The lignin synthesis related genes and lodging resistance of Fagopyrum esculentum

Hu, Dong; Liu, X. B.; She, Hengzhi; Gao, Zhiqiang; Ruan, Renwu; Wu, Di; Yi, Zelin

doi:10.1007/s10535-016-0685-4

Cited by 50 publications

(33 citation statements)

References 43 publications

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“…The results of correlation analysis showed that lodging resistance index was significantly positively correlated with breaking strength, which was confirmed by calculating lodging resistance index. Previous studies showed that severe shading can affect some enzymes related to lignin biosynthesis, such as 4CL, CAD and PAL, as well as the expression level of their biosynthetic genes, so as to reduce the biosynthesis of lignin and affect lodging resistance [5,37]. Correlation analysis also showed that the key enzymes of lignin biosynthesis (PAL, POD, 4CL) were significantly correlated with lignin content, xylem proportion and lodging resistance.…”

Section: Discussionmentioning

confidence: 92%

Slight Shading Stress at Seedling Stage Does not Reduce Lignin Biosynthesis or Affect Lodging Resistance of Soybean Stems

et al. 2020

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Shade is widespread in agricultural production and affects lignin biosynthesis and lodging resistance of crops. We explored the effects of shade intensity on lignin biosynthesis and lodging resistance at the physiological and molecular levels in two soybean cultivars (Nandou12 and E93) with different shade tolerance under four progressively severe shade treatments, S0-S3 (S0: no shade, S1: slight shade, S2: moderate shade, S3: heavy shade). Our results showed no significant difference in breaking strength of the two cultivars under S1 and S0 treatments, with no prominent decrease in the lodging resistance index. The activity of lignin biosynthesis rate-limiting enzymes phenylalanine ammonia-lyase (PAL), peroxidase and cinnamyl alcohol dehydrogenase (CAD), which were considerably related to the two lodging resistance indexes above, was not significantly decreased by slight shade, while 4-coumaric acid ligase (4CL) activity was increased. Most genes involved in lignin biosynthesis were not significantly down-regulated by slight shade (S1) compared to S0, while p-coumarate 3-hydroxylase (C3H), 4-coumaric acid ligase (4CL) and laccase (LAC) genes were upregulated. Under heavy shade (S3), enzyme activity and gene expression associated with lignin synthesis in both soybean cultivars were strongly inhibited; moreover, stem mechanical strength and lodging resistance were remarkably decreased compared with those under S0. These physiological and molecular changes suggested that applicable shade levels do not significantly affect the mechanical strength and lodging resistance of soybean stem. Exploiting the lodging resistance potential of existing soybean cultivars was an effective and efficient way to address yield reduction caused by lodging in intercropped soybeans.

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Section: Discussionmentioning

confidence: 92%

Slight Shading Stress at Seedling Stage Does not Reduce Lignin Biosynthesis or Affect Lodging Resistance of Soybean Stems

et al. 2020

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“…The deposition of cell-wall materials, particularly lignin, is mainly regulated by defense-related enzymes (such as PAL, LOX, POX, PPO, chitinase, etc.) for lodging resistance in plants against invading pathogens [59]. PAL and POX enzymes play a requisite role in strengthening plant defense via structural deposition of lignin and phenolic compounds and also act as antagonistic to the invading pathogens by the production of phytoalexins (PAL), thereby hampering the growth and development of invading pathogen [55,59].…”

Section: Discussionmentioning

confidence: 99%

“…for lodging resistance in plants against invading pathogens [59]. PAL and POX enzymes play a requisite role in strengthening plant defense via structural deposition of lignin and phenolic compounds and also act as antagonistic to the invading pathogens by the production of phytoalexins (PAL), thereby hampering the growth and development of invading pathogen [55,59]. Similarly, the PGPF-treated chilli seedlings subjected to challenge inoculation showed the enhanced activity of PAL and POX enzymes compared to untreated challenge-inoculated seedlings, thereby authenticating the results obtained under histochemical studies.…”

Section: Discussionmentioning

confidence: 99%

Plant Growth-Promoting Fungi (PGPF) Instigate Plant Growth and Induce Disease Resistance in Capsicum annuum L. upon Infection with Colletotrichum capsici (Syd.) Butler & Bisby

