The lipoxygenase pathway in zebrafish. Expression and characterization of zebrafish ALOX5 and comparison with its human ortholog

Adel, Susan; Heydeck, Dagmar; Kühn, Hartmut; Ufer, Christoph

doi:10.1016/j.bbalip.2015.10.001

Cited by 23 publications

(18 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increasing concentrations of PGE2 inhibit 5-LO translocation from the cytoplasm to the nucleus, abrogating leukotriene synthesis (48), and skewing the balance between pro-and anti-inflammatory mediators towards increased lipoxin synthesis -a process termed "lipid-mediator class switching" (28). Zebrafish have the three key lipoxygenase genes for lipid mediator synthesis: alox12, alox5 and alox15b genes (49)(50)(51). To assess the contribution of lipoxin production during inflammation resolution in the zebrafish, we immersed injured transgenic larvae in PD146176, a specific 15-LO inhibitor (52).…”

Section: Upregulation Of Lipoxygenase By Pge2 Is Necessary For Inflammentioning

confidence: 99%

PGE₂production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo

Lee

Robertson

Steel

et al. 2017

Preprint

View full text Add to dashboard Cite

Neutrophils are the first immune cells recruited to a site of injury or infection, where they perform many functions. Having completed their role, neutrophils must be removed from the inflammatory site -either by apoptosis and efferocytosis or by reverse migration away from the wound -for restoration of normal tissue homeostasis. Disruption of these tightly controlled physiological processes of neutrophil removal can lead to a range of inflammatory diseases. We used an in vivo zebrafish model to understand the role of lipid mediator production in neutrophil removal. Following tailfin amputation in the absence of macrophages, neutrophillic inflammation does not resolve. This is due to loss of macrophage-dependent production of eicosanoid prostaglandin E2, which drives neutrophil removal via promotion of reverse migration.

show abstract

Section: Upregulation Of Lipoxygenase By Pge2 Is Necessary For Inflammentioning

confidence: 99%

PGE₂production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo

Lee

Robertson

Steel

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

“…Growth systems that can offer controlled cultivation conditions can be used as an easy tool for the production of a variety of proteins in E. coli —usually E. coli BL21 (DE3). For example, the multifunctional enzyme 2 from Drosophila [ 27 ] and the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis [ 34 ] enabling crystallization of both proteins, Sdr proteins from Stapholococcus areus [ 35 ], a zinc-dependent metalloprotease Zmp1 from Clostridium difficile [ 36 ], human proteins such as different arachidonate lipoxygenase enzymes [ 37 , 38 ], lipoxygenase from Pseudomonas aeruginosa [ 39 ] and zebrafish [ 40 ], the cytosolic response regulator LiaR from Enterococcus faecalis expressed in the pETDuet vector [ 41 ], the metal-dependent surface lipoprotein SitA from Staphylococcus pseudointermedius [ 42 ] or another metalloenzyme like the alkane monooxygenase AlkB [ 43 ] were successfully produced employing the EnBase technology. Furthermore, a family VIII esterase Est22 [ 44 ], the E. coli chloramphenicol acetyltransferase I (CATI) [ 45 ], different sortase enzymes from Streptococcus [ 46 ], and proteins which do not express in commonly applied media like the phosphoprotein nucleolin [ 47 ] and eukaryotic ribonuclease inhibitor [ 22 , 48 ] could be successfully expressed in E. coli by the application of an advanced growth system.…”

