The let-7g microRNA promotes follicular granulosa cell apoptosis by targeting transforming growth factor-β type 1 receptor

Zhou, Jilong; Liu, Jiying; Pan, Zengxiang; Du, Xiaohong; Li, Xinyu; Ma, Baiquan; Wang, Yao; Li, Qifa; Liu, Honglin

doi:10.1016/j.mce.2015.03.012

Cited by 59 publications

(45 citation statements)

References 56 publications

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“…Much of the existing evidence has been obtained using follicular cell cultures, mostly granulosa cells. Often reported effects of miRNAs include either the promotion or suppression of granulosa cell apoptosis [5], [6], [7], [8], [9], [10]. Yet, in many cases, the site of expression, if any, of these miRNAs within follicles (ie, granulosa and theca compartments) or whether their expression actually changes during follicle atresia, supporting their physiological role, has not been clarified.…”

Section: Introductionmentioning

confidence: 99%

A miRNA target network putatively involved in follicular atresia

Donadeu

Mohammed

Ioannidis

2017

Domestic Animal Endocrinology

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In a previous microarray study, we identified a subset of micro RNAS (miRNAs), which expression was distinctly higher in atretic than healthy follicles of cattle. In the present study, we investigated the involvement of those miRNAs in granulosa and theca cells during atresia. Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR) confirmed that miR-21-5p/-3p, miR-150, miR-409a, miR-142-5p, miR-378, miR-222, miR-155, and miR-199a-5p were expressed at higher levels in atretic than healthy follicles (9–17 mm, classified based on steroidogenic capacity). All miRNAs except miR-21-3p and miR-378 were expressed at higher levels in theca than granulosa cells. The expression of 13 predicted miRNA targets was determined in follicular cells by RT-qPCR, revealing downregulation of HIF1A, ETS1, JAG1, VEGFA, and MSH2 in either or both cell types during atresia. Based on increases in miRNA levels simultaneous with decreases in target levels in follicular cells, several predicted miRNA target interactions were confirmed that are putatively involved in follicular atresia, namely miR-199a-5p/miR-155-HIF1A in granulosa cells, miR-155/miR-222-ETS1 in theca cells, miR-199a-5p-JAG1 in theca cells, miR-199a-5p/miR-150/miR-378-VEGFA in granulosa and theca cells, and miR-155-MSH2 in theca cells. These results offer novel insight on the involvement of miRNAs in follicle development by identifying a miRNA target network that is putatively involved in follicle atresia.

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Section: Introductionmentioning

confidence: 99%

A miRNA target network putatively involved in follicular atresia

Donadeu

Mohammed

Ioannidis

2017

Domestic Animal Endocrinology

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“…Concomitant with their role, they are known to regulate target genes which are responsible in biological events such as GC apoptosis [50], proliferation [63,64], and pluripotency [65,66]. Let-7g, one of the members of the let-7 family, appears to play a significant role in follicular atresia and other diverse biological processes (for example, angiogenesis, proliferation and differentiation).…”

Section: Functional Roles Of Mirnas During Follicular Cellular Apomentioning

confidence: 99%

“…Recently, it has become clear that a number of miRNAs control follicular atresia through their target genes and different signaling pathways, thereby modulating GC apoptosis [48,49,50,51,52,53]. This work provides new insights to further understand the mechanism of follicular atresia.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs: New Insight in Modulating Follicular Atresia: A Review

Worku

Rehman

Talpur

et al. 2017

IJMS

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Our understanding of the post-transcriptional mechanisms involved in follicular atresia is limited; however, an important development has been made in understanding the biological regulatory networks responsible for mediating follicular atresia. MicroRNAs have come to be seen as a key regulatory actor in determining cell fate in a wide range of tissues in normal and pathological processes. Profiling studies of miRNAs during follicular atresia and development have identified several putative miRNAs enriched in apoptosis signaling pathways. Subsequent in vitro and/or in vivo studies of granulosa cells have elucidated the functional role of some miRNAs along with their molecular pathways. In particular, the regulatory roles of some miRNAs have been consistently observed during studies of follicular cellular apoptosis. Continued work should gradually lead to better understanding of the role of miRNAs in this field. Ultimately, we expect this understanding will have substantial benefits for fertility management at both the in vivo or/and in vitro levels. The stable nature of miRNA holds remarkable promise in clinical use as a diagnostic tool and in reproductive medicine to solve the ever-increasing fertility problem. In this review, we summarize current knowledge of the involvement of miRNAs in follicular atresia, discuss the challenges for further work and pinpoint areas for future research.

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“…In the same study, in vivo anti-miR-21 treated ovary resulted in reduced rate of ovulation compared to the untreated contralateral ovary (Carletti et al, 2010). The let-7g induces apoptosis in pig granulosa cells by targeting the TGFBR1 gene (Zhou et al, 2015), while porcine atretic follicles are highly enriched with let-7g and its overexpression promoted apoptosis of granulosa cells (Cao et al, 2015). MiR-26b promotes porcine granulosa cells apoptosis and induces follicular atresia by targeting SMAD4 (Liu et al, 2014) and ATM (Lin et al, 2012).…”

Section: Potential Role Of Mirnas In Controlling Ovarian Functionsmentioning

confidence: 99%

Dynamics and role of MicroRNAs during mammalian follicular development

Gebremedhn¹,

Pandey²,

Salilew‐Wondim³

et al. 2016

Anim Reprod

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Ovarian functions, which involve dynamically regulated processes of selection, recruitment and dominance, are known to be regulated by an array of genes, which are expressed in spatiotemporal manner in follicular somatic cells and gametes. This differential expression of genes in mammalian follicular cells is partly regulated by posttranscriptional gene regulators named microRNAs (miRNAs). In addition to the cellular miRNAs, growing number of evidences are available on the potential role of extracellular vesicles mediated transfer of miRNAs in follicular fluid. These extracellular vesicles are shown to be involved in cellto-cell communication within the follicular environment. The molecular messages carried by the extracellular vesicles released into extracellular space are thought to be reflections of the physiological status of the cells from where they are released. Therefore due to their structural nature and potential to cargo several physiologically relevant molecules, exosomes have a great potential to be used as markers of oocyte developmental competence in follicular environment. Here, we review large sets of literatures to show the dynamic nature of miRNAs during various stages of mammalian follicular development, oocyte growth and the role of some of the miRNAs in ovarian cell functions. Moreover, the presence of microvesicle and exosome-coupled extracellular miRNAs in mammalian follicular fluid and their potential involvement in cellto-cell communication are briefly discussed.

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The let-7g microRNA promotes follicular granulosa cell apoptosis by targeting transforming growth factor-β type 1 receptor

Cited by 59 publications

References 56 publications

A miRNA target network putatively involved in follicular atresia

A miRNA target network putatively involved in follicular atresia

MicroRNAs: New Insight in Modulating Follicular Atresia: A Review

Dynamics and role of MicroRNAs during mammalian follicular development

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