The lipid metabolism of blue-green algae

Nichols, B.W.; Harris, R. V.; James, A. T.

doi:10.1016/0006-291x(65)90356-6

Cited by 142 publications

(43 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…51900) according to Juaneda and Rocquelin (1985). Total and individual phospholipids were determined in aliquots of the lipid extract, separated by TLC (Merck, No 1.05721, Silica gel 60), developed in chloroform:methanol:acetic acid: water (85:15:10:3.5, v/v), (Nichols et al 1965), as described by Pukacki and Kamiñska-Ro¿ek (2002). The bands were scraped off for phosphorus analysis.…”

Section: Membrane Compositionmentioning

confidence: 99%

Environmental pollution changes in membrane lipids, antioxidants and vitality of Scots pine (Pinus sylvestris L.) pollen

Pukacki

Chalupka

2011

Acta Soc Bot Pol

View full text Add to dashboard Cite

Investigations were carried out on pollen grains of Scots pine (Pinus sylvestris L.) collected from trees at 1.5, 3, 4 km and control, 20 km from the Luboñ factory producing mineral fertilisers. The percentage of germination of pollen formed close to the pollution source was ca 20% lower compared to the control pollen. Lowered vitality of the pollen was effected in changes of the structure of cytoplasmic membranes. Pollen from the polluted area contained ca 15% less total phospholipids, mainly phosphatidylcholine and phosphatytidylinositol and had a lower content of soluble proteins and less of low molecular antioxidants, such as thiols and ascorbic acid. Composition of total fatty acid in phospholipids fractions showed a significant reduction in the degree of unsaturation of fatty acids. Pollen originating from the polluted area and stored at -30°C showed considerably stronger degradation of cytoplasmic membranes than control.

show abstract

Section: Membrane Compositionmentioning

confidence: 99%

Environmental pollution changes in membrane lipids, antioxidants and vitality of Scots pine (Pinus sylvestris L.) pollen

Pukacki

Chalupka

2011

Acta Soc Bot Pol

View full text Add to dashboard Cite

show abstract

“…methanol\acetic acid\water (85 : 15 : 10 : 3.5, by vol.) [6]. Radioactive zones were located by autoradiography.…”

Section: Methodsmentioning

confidence: 99%

Re-evaluation of plant sulpholipid labelling from UDP-[14C]glucose in pea chloroplasts

Roy

Harwood

1999

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…The samples (5 g each) of needles were ground to powder in liquid nitrogen, and the lipids were extracted with chloroform:methanol (2:1, v:v), according to Juaneda and Requelin [27] and Nichols et al [28]. The separation of polar and neutral lipids was performed using a SEP-PAK Silica cartridge (Waters As, No.…”

Section: Separation and Identification Of Phospholipidsmentioning

confidence: 99%

Differential effects of spring reacclimation and deacclimation on cell membranes of Norway spruce seedlings

Pukacki

Kamińska-Rożek

2013

Acta Soc Bot Pol

View full text Add to dashboard Cite

Two-year-old seedlings of Norway spruce (Picea abies) during spring deacclimation were subjected to controlled reacclimation by exposure to low temperatures of 4/−3°C (day/night) in a cold room. The highest increase in freezing tolerance (by 7°C) was observed after 12 d of low temperature exposure, when shoot water potential (Ψ_{w shoot}) decreased to 0.64 MPa. The process of reacclimation was accompanied by an increase in the phospholipid content of needle cell membranes. This increase applied to total (PL) and individual phospholipids: phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidic acid (PA). After being exposed to the low temperature for 18 d, the seedlings were moved into the open air. This caused deacclimation, with an increase in Ψ_{w shoot} to −0.36 MPa and a decrease in the total phospholipid content and freezing tolerance of the needles. Significant correlations were observed between freezing tolerance, the membrane permeability (MP) of the needles and the phospholipid content, Ψ_{w shoot} and water content of the needles. The results show that during spring deacclimation, Norway spruce seedlings can be subjected to reacclimation, which is reflected in the phospholipid content, the biophysical changes of the membranes, and the freezing tolerance of the seedlings. During both spring deacclimation and reacclimation, water content in the needles plays a critical role in the cold tolerance of spruce seedlings

show abstract

The lipid metabolism of blue-green algae

Cited by 142 publications

References 8 publications

Environmental pollution changes in membrane lipids, antioxidants and vitality of Scots pine (Pinus sylvestris L.) pollen

Environmental pollution changes in membrane lipids, antioxidants and vitality of Scots pine (Pinus sylvestris L.) pollen

Re-evaluation of plant sulpholipid labelling from UDP-[14C]glucose in pea chloroplasts

Differential effects of spring reacclimation and deacclimation on cell membranes of Norway spruce seedlings

Contact Info

Product

Resources

About