The Links Between Insect Symbionts and Insecticide Resistance: Causal Relationships and Physiological Tradeoffs

Pietri, Jose E.; Liang, Dangsheng

doi:10.1093/aesa/say009

Cited by 55 publications

(38 citation statements)

References 54 publications

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“…Bacterial insolates have also been found to in uence mateselection behavior in B. dorsalis [46]. Bactrocera dorsalis also bene ts from the ability of some of its gut associated bacteria to break down toxicants, which has been linked to insecticide resistance in this species [43,47].…”

Section: Introductionmentioning

confidence: 99%

Influence of inoculated gut bacteria on the development of Bactrocera dorsalis and on its susceptibility to the entomopathogenic fungus, Metarhizium anisopliae

Gichuhi

Khamis

Berg

et al. 2020

Preprint

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Background: Symbiotic interactions between insects and bacteria have been associated with a vast variety of physiological, ecological and evolutionary consequences for the host. A wide range of bacterial communities have been found in association with the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), an important pest of cultivated fruit in most regions of the world. We evaluated the diversity of gut bacteria in B. dorsalis specimens from several populations in Kenya and investigated the roles of individual bacterial isolates in the development of axenic (germ-free) B. dorsalis fly lines and their responses to the entomopathogenic fungus, Metarhizium anisopliae. Results: We sequenced 16S rRNA to evaluate microbiomes and coupled this with bacterial culturing. Bacterial isolates were mono-associated with axenic B. dorsalis embryos. The shortest embryonic development period was recorded in flies with an intact gut microbiome while the longest period was recorded in axenic fly lines. Similarly, larval development was shortest in flies with an intact gut microbiome, in addition to flies inoculated with Providencia alcalifaciens. Adult B. dorsalis flies emerging from embryos that had been mono-associated with a strain of Lactococcus lactis had decreased survival when challenged with a standard dosage of M. anisopliae ICIPE69 conidia. However, there were no differences in survival between the germ-free lines and flies with an intact microbiome. Conclusions: These findings will contribute to the selection of probiotics used in artificial diets for B. dorsalis rearing and the development of improved integrated pest management strategies based on entomopathogenic fungi.

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Section: Introductionmentioning

confidence: 99%

Influence of inoculated gut bacteria on the development of Bactrocera dorsalis and on its susceptibility to the entomopathogenic fungus, Metarhizium anisopliae

Gichuhi

Khamis

Berg

et al. 2020

Preprint

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“…For instance, the presence of certain early colonizers during development, such as Snodgrassella alvi, can modulate phase I detoxification pathways by affecting the expression of cytochrome P450 (CYP) enzymes (Schwarz et al, 2016) that are critical for pesticide degradation (Claudianos et al, 2006). We do not yet know how gut colonizers influence detoxification of pesticides in honey bees under natural conditions, though findings in other insects suggest a major role for symbiont-mediated regulation of detoxification pathways (Pietri and Liang, 2018). As the gut microbiota is dependant on caste (Jones et al, 2018), diet (Maes et al, 2016;Zheng et al, 2017), and environmental factors (Jones et al, 2017), including exposure to some pesticides (Dai et al, 2018), studies should test the ability of the microbiota to either directly degrade pesticides or promote indirect detoxification of them.…”

Section: Pesticides Affect Detoxification and Metabolism In Honey Beesmentioning

confidence: 99%

Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress

et al. 2020

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Managed populations of the European honey bee (Apis mellifera) support the production of a global food supply. This important role in modern agriculture has rendered honey bees vulnerable to the noxious effects of anthropogenic stressors such as pesticides. Although the deleterious outcomes of lethal pesticide exposure on honey bee health and performance are apparent, the ominous role of sublethal pesticide exposure is an emerging concern as well. Here, we use a data harvesting approach to better understand the toxicological effects of pesticide exposure across the honey bee life cycle. Through compiling adult-and larval-specific median lethal dose (LD 50) values from 93 published data sources, LD 50 estimates for insecticides, herbicides, acaricides, and fungicides are highly variable across studies, especially for herbicides and fungicides, which are underrepresented in the meta-data set. Alongside major discrepancies in these reported values, further examination of the compiled data suggested that LD 50 may not be an ideal metric for honey bee risk assessment. We also discuss how sublethal effects of pesticide exposure, which are not typically measured in LD 50 studies, can diminish honey bee reproduction, immunity, cognition, and overall physiological functioning, leading to suboptimal honey bee performance and population reduction. In consideration of actionable solutions to mitigate the effects of sublethal pesticide exposure, we have identified the potential for probiotic supplementation as a promising strategy that can be easily incorporated alongside current agricultural infrastructure and apicultural management practices. Probiotic supplementation is regularly employed in apiculture but the potential for evidence-based targeted approaches has not yet been fully explored within a formal toxicological context. We discuss the benefits, practical considerations, and limitations for the use and delivery of probiotics to hives. Ultimately, by subverting the sublethal effects of pesticides we can help improve the long-term survival of these critical pollinators.

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“…Bacterial insolates have also been found to in uence mate-selection behavior in B. dorsalis [31]. Bactrocera dorsalis also bene ts from the ability of some of its gut associated bacteria to break down toxicants, which has been linked to insecticide resistance in this species [30,32].…”

Section: Introductionmentioning

confidence: 99%

Influence of Bactrocera Dorsalis Larval Gut Bacteria on its Susceptibility to the Entomopathogenic Fungus, Metarhizium Anisopliae

Gichuhi¹,

Khamis²,

Berg³

et al. 2020

Preprint

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Symbiotic interactions between insects and bacteria have been associated with a vast variety of physiological, ecological and evolutionary consequences for the host. A wide range of bacterial communities have been found in association with the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), an important pest of cultivated fruit in most regions of the world. We evaluated the diversity of gut bacteria in B. dorsalis specimens from several populations in Kenya and investigated the roles of individual bacterial isolates in the development of axenic B. dorsalis fly lines and their responses to the entomopathogenic fungus, Metarhizium anisopliae. We utilized a high throughput sequencing approach of the bacterial 16S rRNA gene to evaluate microbiomes, coupled with culture and mono-association for the assay. The shortest embryonic development period was recorded in flies with an intact gut microbiome while the longest period was recorded in germ-free fly lines. Similarly, larval development was shortest in flies with an intact gut microbiome. Adult B. dorsalis flies emerging from embryos inoculated with a strain of Lactococcus lactis had decreased survival when challenged with a standard dosage of M. anisopliae ICIPE69 conidia. However, there were no differences in survival between the germ-free lines and flies with an intact microbiome. These findings contribute to the selection of probiotics used in artificial diets for B. dorsalis rearing and the development of improved integrated pest management strategies based on entomopathogenic fungi.

show abstract

The Links Between Insect Symbionts and Insecticide Resistance: Causal Relationships and Physiological Tradeoffs

Cited by 55 publications

References 54 publications

Influence of inoculated gut bacteria on the development of Bactrocera dorsalis and on its susceptibility to the entomopathogenic fungus, Metarhizium anisopliae

Influence of inoculated gut bacteria on the development of Bactrocera dorsalis and on its susceptibility to the entomopathogenic fungus, Metarhizium anisopliae

Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress

Influence of Bactrocera Dorsalis Larval Gut Bacteria on its Susceptibility to the Entomopathogenic Fungus, Metarhizium Anisopliae

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