Section: Introductionmentioning

confidence: 99%

The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli

Krause

Neubauer²,

Neubauer

2016

Microb Cell Fact

View full text Add to dashboard Cite

While the nutrient limited fed-batch technology is the standard of the cultivation of microorganisms and production of heterologous proteins in industry, despite its advantages in view of metabolic control and high cell density growth, shaken batch cultures are still the standard for protein production and expression screening in molecular biology and biochemistry laboratories. This is due to the difficulty and expenses to apply a controlled continuous glucose feed to shaken cultures. New ready-made growth media, e.g. by biocatalytic release of glucose from a polymer, offer a simple solution for the application of the fed-batch principle in shaken plate and flask cultures. Their wider use has shown that the controlled diet not only provides a solution to obtain significantly higher cell yields, but also in many cases folding of the target protein is improved by the applied lower growth rates; i.e. final volumetric yields for the active protein can be a multiple of what is obtained in complex medium cultures. The combination of the conventional optimization approaches with new and easy applicable growth systems has revolutionized recombinant protein production in Escherichia coli in view of product yield, culture robustness as well as significantly increased cell densities. This technical development establishes the basis for successful miniaturization and parallelization which is now an important tool for synthetic biology and protein engineering approaches. This review provides an overview of the recent developments, results and applications of advanced growth systems which use a controlled glucose release as substrate supply.

show abstract

“…Until now, our knowledge about the detailed biological functions of LOXs isoforms is limited [37], and known arachidonate LOXs contain arachidonate 5-lipoxygenase (5-LOX, ALOX5, 15-LO-1, 15-LOX-1), 12-LOX, arachidonate 12-lipoxygenase type II (ALOX12B, 12R-LOX), arachidonate 15-lipoxygenase (ALOX15), and ALOX15 type II (ALOX15B) [200].…”

Section: Various Lox Enzymesmentioning

confidence: 99%

“…Increased expression of Cox-2 and ALOX5 are reported in lung cancer and knocking-out 5-LOX resulted into progression [216]. In this regard 5-LOX blockade resulted in an increase of apoptosis [200]. 5-LOX was shown to be higher expressed in HCC versus normal liver tissues and inhibiting 5-LOX induces apoptosis and blocks cancer progression [217].…”

Section: Various Lox Enzymesmentioning

confidence: 99%

Eicosanoids in carcinogenesis

Brücher

Jamall

2019

4open

View full text Add to dashboard Cite

Inflammation is the body's reaction to pathogenic (biological or chemical) stimuli and covers a burgeoning list of compounds and pathways that act in concert to maintain the health of the organism. Eicosanoids and related fatty acid derivatives can be formed from arachidonic acid and other polyenoic fatty acids via the cyclooxygenase and lipoxygenase pathways generating a variety of pro- and anti-inflammatory mediators, such as prostaglandins, leukotrienes, lipoxins, resolvins and others. The cytochrome P450 pathway leads to the formation of hydroxy fatty acids, such as 20-hydroxyeicosatetraenoic acid, and epoxy eicosanoids. Free radical reactions induced by reactive oxygen and/or nitrogen free radical species lead to oxygenated lipids such as isoprostanes or isolevuglandins which also exhibit pro-inflammatory activities. Eicosanoids and their metabolites play fundamental endocrine, autocrine and paracrine roles in both physiological and pathological signaling in various diseases. These molecules induce various unsaturated fatty acid dependent signaling pathways that influence crosstalk, alter cell–cell interactions, and result in a wide spectrum of cellular dysfunctions including those of the tissue microenvironment. Although the complete role of eicosanoids, including that of the recently elucidated anti-inflammatory specialized pro-resolving lipid mediators (SPMs), e.g. lipoxins, resolvins, protectins and maresins, is not completely understood, the result of unremitting chronic inflammation is fostering early stages of carcinogenesis. Chronic inflammation facilitates the transition from a normal cell to a cancerous one. The disruption of homeostasis across a wide, but identifiable, swath of diverse molecular pathways creates a micromilieu which constitutes an early and necessary step in the 6-step sequence of carcinogenesis for the vast majority of cancers, termed “sporadic cancers”.

show abstract

The lipoxygenase pathway in zebrafish. Expression and characterization of zebrafish ALOX5 and comparison with its human ortholog

Cited by 23 publications

References 57 publications

PGE₂production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo

PGE₂production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo

The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli

Eicosanoids in carcinogenesis

Contact Info

Product

Resources

About

The lipoxygenase pathway in zebrafish. Expression and characterization of zebrafish ALOX5 and comparison with its human ortholog

Cited by 23 publications

References 57 publications

PGE2production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo

PGE2production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo

The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli

Eicosanoids in carcinogenesis

Contact Info

Product

Resources

About

PGE₂production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo

PGE₂production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo