The lmo0501 gene coding for a putative transcription activator protein in Listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions

Abstract

In L. monocytogenes EGDe, the lmo0501 gene locus encodes a protein similar to the mannitol transcription regulator (MltR) protein in B. subtilis and B. stearothermophilus. In this study we investigated its functional role in L. monocytogenes EGDe cells in view of growth under different stress conditions. Increased lmo0501 gene expression at mRNA level was detected in response to cold, osmotic and organic acid stress exposure. An EGDe Δlmo0501 mutant strain was diminished in growth compared to the wild type strain in minimal defined medium containing either glucose or fructose, as carbon sources. Furthermore the lmo0501 null mutant had retarded growth under cold (4°C), salt (NaCl) and organic acid stress conditions compared to the parental wild type strain. Our results confirm the role of the lmo0501 gene in view of adaptation of L. monocytogenes cells to stress conditions as well as a contribution to the efficient utilization of glucose and fructose as carbon sources.Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-60467 Originally published at: Michel, E. The lmo0501 gene coding for a putative transcription activator protein in Listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions. 2011, University of Zurich, Vetsuisse Faculty.

Different Transcriptional Responses from Slow and Fast Growth Rate Strains of Listeria monocytogenes Adapted to Low Temperature

“…All these capacities indicate the existence of different mechanisms involved in the acclimation and adaptation phases that are able to respond against different temperatures ( Bresolin et al, 2008 ), suggesting the presence in L. monocytogenes strains of plasticity genome events, plasmid elements or transcriptional regulation such as sigma factor protein ( Becker et al, 2000 ), CodY, proteins encoded by lmo0287 ( Chan et al, 2007 ) and lmo0501 ( Michel et al, 2011 ). These sensors convert the input signals into gene regulation, allowing the bacterium to survive and adapt its metabolism to a new environment and maintain its homeostasis ( Chan et al, 2007 ; Metselaar et al, 2015 ).…”

Construction of Listeria monocytogenes Mutants with In‐Frame Deletions in the Phosphotransferase Transport System (PTS) and Analysis of Their Growth under Stress Conditions

“…For example, mannose‐specific PTS was regulated by the σ 54 factor encoded by rpoN (Gravesen and others ; Dalet and others ; Arous and others ; Vadyvaloo and others ). The gene that is upstream of the LMOf2365_0442 has been shown to be responsible for growth under cold, osmotic, and acid stress conditions (Michel and others ).…”

A Listeria monocytogenes RNA Helicase Essential for Growth and Ribosomal Maturation at Low Temperatures Uses Its C Terminus for Appropriate Interaction with the Ribosome

“…Several factors have been identified that allow Listeria to grow at low temperatures (12). Among these are compatible solute transporters encoded by the opu and gbu operons; oligopeptide permease encoded by oppA; ferritin (Fri); and phosphohydrolase, encoded by pgpH and involved in ppGpp turnover (7,9,20,23,28,41,42,45,46). L. monocytogenes harbors 4 putative DExD-box RNA helicases, and in a previous study, the genes encoding three RNA helicases (lmo0866, lmo1450, and lmo1722) were found to be induced at low temperatures (11).…”