The liquid–glass transition in sugars: Relaxation dynamics in trehalose

Cummins, H. Z.; Zhang, Hepeng; Oh, Jiyoung; Seo, Ja Young; Kim, Hyung Kook; Hwang, Yoon‐Hwae; Yang, Yoon Mee; Yu, Yun Sik; Inn, Yongwoo

doi:10.1016/j.jnoncrysol.2006.02.182

Cited by 28 publications

(24 citation statements)

References 38 publications

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“…The temperature dependence of relaxation times below the glass transition may follow the Arrhenius relationship, but changes above the glass transition become highly temperature-dependent and deviate from Arrhenian behavior which is used to classify materials to fragile and strong glass formers (Angell, 1993) The composition and water content of carbohydrate glass-formers has been found to affect their sub-T g and a-relaxations (Chan et al, 1986;Roos, 1995;Noel et al, 1996Noel et al, , 2000Champion et al, 2000;Le Meste et al, 2002;Psurek et al, 2004;Cummins et al, 2006;Kaminski et al, 2008a,b;Silalai and Roos, 2010b), but there is very little information on the miscibility and effects of composition on the relaxation behavior of mixtures of lowmolecular-weight carbohydrates and polymers. The objective of the present study was to determine effects of composition of milk solids at varying protein contents on their dielectric and mechanical a-relaxations as well as the temperature dependence of relaxation times around and above the glass transition.…”

Section: Introductionmentioning

confidence: 99%

Coupling of dielectric and mechanical relaxations with glass transition and stickiness of milk solids

Silalai

Roos

2011

Journal of Food Engineering

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Section: Introductionmentioning

confidence: 99%

Coupling of dielectric and mechanical relaxations with glass transition and stickiness of milk solids

Silalai

Roos

2011

Journal of Food Engineering

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“…Glass transition, or vitrification, is the process in which fluid practically solidifies without forming crystals. The glass transition temperature (Tg) of trehalose, for example, is high (115ºC) and as such it provides stability at relatively high supra-zero temperatures [52][53][54]. Trehalose is also unique in the fact that it does not completely lose its glass state even if it was slightly rehydrated.…”

Section: The Drying Industrymentioning

confidence: 99%

Exploring dry storage as an alternative biobanking strategy inspired by Nature

Saragusty

Loi

2019

Theriogenology

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Close to 25% of species in the animal kingdom face the risk of extinction. A variety of stresses, largely anthropogenic in nature, and lack of sufficient resources and political support to counteract this biodiversity crisis, suggest that we will witness the extinction of many thousands of species in the coming years. Unless we act now to, at least, preserve samples from each and every threatened species, these extinct species will be lost forever. To preserve record of the world's biodiversity, establishment of genome resource banks is thus desirable. Presently, biobanking is done almost exclusively by cryopreservation, followed by maintenance of the samples under liquid nitrogen. Cryopreservation has satisfactory efficiency but it comes with a host of problems. The process is also highly species-specific. Like in many other walks of life, we can search Nature for better alternatives. When longterm preservation is desired, Nature opted for controlled drying rather than in water preservation via freezing. Nature also created an assortment of materials that protect organisms from damages during the drying and rehydration processes. Once dry, these organisms can survive extended periods of time and be resistant to extreme environmental stressors. Over the past 70 years researchers attempted applying this idea to preserved desiccation-sensitive mammalian cells in the dry form. Desiccation is experiencing increased interest in recent years with some exciting developments. Presented here an overview of dry preservation and its possible applications towards establishment of dry biobanks as part of our endeavour to preserve the world's biodiversity.

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“…Sugar vitrification is a well‐established mechanism in anhydrobiosis for both plants and animals, which is generally associated with membrane and protein stabilization . Trehalose vitrifies at low water contents, but the capacity for protection is impacted by the glass transition temperature (Tg), which dictates the temperature and degree of hydration where the sugar will form or maintain a glassy state . Although the mechanism by which IDPs can reinforce and/or stabilize sugar glasses is not well understood, some LEA proteins and peptides have been shown to increase the Tg of sugar glasses .…”

Section: Disorder: Regulatory Element or Essential Property For Hydramentioning

confidence: 99%

Role of Intrinsic Disorder in Animal Desiccation Tolerance

2018

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This review compares the molecular strategies employed by anhydrobiotic invertebrates to survive extreme water stress. Intrinsically disordered proteins (IDPs) play a central role in desiccation tolerance in all species investigated. Various hypotheses about the functions of anhydrobiosis-related intrinsically disordered (ARID) proteins, including late embryogenesis abundant (LEA) and tardigrade-specific intrinsically disordered proteins, are evaluated by broad sequence characterization. A surprisingly wide range in sequence characteristics, including hydropathy and the frequency and distribution of charges, is discovered. Interestingly, two clusters of similar proteins are found that potentially correlate with distinct functions. This may indicate two broad groups of ARID proteins, composed of one group that folds into functional conformations during desiccation and a second group that potentially displays functions in the hydrated state. A broad range of physiochemical properties suggest that folding may be induced by factors such as hydration level, molecular crowding, and interactions with binding partners. This plasticity may be required to fine-tune the ARID-proteome response at different hydration levels during desiccation. Furthermore, the sequence properties of some LEA proteins share qualities with IDPs known to undergo liquid-liquid phase separations during environmental challenges.

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The liquid–glass transition in sugars: Relaxation dynamics in trehalose

Cited by 28 publications

References 38 publications

Coupling of dielectric and mechanical relaxations with glass transition and stickiness of milk solids

Coupling of dielectric and mechanical relaxations with glass transition and stickiness of milk solids

Exploring dry storage as an alternative biobanking strategy inspired by Nature

Role of Intrinsic Disorder in Animal Desiccation Tolerance

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