The Level of Glutamic Acid in the Semen of Male White Rat (Ratus Norwegicus) after Being Treated with Tannin of Pluchea Indica

Abstract

This current research aims at proving that the treatment of Pluchea indica's tannin could affect the level of glutamic acid in the semen of male white rat. Experimental method was employed, under the following details: control group (without being treated) and experiment group with the treatment of Pluchea indica's tannin. The observation was conducted based upon the treatment; the white rats were dissected, and vas deferens is taken to get the semen of the white rat. The amino acid of the semen was then analyzed by utilizing high-performance liquid chromatography. The data are analyzed by using ANOVA. The results show that there is an effect after the treatment of Pluchea indica's tannin on the level of glutamic acid in the semen of male white rat (p < 0.05). The male white rats in the control group had averagely 1 547.48 mg · (100 g) -1 glutamic acid level; while the male white rats in the experiment group (after the treatment of Pluchea indica's tannin) showed the decrease on glutamic acid level (1 494.43 mg · (100 g) -1 to 1 341.40 mg · (100 g) -1 ). The high and low level of glutamic acid determined the quality of spermatozoa.

Phytoconstituents and Pharmacological Activities of Indian Camphorweed (Pluchea indica): A Multi-Potential Medicinal Plant of Nutritional and Ethnomedicinal Importance

“…The base is blunted, and leaf bones are pinnate-formed with parchment-like intervenium. An aroma is produced when the leaf blades are crushed [20][21][22]. The stem is strong, rigid, woody, and round with monopodial branches.…”

“…In Yogyakarta, Indonesia, the leaves are used as a galactagogue to maintain, induce, and augment maternal milk production [40]. It is used orally as anti-fertility for men [20,21]. In Peninsular Malaysia, it is cultivated in gardens for its young shoots, which can be eaten raw.…”

“…Many plants and their constituents have been investigated for their antifertility potential [105]. Interestingly, P. indica has been used orally in men as an antifertility alternative medicine [20,21,106].…”

“…It was reported that the leaf extract (doses 1.4, 2.8, and 5.6 mg/kg for 10 days) caused a decrease in the number of spermatid cells in male mice when the dose increased [106]. It was found that the treatment of white male adult rats with leaf tannin reduced the number of spermatozoa and spermatids [20,21]. Further, tannin remarkably reduced glutamic acid levels with an average decrease ranging from 494.43 to 341.40 mg/100 g in the semen of male white rats in comparison to the control group (547.48 mg/100 g glutamic acid level) [21,108].…”

“…It was found that the treatment of white male adult rats with leaf tannin reduced the number of spermatozoa and spermatids [20,21]. Further, tannin remarkably reduced glutamic acid levels with an average decrease ranging from 494.43 to 341.40 mg/100 g in the semen of male white rats in comparison to the control group (547.48 mg/100 g glutamic acid level) [21,108]. Glutamic acid is needed for spermatozoa metabolism, and decreasing its level leads to the reduction in spermatozoa motility [109].…”