The lectin accumulation, growth rate, carrageenan yield, and quality from the red alga Kappaphycus striatus cultivated at Camranh Bay, Vietnam

Huong²

2020

Vietnam J. Biotechnol.

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The red alga Kappaphycus striatum is an economically important species and extensively cultivated in Vietnam as a material source for carrageenan production. To evaluate carrageenan quality, the characterization of carrageenan extracted from this alga was investigated. As a result, chemical composition of carrageenan consists of 32.4% of 3,6 anhydrogalactose and 24.3% of sulfate. Gelling and metling temperatures are 34.4oC and 55.6oC, respectively. Gel strength of 1.5% is 615 g/cm2 and average molecular weight is about 267 kDa. Furthermore, FT-IR spectrum showed intense absorption bands at 930 cm-1 and 850 cm-1 that attributed to 1,4-linked 3,6 anhydro-α-D-galactose and 1,3-linked β-D-galactose-4-sulfate of kappa-carrageenan, respectively. 13C NMR spectrum indicated the signals for anomeric carbon of β-D-galactose-4-sulfate at 102.6 ppm and anomeric carbon of 3,6-anhydro-α-D-galactose at 95.3 ppm. 13H NMR spectrum showed peak signals at 3.57 ppm and 5.1 ppm that corresponds with O-methyl proton of 1,3-linked 6-O-methyl-D-galactose and α-anomeric proton of 3,6 anhydro-α-D-galactose residues, respectively. The results show that the carrageenan from the red alga Kappaphycus striatus is kappa-carrageenan with the repeating disaccharide unit consisting of 1,3-linked 6-O-methylated, β-D-galactose-4-sulfate and 1,4-linked 3,6 anhydro-α-D-galactose and did not contain iota-carrageenan. Therefore, this alga may promise to be a good source for carrageenan production for application in food or medicine.

Section: Carrageenans Possess Various Pharmaceuticalmentioning

confidence: 99%

Characterization of kappa-carageenan from the red alga Kappaphycus striatum

Huong²

2020

Vietnam J. Biotechnol.

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“…In Vietnam, the red alga Kappaphycus striatus has been cultivated commercially since 2005, due to transport of algal seeds from Bohol, Philippines [15]. In this note, we cloned the cDNA encoding a new lectin KSL and elucidated the primary structure as anticancer activity on five carcinoma cell lines, which may provide valuable information for application as functional food lectin(s).…”

Section: Introductionmentioning

confidence: 99%

Structure and anticancer activity of a new lectin from the cultivated red alga, Kappaphycus striatus

Trinh

2020

J Nat Med

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The red alga Kappaphycus striatus is economically important food species and extensively cultivated throughout most tropical parts of the world as a source of carrageenan. In this note, the primary structure of a new lectin KSL from this alga was elucidated by the rapid amplification method of complementary DNA (cDNA) ends, which consists of 267 amino acid residues distributed in four tandem-repeated domains of about 67 amino acids and sharing 43% of identity. The calculated molecular mass from the deduced sequence was consistent with that of natural KSL (27,826 Da) determined by electron spray ionization-mass spectrometry. The primary structure of KSL showed high similarity to those of the high mannose N-glycan specific lectins from marine red algae, ESA-2 from Eucheuma serra, EDA-2 from Eucheuma denticulatum, KSA-2 from Kappaphycus striatum, KAAs from Kappaphycus alvarezii and SfLs from Solieria filiformis, and from microorganisms, BOA from Burkholderia oklahomensis, MBHA from Myxococcus xanthus, OAA from Oscillatoria agardhii and PFL from Pseudomonas fluorescens. Furthermore, KSL showed anticancer effects against five carcinoma cell lines, HT29, Hela, MCF-7, SK-LU-1 and AGS, in a dose-dependent manner with the IC 50 values of 0.80-1.94 µM, whereas its inhibition activities on cancer cells were not detected in the presence of yeast mannan, an inhibitor against lectin KSL. The cultivated red alga K. striatus could also be a good source of functional lectin(s) for application as anticancer agents.

“…These species may be potential sources of biologically active compounds including lectins. However, very little information is known concerning lectins from Vietnamese marine organisms, except several reports on the screening results of hemagglutinins from Vietnamese marine algae and invertebrates (Le Dinh Hung et al, 2009aHung et al, , 2012Dinh Thanh Trung et al, 2017), the purification and characterization of lectins from the red algae K. alvarezii, K. striatum, E. denticulatum, Gracilaria salicornia, Hydropuntia eucheumatoides and from marine sponge Stylissa flexibilis (Le Dinh Hung et al, 2009bHung et al, , 2011Hung et al, , 2013Hung et al, , 2015aHung et al, , 2018aHung et al, , 2018b, the cDNA clones encoding lectins from K. striatum and E. denticulatum (Le Dinh Hung et al, 2015a, 2015b and seasonal variations in lectin contents from the cultivated red algae K. alvarezii and K. striatus (Le Dinh Hung et al, 2009c, 2019. Thus, the objective of present research was to report on the isolation, biochemical properties and high mannose N-glycan binding specificity of a new lectin from the red alga Betaphycus gelatinus, which can provide valuable information regarding lectins from the carrageenophyte algal family.…”

Section: Introductionmentioning

confidence: 99%

High-mannose type N-glycan binding specificity of a novel lectin from the red alga (Betaphycus gelatinus)

Thuc²

2020

Vietnam J. Biotechnol.

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The red alga, Betaphycus gelatinus is one of carrageenan sources in the world. The lectin from the red alga B. gelatinus was isolated by a combination of aqueous ethanol extraction, ethanol precipitation, ion-exchange chromatography and gel filtration chromatography and was designated as BGL after the specific name of alga. Lectin gave a single band with molecular mass of about 19,000 Da in both non-reducing and reducing SDS-PAGE conditions, therefore lectin exists in monomer form. The hemagglutination activities of BGL were stable over a wide range of pH from 3 to 10, temperature up 60 oC and not affected by either the presence of EDTA or addition of divalent cations, indicating that lectin requires no metal for biological activity. The hemagglutination activities of BGL were not inhibited by monosaccharides and glycoproteins, D-glucose, D-mannose, D-galactose, D-xylose, N-acetyl-galactosamine, N-acetyl-glucosamine, N-acetyl-neuraminic acid, N-acety-D-mannosamine, transferrin and fetuin, but strongly inhibited by glycoproteins bearing high-mannose type N-glycan, such as yeast mannan and porcine thyroglobulin. Lectin BGL is specific for N-glycans and may recognize terminal (α1–3) or (α1–6)-linked mannose residues in structure Man(α1-6)[Man(α1-3)]Man(α1-6)[Man(α1-3)]Man(β1-4)GlcNAc(β1-4)GlcNAc of N-glycans. High-mannose type N-glycan binding specificities of this lectin highly resemble with those of the anti-cancer, anti-virus and anti-bacteria lectins from the red algae, carrageenophytes, including Eucheuma serra (ESA-2), Eucheuma denticulatum (EDA-2), Kappaphycus striatum (KSA-2), Kappaphycus alvarezii (KAA-1 and KAA-2) and Solieria filiformis (SfL1 and SfL2). The red alga B. gelatinus could promise to be a good source of valuable lectins for application in biochemistry and biomedicine.