The LINC00452/miR-204/CHST4 Axis Regulating Thymic Tregs Might Be Involved in the Progression of Thymoma-Associated Myasthenia Gravis

Abstract

BackgroundMyasthenia gravis (MG) is an autoimmune disease that mainly affects neuromuscular junctions and is usually associated with immune disorders in the thymoma. The competitive endogenous RNA (ceRNA) hypothesis has been demonstrated to be an intrinsic mechanism regulating the development of several autoimmune diseases; however, the mechanism where the ceRNA network regulates immune cells in patients with thymoma-associated MG (TAMG) has rarely been explored.MethodsRNA-seq data and clinical information of 124 patients with thymoma were obtained from The Cancer Genome Atlas (TCGA) database. The patients were divided into two groups according to whether they were diagnosed with MG. We applied the propensity score matching method to reduce the incidence of baseline confounders. We then constructed a ceRNA network with differentially expressed RNAs between the groups based on four public databases. The expression of genes of interest was validated by qPCR. Moreover, we predicted the immune cells that infiltrated the thymoma and then analyzed the association between immune cells and RNA in the ceRNA network. To further determine the function of the mRNAs associated with immune cells in patients with TAMG, we performed gene set enrichment analysis in thymoma patients with MG.ResultsAfter matching, 94 patients were included in the following analysis. A total of 847 mRNAs, 409 lncRNAs, and 45 miRNAs were differentially expressed between the groups. The ceRNA network, including 18 lncRNAs, four miRNAs, and 13 mRNAs, was then constructed. We then confirmed that CHST4 and LINC00452, miR-204-3p and miR-204-5p were differentially expressed between patients with TAMG and thymoma patients without MG (NMG) by qPCR. Moreover, we found that the percentage of predicted regulatory T (Treg) cells was significantly decreased in patients with TAMG. Further analysis indicated that the LINC00452/miR-204/CHST4 axis might regulate thymic regulatory T cells (Tregs) in the progression of MG.ConclusionsIn this research, we constructed a ceRNA network involved in the progression of TAMG, discovered that thymic Tregs were significantly decreased in patients with TAMG, and assumed that the LINC00452/miR-204/CHST4 axis may regulate thymic Tregs in the development of TAMG. These findings may deepen our understanding of the roles of the ceRNA network in regulating TAMG and highlight the function of CHST4 in recruiting peripheral T cells in the progression of TAMG.

CHST4 Gene as a Potential Predictor of Clinical Outcome in Malignant Pleural Mesothelioma

“…CHST4 is a sulfotransferase expressed in HEVs [8] and has an important role in the immune system, given that it is involved in homing of lymphocytes in lymphoid organs [25]. It is assumed that CHST4 is involved in tumor immunity against malignant tumors, and functional analysis of CHST4 is expected to contribute to the development of immunotherapy.…”

Epigenetics of Thymic Epithelial Tumors

“…Interestingly, the immune-related lncRNAs AP000787.1, AC004943.1, WT1-AS, and FOXG1-AS1 expression are regulated by the methylation of the promoter regions [150]. Moreover, the competing endogenous RNA (ceRNA) network LINC00452/miR-204/CHST4 was recently proposed as a crucial axis, which regulates thymic regulatory T cells (Tregs) and the development of TAMG [151].…”

SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells

“…The primers originated from GenePharma. 2 -△△CT was applied for data quanti cation as previously described [19]. The primers originated from GenePharma.…”