dextran and Ferritin Synthesis

Muir

Golberg

1961

Exp Physiol

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“…Witts (1) A great deal of work has been done upon the deposition and excretion of injected iron. It may be stored in animals mostly in the liver and spleen (24), (25), (26). It is excreted by the bowel (27) and by the kidneys (17), (28 (17) found that in cases of chlorosis which were given iron subcutaneously, iron in increased amounts was found in the urine invariably, and therefore he believed that all the iron was not utilized in the formation of new hemoglobin.…”

Section: Commentmentioning

confidence: 99%

Quantitative Aspects of Iron Deficiency in Hypochromic Anemia

Heath¹,

Strauss²,

Castle³

1932

J. Clin. Invest.

113

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“…Readily discernible effects of tissue storage iron are seen only under the added stress of a complementary insult, or, as appears to be the case in certain human diseases, when a critical level of intraceUular iron is reached and maintained. It is probable that this character of iron-induced liver damage is responsible for the numerous failures to create hemochromatosis in the laboratory animal (21), 22).…”

Section: Importance Of Findings To Iron Storage Diseases--the Resultmentioning

confidence: 99%

A Study of Iron-Induced Liver Damage

Witzleben

Chaffey

1962

The Journal of Experimental Medicine

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The role of iron storage in the liver damage in the various conditions where cirrhosis and massive siderosis coexist is as yet unsettled, despite considerable accumulating evidence that such storage plays a critical role in the pathogenesis of these lesions (1, 2).The possibility that different hepatic insults m a y potentiate or augment their individual effects, with a resultant damage greater than would have resulted from either alone, is often considered as a possible explanation for hepatic diseases of obscure origin. The validity of this concept, however, has seldom been critically investigated (3).These two problems have been studied b y means of examining the histochemistry of the iron-loaded liver and by determining the effect of iron loading on the response of the liver to a series of hepatic toxins whose mechanisms of toxicity are quite well known. Materials and MethodsMice of the Great Ormond Street strain were used. At the beginning of the injection period, which lasted approximately 3 weeks, the animals were 2 to 3 months of age. Iron was administered as iron-dextran (imferon) in divided doses to a total dose of 1000 mg/kg. Carbon tetraehioride was administered in a single dose of 0.01 mi in olive oil subcutaneously. Thioacetamide was administered in one dose of 200 mg/kg subcutaneously. Bromobenzeue was administered in a single dose of 0.05 ml/100 gm body weight in olive oil after 12 hours. Intervals of sacrifice varied with toxin administered. Only among the animals given bromobenzeue did spontaneous deaths occur. Animals were killed by cervical dislocation, and slices of liver were fixed in 10 per cent buffered formalin, Regand's fluid, and Lillie's acetic alcohol formalin fixafive. Other slices were frozen at --76°C for histochemical and chemical studies. The stains employed included hematoxylln and eosin, Regaud's method for mitochondria, Giemsa for cytoplasmic basophilic substance (RNA), Perls' reaction for stainable iron, periodic acidSchiff (PAS) reaction before and after digestion with saliva, Best's carmine for glycogen, and Peaxse's modification of the Ziehi-Neelsen stain (4). Enzyme histochemical procedures were those of Pearse for succinie dehydrogenase using 3-(4,5 dimethyl-thiazolyl-2).2, S diphenyl tetrazolium bromide (4), Scarpelli et al. for DPN and TPN diphorase (S), and Chiquoine (6) for glucose-6-phosphatase. Glucose-6-phosphatase was determined chemically by a modified method of Swanson (7).

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The late results of intravenous injection of colloidal iron

Cited by 70 publications

References 5 publications

Observations on Subcutaneous Macrophages. Phagocytosis of Iron‐dextran and Ferritin Synthesis

Observations on Subcutaneous Macrophages. Phagocytosis of Iron‐dextran and Ferritin Synthesis

Quantitative Aspects of Iron Deficiency in Hypochromic Anemia

A Study of Iron-Induced Liver Damage

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