The lateral motion of lipid molecules in the apical plasma membrane of endothelial cells is reversibly affected by the presence of cell junctions

Tournier, Jean Francois; Lopez, André; Gas, Nicole; Tocanne, Jean‐François

doi:10.1016/0014-4827(89)90095-5

Cited by 20 publications

(5 citation statements)

References 28 publications

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“…At the body temperature of mammals, the hydrophobic moiety of membrane lipids is in a liquid state, and in spite of strong hydrophilic bonds between polar groups and between the surrounding water, molecules can diffuse in the plane of the membrane. However, some lipid species are preferentially confined to a given membrane domain (32,115,(121)(122)(123)(124)127). Lipid probes such as 5-[N-hexadecanoyl] amino fluorescein, 1-acyl-2-[N-4-nitrobenz-2-oxa-1, 3-diazole]aminocaproyl phosphatidylcholine, and gangliosides added to the apical membrane are unable to pass through the TJ.…”

Section: Polarized Distribution Of Membrane Molecules That Depend On Tjsmentioning

confidence: 99%

Role of Tight Junctions in Establishing and Maintaining Cell Polarity

Cereijido

Valdés

Shoshani

et al. 1998

Annu. Rev. Physiol.

239

138

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The tight junction (TJ) is not randomly located on the cell membrane, but occupies a precise position at the outermost edge of the intercellular space and, therefore, is itself considered a polarized structure. This article reviews the most common experimental approaches for studying this relationship. We then discuss three main topics. (a) The mechanisms of polarization that operate regardless of the presence of TJs: We explore a variety of polarization mechanisms that operate at stages of the cell cycle in which TJs may be already established. (b) TJs and polarity as partners in highly dynamic processes: Polarity and TJs are steady state situations that may be drastically changed by a variety of signaling events. (c) Polarized distribution of membrane molecules that depend on TJs: This refers to molecules (mainly lipids) whose polarized distribution, although not the direct result of TJs, depends on these structures to maintain such distribution.

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Section: Polarized Distribution Of Membrane Molecules That Depend On Tjsmentioning

confidence: 99%

Role of Tight Junctions in Establishing and Maintaining Cell Polarity

Cereijido

Valdés

Shoshani

et al. 1998

Annu. Rev. Physiol.

239

138

View full text Add to dashboard Cite

show abstract

“…Recently, new techniques such as "single particle tracking" demonstrate that lateral diffusion is not homogeneous throughout the plasma membrane of most cells (Anderson et al, 1992;Cherry et al, 1994;Jacobson et al, 1995). Specific examples include cell junctions of vascular endothelium (Tournier et al, 1989) and neurons (Joe and Angelides, 1993).…”

Section: Introductionmentioning

confidence: 99%

Lateral diffusion in planar lipid bilayers: a fluorescence recovery after photobleaching investigation of its modulation by lipid composition, cholesterol, or alamethicin content and divalent cations

Ladha¹,

Mackie²,

Harvey³

et al. 1996

Biophysical Journal

120

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In spite of the fact that planar lipid bilayers are still the best-suited artificial membrane system for the study of reconstituted ion channels and receptors, data dealing with their physical characterization, especially as regards dynamics, are scanty. A combined electrical and optical chamber was designed and allowed fluorescence recovery after photobleaching recovery curves to be recorded from stable virtually solvent-free bilayers. D, the lateral diffusion coefficient of N-(7-nitrobenzoyl-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn- glycero-3-phosphoethanolamine, was found to be relatively insensitive to the phospholipid composition (headgroup, chain unsaturation, etc.), whereas inclusion of 33-50% cholesterol in the membrane reduced D by a factor of 2. Divalent cations significantly reduced D of negatively charged bilayers. These results compare well with data gathered on other model and natural systems. In addition, the incorporation of the voltage-dependent pore-former alamethicin did slightly reduce lipid lateral mobility. This study demonstrates the feasibility of such experiments with planar bilayers, which are amenable to physical constraints, and thus offers new opportunities for systematic studies of structure-function relationships in membrane-associating molecules.

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“…Due to its lipophilic character the alkyl tail of this probe inserts into the membrane of the colonocytes. Hitherto, HAF had been used for investigations of lateral diffusion of lipids in biomembranes (Dragsten et al 1981;Dupou et al 1987;Tournier et al 1989) and for measurements of resonance energy transfer in lipid membranes (Remmers & Neubig, 1993). The localization of the dye molecule at the epithelial surface was demonstrated by means of a confocal laser-scanning microscope and by using IgG antibodies.…”

mentioning

confidence: 99%