The Liquid Chromatography of Some Benzodiazepines

The application of HPLC to the determination of some 1,4 benzodiazepines and their metabolites in plasma.

“…Dixon, Brooks, Postma, Hackman, Spector, Moore & Schwartz (1976) have reported that Ndesmethyldiazepam is a metabolite of chlordiazepoxide which is found in the plasma of patients receiving either single or chronic doses of chlordiazepoxide. Scott & Bommer (1970), using a Durapak OPN column were able to resolve N-desmethyldiazepam from diazepam, but we could not reproduce these results satisfactorily. The silica column (3) gave good resolution of N-desmethyldiazepam from diazepam, chlordiazepoxide and its metabolites ( Figure 5).…”

“…Scott & Bommer (1970) with a Durapak OPN column separated pure samples of diazepam, oxazepam and ten other benzodiazepines. HPLC systems have been described for the determination of partition coefficients of some 1,4-benzodiazepines (Hulshoff & Perrin, 1976).…”

Determination of benzodiazepine anticonvulsants in plasma by high-performance liquid chromatography

“…Flow rate was 60 mL/h at a pressure of 6.67 MPa (967 psi). The internal standard stock solution was made by dissolving Extractions of drug-free plasma (A) and drug-free plasma spiked with diazepam (1), clonazepam (3), and nordiazepam (5) at concentrations of 172 ng/mL, 53 ng/mL, and 461 ng/mL, respectively. 4,5-Dihydrodiazepam (2) was added as in Procedure.…”

“…Scott and Bommer (5) described an HPLC method for analysis of some benzodiazepines and applied it to analysis of animal urine. The chromatography was limited by the state of the art at that time, but the authors showed the potential of liquid chromatography for separation, identification, and quantitation of benzodiazepines in metabolite studies.…”

High Pressure Liquid Chromatography of Benzodiazepines: Analysis of Ketazolam

“…The amount injected onto the column ranged from a few nanograms (1) to several hundred nanograms (2). The high pressure liquid chromatography of some benzodiazepines was also reported (3). However, the true potential of the technique was not demonstrated since the amounts injected were in the microgram range and the mobile phase used caused some deterioration of the stationary phase.…”