The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells

Ou, Farong; Su, Kai; Sun, Jiadong; Liao, Wenting; Yu, Yao; Zheng, Yang; Zhang, Zhiguang

doi:10.1016/j.bbrc.2017.04.090

Cited by 37 publications

(26 citation statements)

References 29 publications

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“… 41 Furthermore, lncRNA ZBED3-AS1 could activate Wnt/β-catenin signaling and stimulate chondrogenesis in human synovial-fluid-derived MSCs. 42 What is more, certain lncRNAs could be regulated by Wnt/β-catenin signaling during osteogenesis differentiation. A putative osteo-suppressing lncRNA HOTAIR was downregulated by Wnt/β-catenin signaling, which led to the upregulation of downstream osteogenic markers ALP and BMP2.…”

Section: Discussionmentioning

confidence: 99%

Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145

Shi

et al. 2018

Molecular Therapy - Nucleic Acids

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Long noncoding RNAs (lncRNAs), which serve as important and powerful regulators of various biological activities, have gained widespread attention in recent years. Emerging evidence has shown that some lncRNAs play important regulatory roles in osteoblast differentiation of mesenchymal stem cells (MSCs), suggesting a potential therapeutic strategy for bone fracture. As a recently identified lncRNA, linc-ROR was reported to mediate the reprogramming ability of differentiated cells into induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs) self-renewal. However, other functions of linc-ROR remain elusive. In this study, linc-ROR was found to be upregulated during osteogenesis of human bone-marrow-derived MSCs. Ectopic expression of linc-ROR significantly accelerated, whereas knockdown of linc-ROR suppressed, osteoblast differentiation. Using bioinformatic prediction and luciferase reporter assays, we demonstrated that linc-ROR functioned as a microRNA (miRNA) sponge for miR-138 and miR-145, both of which were negative regulators of osteogenesis. Further investigations revealed that linc-ROR antagonized the functions of these two miRNAs and led to the de-repression of their shared target ZEB2, which eventually activated Wnt/β-catenin pathway and hence potentiated osteogenesis. Taken together, linc-ROR modulated osteoblast differentiation by acting as a competing endogenous RNA (ceRNA), which may shed light on the functional characterization of lncRNAs in coordinating osteogenesis.

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Section: Discussionmentioning

confidence: 99%

Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145

Shi

et al. 2018

Molecular Therapy - Nucleic Acids

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“…Ou et al studied that the lncRNA expression profile changed during induced cartilage differentiation process in hBMSCs, which suggested that 2166 lncRNAs were upregulated and 1472 lncRNAs were downregulated; the expression of ZBED3-AS1 (ZBED3 antisense RNA 1) and CTA-941F9.9 was verified by Q-PCR. These results indicate that lncRNAs are involved in MSC cartilage differentiation [ 68 ] ( Table 2 ).…”

Section: Lncrnas Involved In Chondrogenic Differentiationmentioning

confidence: 95%

An Overview of Long Noncoding RNAs Involved in Bone Regeneration from Mesenchymal Stem Cells

Peng

Cao

et al. 2018

Stem Cells International

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Bone regeneration is very important for the recovery of some diseases including osteoporosis and bone fracture trauma. It is a multiple-step- and multiple-gene-involved complex process, including the matrix secretion and calcium mineralization by osteoblasts differentiated from mesenchymal stem cells (MSCs) and the absorption of calcium and phosphorus by osteoclasts differentiated from hematopoietic stem cells. Long noncoding RNAs (lncRNAs) are a family of transcripts longer than 200 nt without or with very low protein-coding potential. Recent studies have demonstrated that lncRNAs are widely involved in the regulation of lineage commitment and differentiation of stem cells through multiple mechanisms. In this review, we will summarize the roles and molecular mechanism of lncRNAs including H19, MALAT1, MODR, HOTAIR, DANCR, MEG3, HoxA-AS3, and MIAT in osteogenesis ossification; lncRNA ZBED3-AS1 and CTA-941F9.9, DANCR, and HIT in chondrogenic differentiation; and lncRNA DANCR in osteoclast differentiation. These findings will facilitate the development and application of novel molecular drugs which regulate the balance of bone formation and absorption.

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“…Wang et al [53] demonstrated that the two lncRNAs may function in the early stage of chondrogenic differentiation. Further studies by Ou et al [54] found that ZBED3-AS1 could activate the Wnt/β-catenin signaling and increased the zbed expression. Overexpression of ZBED3-AS1 upregulates the expression levels of sox9 and collagen II, but the detailed mechanism requires further investigation.…”

Section: The Role Of Lncrnas In Cartilage Developmentmentioning

confidence: 95%

lncRNAs: function and mechanism in cartilage development, degeneration, and regeneration

Zhu

Wang

et al. 2019

Stem Cell Res Ther

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With the increasing incidence of cartilage-related diseases such as osteoarthritis (OA) and intervertebral disc degeneration (IDD), heavier financial and social burdens need to be faced. Unfortunately, there is no satisfactory clinical method to target the pathophysiology of cartilage-related diseases. Many gene expressions, signaling pathways, and biomechanical dysregulations were involved in cartilage development, degeneration, and regeneration. However, the underlying mechanism was not clearly understood. Recently, lots of long non-coding RNAs (lncRNAs) were identified in the biological processes, including cartilage development, degeneration, and regeneration. It is clear that lncRNAs were important in regulating gene expression and maintaining chondrocyte phenotypes and homeostasis. In this review, we summarize the recent researches studying lncRNAs' expression and function in cartilage development, degeneration, and regeneration and illustrate the potential mechanism of how they act in the pathologic process. With continued efforts, regulating lncRNA expression in the cartilage regeneration may be a promising biological treatment approach.

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The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells

Cited by 37 publications

References 29 publications

Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145

Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145

An Overview of Long Noncoding RNAs Involved in Bone Regeneration from Mesenchymal Stem Cells

lncRNAs: function and mechanism in cartilage development, degeneration, and regeneration

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