The legacy of mRNA engineering: A lineup of pioneers for the Nobel Prize

Janowski, Mirosław; Andrzejewska, A

doi:10.1016/j.omtn.2022.07.003

Cited by 10 publications

(3 citation statements)

References 155 publications

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“…Vaccines based on antigen delivery through mRNA are produced by a simple and fast procedure which consists of the amplification of the RNA nucleotide sequence encoding the open reading frame of the desired gene, plus further modifications to enhance stability and translation. The procedure is performed in automatized factories in which the risk of contamination with unrelated material is very low [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

mRNA Vaccines against SARS-CoV-2: Advantages and Caveats

Echaide

Chocarro

Bocanegra

et al. 2023

IJMS

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The application of BNT162b2 and mRNA-1273 vaccines against SARS-CoV-2 infection has constituted a determinant resource to control the COVID-19 pandemic. Since the beginning of 2021, millions of doses have been administered in several countries of North and South America and Europe. Many studies have confirmed the efficacy of these vaccines in a wide range of ages and in vulnerable groups of people against COVID-19. Nevertheless, the emergence and selection of new variants have led to a progressive decay in vaccine efficacy. Pfizer–BioNTech and Moderna developed updated bivalent vaccines—Comirnaty and Spikevax—to improve responses against the SARS-CoV-2 Omicron variants. Frequent booster doses with monovalent or bivalent mRNA vaccines, the emergence of some rare but serious adverse events and the activation of T-helper 17 responses suggest the need for improved mRNA vaccine formulations or the use of other types of vaccines. In this review, we discuss the advantages and limitations of mRNA vaccines targeting SARS-CoV-2 focusing on the most recent, related publications.

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Section: Introductionmentioning

confidence: 99%

mRNA Vaccines against SARS-CoV-2: Advantages and Caveats

Echaide

Chocarro

Bocanegra

et al. 2023

IJMS

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“…The huge potential of nucleotides for developing advanced therapies has resulted in a very fruitful field of research. Among the most relevant therapeutic applications, there could be highlighted the following: (1) gene transfection with plasmids [ 30 , 31 ], (2) development of vaccines and protein modulation therapies employing miRNAs [ 32 , 33 ], (3) gene silencing therapies [ 34 , 35 ], and (4) the most recent to arrive, CRISPR genome editing [ 36 , 37 ]. However, like with many other therapeutic biomolecules, the indiscriminate use of nucleotides is not easy and immediate, as they suffer from quick degradation in extracellular regions and intracellular environments and are not able to cross biological membranes.…”

Section: Development Of Silica-based Nanomedicines Using Therapeutic ...mentioning

confidence: 99%

Delivery of Therapeutic Biopolymers Employing Silica-Based Nanosystems

2023

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The use of nanoparticles is crucial for the development of a new generation of nanodevices for clinical applications. Silica-based nanoparticles can be tailored with a wide range of functional biopolymers with unique physicochemical properties thus providing several advantages: (1) limitation of interparticle interaction, (2) preservation of cargo and particle integrity, (3) reduction of immune response, (4) additional therapeutic effects and (5) cell targeting. Therefore, the engineering of advanced functional coatings is of utmost importance to enhance the biocompatibility of existing biomaterials. Herein we will focus on the most recent advances reported on the delivery and therapeutic use of silica-based nanoparticles containing biopolymers (proteins, nucleotides, and polysaccharides) with proven biological effects.

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“…mRNA-based cell modification is an integration-free method that can activate immune cells’ response, restore cells’ physiological functions, and even provide them with new functionalities. 6 In MSC-related research, mRNA has been used to stimulate their reprogramming, e.g., a cell with cardiac-like phenotype, 7 or to enhance its delivery to the target organ, as shown for CXCR2 mRNA-engineered MSC transplantation in a mouse model of inflammatory bowel disease. 8 In our previous research, we used mRNA-ITGA4 to induce the overexpression of integrin-α4 in MSCs.…”

Section: Introductionmentioning

confidence: 99%