The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer

Zhang, Ya-long; Li, Xuebing; Hou, Yan-xu; Fang, Nianzhen; You, Jiacong; Zhou, Qinghua

doi:10.1038/aps.2016.132x

Cited by 6 publications

(7 citation statements)

References 30 publications

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“…In addition, it was also demonstrated that XIST overexpression enhanced the expression and release of pro-inflammatory mediators in microglia. LncRNA XIST has been reported to participate in multiple diseases, including cancer and neurological diseases (16,(23)(24)(25)(26). In spinal cord injury, XIST promoted neuronal apoptosis by regulating AKT phosphorylation (16).…”

Section: Discussionmentioning

confidence: 99%

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction

Zhang

Yang

2021

Exp Ther Med

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Proinflammatory polarization of microglia aggravates brain injury in cerebral infarction. The present study focused on the role of long non-coding (lnc)RNA X-inactive specific transcript (XIST) in the phenotype modulation of microglia. It was revealed that lncRNA XIST was significantly upregulated in both a mouse cerebral infarction model induced by middle cerebral artery occlusion (MCAO) and an activated microglial model induced by oxygen/glucose deprivation (OGD). The overexpression of XIST enhanced the expression and release of pro-inflammatory mediators [such as tumor necrosis factor (TNF)-α, IL-6, and iNOS] in microglia. Culture supernatant from lncRNA XIST-overexpressed microglial cells induced the apoptosis of primary neurons, while TNF-α antibody counteracted this neurotoxic effect. LncRNA XIST served as a sponge for miR-96-5p, counteracting its inhibitory effect on IKKβ/NF-κB signaling and TNF-α production. Notably, TNF-α was positively regulated by XIST and in turn enhanced XIST expression in microglia. The lncRNA XIST-TNF-α feedback promoted the proinflammatory polarization of microglia, thereby exacerbating cerebral neuron apoptosis.

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Section: Discussionmentioning

confidence: 99%

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction

Zhang

Yang

2021

Exp Ther Med

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“…The activation of SDF-1/CXCR4 axis upregulates lncRNA Xist in rectal cancer [25]. LncRNA Xist is involved in the intimal metastasis and invasion of NSCLC [26,27]. RT-qPCR demonstrated that CAFs increased the levels of CXCR4 and Xist in A549 or H1299 cells, while silencing SDF-1 in CAFs decreased the expressions of CXCR4 and Xist.…”

Section: Discussionmentioning

confidence: 99%

“…The activation of SDF-1/CXCR4 axis can upregulate lncRNA Xist and participate in the occurrence and development of rectal cancer [25]. LncRNA Xist is involved in the intimal metastasis and invasion of NSCLC [26,27]. Therefore, we speculated whether SDF-1 promotes NSCLC by regulating Xist through CXCR4.…”

Section: Sdf-1 Promotes Lncrna Xist Expression Through Cxcr4mentioning

confidence: 99%

Stromal cell-derived factor 1 secreted by cancer-associated fibroblasts promotes lncRNA Xist through CXCR4 and facilitates invasion and metastasis of non-small cell lung cancer

Wang

Chen

et al. 2021

Preprint

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Cancer-associated fibroblasts (CAFs) abundantly exist in NSCLCs and promote tumor progression in most solid tumors. This study investigated the effect of CAFs on NSCLC. CAFs and normal fibroblasts (NFs) were isolated and identified. CAFs or NFs were cocultured with NSCLCs cells. Cell invasion and migration, and expression of E-cadherin, N-cadherin, SDF-1, CXCR4 and Xist were measured. SDF-1/CXCR4 axis inhibitor ADM3100 or low expression of Xist was added to verify the invasion and metastasis of NSCLC. The binding relationships of Xist, miR-15a-5p and MMP3 were analyzed. The effect of miR-15a-5p overexpression on H1299 was evaluated. Finally, lung metastasis experiment was carried out in vivo. CAFs promoted the invasion and EMT of NSCLC cells by secreting SDF-1. CAFs increased CXCR4 and Xist expressions in NSCLC cells. SDF-1 knockdown or ADM3100 decreased CXCR4 and Xist expressions. Silencing Xist reduced the promotion of CAFs on NSCLC. Xist targeted miR-15a-5p to regulate MMP3. Overexpression of miR-15a-5p promoted the invasion and EMT of NSCLC cells. CAFs caused more metastatic nodules and elevated SDF-1, CXCR4, MMP3 and XIST levels, and lowered miR-15a-5p level. Collectively, SDF-1 secreted by CAFs promotes lncRNA Xist through CXCR4, and Xist targets miR-15a-5p to regulate MMP3, thus promoting invasion and metastasis of NSCLC.

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“…They recommended that XIST could be a prognostic factor in NSCLC 141 . Zhang et al identified that XIST has an oncogenic role through controlling of Bcl‐2 and miR‐449a in human NSCLC and XIST upregulation activates cell invasion and metastasis by suppression of miR‐449a 142 …”

Section: Crndementioning

confidence: 99%

Long non‐coding RNAs as potential biomarkers in the prognosis and diagnosis of lung cancer: A review and target analysis

et al. 2020

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Long non-coding RNAs (lncRNA) have been emerged as a novel class of molecular regulators in cancer. They are dysregulated in many types of cancer; however, there is not enough knowledge available on their expression and functional profiles. Lung cancer is the leading cause of the cancer deaths worldwide. Generally, lncRNAs may be associated with lung tumor pathogenesis and they may act as biomarkers for the cancer prognosis and diagnosis. Compared to other invasive prognostic and diagnostic methods, detection of lncRNAs might be a user-friendly and noninvasive method. In this review article, we selected 27 tumor-associated lncRNAs by literature reviewing to further discussing in detail for using as diagnostic and prognostic biomarkers in lung cancer. Also, in an in silico target analysis, the "Experimentally supported functional regulation" approach of the LncTarD web tool was used to identifying the target genes and regulatory mechanisms of the selected lncRNAs. The reports on diagnostic and prognostic potential of all selected lncRNAs were discussed. However, the target genes and regulatory mechanisms of the 22 lncRNAs were identified by in silico analysis and we found the pathways that are controlled by each target group of lncRNAs. They use epigenetic mechanisms, ceRNA mechanisms, protein interaction and sponge mechanism. Also, 10, 23, 5, and 28 target genes for each of these mechanisms were identified, respectively. Finally, each group of target genes controls 50, 12, 7, and 2 molecular pathways, respectively. In conclusion, LncRNAs could be used as

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The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer

Cited by 6 publications

References 30 publications

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction

Stromal cell-derived factor 1 secreted by cancer-associated fibroblasts promotes lncRNA Xist through CXCR4 and facilitates invasion and metastasis of non-small cell lung cancer

Long non‐coding RNAs as potential biomarkers in the prognosis and diagnosis of lung cancer: A review and target analysis

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