The Localization of [3h]aldosterone and [3h]cortisol Within Renal Tubular Cells by Electron Microscope Autoradiography

Williams, M. A.; Baba, W. I.

doi:10.1677/joe.0.0390543

Cited by 36 publications

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“…The above data are in harmony with indications that selective hor mone accumulation in lysosomes [76,80] and in 'mitochondria' [20,104,108] precedes nuclear uptake (table II). Lysosomes have long been re cognized as contaminants of mitochondrial preparations [15,105].…”

Section: Transnuclear Penetration Of Lysosomal Components Including supporting

confidence: 80%

Steroid-Protein Binding: from Circulating Blood to Target Cell Nucleus

Szego

1976

Gynecol Obstet Invest

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The concept of protein binding of steroid hormones is traced from its origins through the period of confirmation and initial extension. Further considerations include cell surface recognition sites, subcellular sources of binding protein, and the new evidence that lysosomes participate in the translocation and intranuclear penetration of the hormone in steroid target cells. This short review thus aims to provide a link between current investigations and the ideas upon which they have been based.

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Section: Transnuclear Penetration Of Lysosomal Components Including supporting

confidence: 80%

Steroid-Protein Binding: from Circulating Blood to Target Cell Nucleus

Szego

1976

Gynecol Obstet Invest

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“…The finding that delayed stimulation of the Na efflux caused by aldosterone depends rather greatly on the supply of mitochondrial ATP is indirectly supported by the studies of Williams & Baba (1967) who, working with rat kidney, succeeded in demonstrating that mitochondria and plasma membranes are the main binding sites of tritiated aldosterone. Of course, it must be admitted that such an argument, based on an autoradiographic method, is far from water-tight.…”

Section: Discussionmentioning

confidence: 92%

Mode of stimulation by aldosterone of the sodium efflux in barnacle muscle fibres: effects of ouabain, ethacrynic acid, diphenylhydantoin, (ATPMg)(2‐), adenine translocase inhibitors, pyruvate and oxythiamine.

Bittar¹,

Tallitsch²

1976

The Journal of Physiology

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SUMMARY1. A study has been made of the nature of the delayed stimulation caused by external aldosterone in barnacle fibres pre-exposed to aldosterone.2. (i) Microinjection of 0*5 M-ATPMg2-caused only a small but prompt rise in the Na efflux.(ii) Microinjection of 0-5 M-ATPMg2-followed by external application of 10-5 M aldosterone greatly augmented the magnitude of the delayed stimulation. The response was dose-dependent, as well as dependent on the concentration of external K+ and H+, but not Na+, Ca2+ or Mg2+.(iii) External application of 10-5 M aldosterone for 30 min followed by its withdrawal from the bathing medium failed to bring about delayed stimulation. By contrast, fibres into which ATP had been injected showed delayed stimulation under these conditions.3. Microinjection of actinomycin-D or spironolactone SC-14266 into fibres into which ATP had been injected followed by external application of aldosterone resulted in complete abolition of the delayed stimulation.4. Delayed stimulation was reduced whether ATP had been injected or not by prior external application of 10-4 M ouabain or internal application of 8 x 10-2 M ethacrynic acid. It was completely abolished by prior application of ouabain externally and ethacrynic acid internally, or only 104 M diphenylhydantoin externally.5. (i) Microinjection of atractyloside or bongkrekic acid caused a substantial fall in the resting Na efflux. Bongkrekic acid proved more powerful than atractyloside. Microinjection of 0-05 M-ATPMg2-into fibres poisoned with 2-0 x 10-2 M bongkrekic acid completely restored the Na efflux.E. E. BITTAR AND R. B. TALLITSCH Ouabain caused no reduction in the bongkrekic acid-insensitive Na efflux, whereas bongkrekic acid caused a reduction in the ouabaininsensitive Na efflux.(ii) Microinjection of atractyloside or bongkrekic acid prior to external application of aldosterone resulted in complete abolition of the delayed response.6. (i) Microinjection of 10-2 M pyruvate caused a brief small rise in the rate constant for Na efflux and when followed by external application of aldosterone there was an enhanced delayed response.(ii) Microinjection of 10-2 M oxythiamine caused a gradual fall in the resting Na efflux and abolished the delayed stimulation caused by external aldosterone. Fibres into which pyruvate had been injected when injected with oxythiamine behaved towards external aldosterone in the same was as control fibres injected with oxythiamine.7. Microinjection of phosphatidyl-L-serine (20 mg/ml.) before external application of aldosterone failed to result in an enhanced delayed response.8. It is concluded that the delayed response in unenriched and ATPenriched fibres depends on stimulation by aldosterone of new RNA and protein synthesis, and that this induction mechanism leads to an increased activity of two membrane enzyme systems, one ouabain-sensitive and the other ethacrynic acid-sensitive. The argument that the ethacrynic acid-sensitive component of the resting Na efflux and of the aldosteronestimulated Na efflux are closely c...

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“…Each circle was of a diameter such that it could reasonably be assumed to possess 50% probability of enclosing the silver grain formed as a result of a single decay occurring at its centre. This 'circle method' (Williams & Baba, 1967;Williams, 1969) was widely used and helped to introduce a rational approach to image interpretation in this fiela. Variants of the circle approach have also been offered (see the discussion in Salpeter & McHenry, 1973;and Williams, 1977b).…”

Section: The General ('U N R E S T R I C T E D') a P P R O A C Hmentioning

confidence: 99%

Autoradiography: its methodology at the present time

Williams

1982

Journal of Microscopy

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SUMMARY The autoradiographic methods used in biological and medical research are reviewed from an historical viewpoint. It is pointed out that early methodological research was directed at the selection of suitable detector substances for radiation (nuclear emulsions) and at the preparation and application of suitable layers of these detectors to biological samples. On the other hand, later research was more concentrated on aspects of the preparation of the biological components of the specimens. In particular, the retention of biochemicals in the tissue sample was studied in detail. Research in the quantification of the emulsion response to α and β radiation paved the way for certain quantitative applications of autoradiography. This has been followed by the derivation of models for the measurement of image spread and the evolution of image analysis methods which allow refined determinations of the specific radioactivity of fine structures taking into account losses and gains of silver grains by image spread. The sum of all these advances is a methodology capable of localizing and quantifying radioactivity and hence marking chemical processes from an organism and organ level down almost to the present limits of anatomical delineation.

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The Localization of [3h]aldosterone and [3h]cortisol Within Renal Tubular Cells by Electron Microscope Autoradiography

Cited by 36 publications

References 0 publications

Steroid-Protein Binding: from Circulating Blood to Target Cell Nucleus

Steroid-Protein Binding: from Circulating Blood to Target Cell Nucleus

Mode of stimulation by aldosterone of the sodium efflux in barnacle muscle fibres: effects of ouabain, ethacrynic acid, diphenylhydantoin, (ATPMg)(2‐), adenine translocase inhibitors, pyruvate and oxythiamine.

Autoradiography: its methodology at the present time

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