2019

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In the current study, a total of 70 fungi were isolated from the rhizosphere soil of chilli collected from six different districts of south Karnataka, India. All the rhizospheric fungi were evaluated for its antagonistic nature against Colletotrichum capsici—the causal agent of anthracnose disease—and eight isolates were found positive. The antagonistic fungi were further characterized for the production of plant growth-promoting traits wherein five isolates were recorded positive for all the traits tested and were also positive for root colonization. All five plant growth-promoting fungi (PGPF) were subjected to molecular characterization for identification up to the species level and the accession numbers were obtained from NCBI. The five isolates, namely NBP-08, NBP-45, NBP-61, NBP-66 and NBP-67, were further experimented with on susceptible seeds to evaluate its efficacy on seed and plant growth parameters along with induction of resistance against the anthracnose disease. The evaluated PGPF offered significant enhancement in seed and plant growth parameters with maximum improvement observed in seeds treated with NBP-61. Among the PGPF isolates, NBP-61 showed the maximum protection of 78.75%, while all the other isolates also showed significant protection against anthracnose disease compared to untreated plants. The higher accumulation of lignin and callose deposition along with enhanced defense enzyme activities in the PGPF-treated challenge-inoculated seedlings authenticated the protection offered by PGPF. The study evidenced the immense ability of PGPF in eliciting disease protection and enhancement of plant growth in chilli, which may act as a possible substitute for harmful chemicals.

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“…Numerous enzymes are involved in the synthesis of lignin, including phenylalanine ammonia-lyase (PAL), 4-coumaric acid coenzyme A ligase (4CL), cinnamyl alcohol dehydrogenase (CAD), cinnamate-4-hydroxylase (C4H), cinnamoyl-CoA reductase (CCR), and peroxidase (POD) (Hu et al, 2017;Mertz-Henning et al, 2015;Shi et al, 2019). Most of the enzymes in the lignin metabolic pathway are induced by light at the transcriptional level, such as PAL, the activity of which is closely related to C4H, 4CL, CAD, and CCR (Ferrer et al, 2008;Hemm et al, 2004).…”

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confidence: 99%

Response of Fruit Bagging to Lignin Biosynthesis and Expression of Related Genes in Fruit Peel of Sand Pear (Pyrus pyrifolia Nakai) cv. Cuiguan

Shi¹,

Wang²,

Zhang³

et al. 2019

horts

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This study explored the effects of different colored bags (blue, green, white, yellow, orange, and red) on russet deposition on the peel of semi-russet ‘Cuiguan’ pears 10 days after full bloom (DAFB). The process of russeting of the peel and structure of the cork layer were characterized by microscopy and scanning electron microscopy (SEM), followed by the detection of lignin and the activity of enzymes involved in lignin synthesis. The expression of cinnamate-4-hydroxylase, 4-coumarate:coenzyme A ligase, cinnamyl alcohol dehydrogenase, cinnamoyl-CoA reductase, and peroxidase, which were related to phenylalanine ammonia-lyase, was determined via real-time quantitative polymerase chain reaction. Russeting of the outer peel of ‘Cuiguan’ pear accumulated rapidly at 80 DAFB, and a positive relationship between the russet index and lignin content was observed. Red and infrared (IR) ray, partial far-IR light (600–800 nm), and ultraviolet-A light (350–400 nm) promoted russeting in ‘Cuiguan’ pear peel, whereas green light decreased russeting, the russet index, enzymatic activities, and the expression levels of enzymes involved in lignin synthesis. Values of all these factors were higher for ‘Cuiguan’ pears in red bags than for those in bags of other colors. These findings suggested that spectral components affected the synthesis of lignin and the formation of fruit russet. Storage in green bags reduced russeting and improved fruit appearance.

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The lignin synthesis related genes and lodging resistance of Fagopyrum esculentum

Cited by 50 publications

References 43 publications

Slight Shading Stress at Seedling Stage Does not Reduce Lignin Biosynthesis or Affect Lodging Resistance of Soybean Stems

Slight Shading Stress at Seedling Stage Does not Reduce Lignin Biosynthesis or Affect Lodging Resistance of Soybean Stems

Plant Growth-Promoting Fungi (PGPF) Instigate Plant Growth and Induce Disease Resistance in Capsicum annuum L. upon Infection with Colletotrichum capsici (Syd.) Butler & Bisby

Response of Fruit Bagging to Lignin Biosynthesis and Expression of Related Genes in Fruit Peel of Sand Pear (Pyrus pyrifolia Nakai) cv. Cuiguan

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