Accuracy of temperature measurement of natural gas flows in closed conduits is a highly debated topic due to the complexity of the measurement chain and the related economic impact. First, specific thermo-fluid dynamic issues occur because of the difference between the temperature of the gas stream and that of the external ambient and the mean radiant temperature inside the pipe. Furthermore, the installation conditions of the temperature sensor (e.g., immersion length and diameter of the thermowell) play a crucial role. In this paper, the authors present the results of a numerical and experimental study conducted both in the laboratory and in-field aimed at analyzing the reliability of temperature measurement in natural gas networks as a function of the pipe temperature and of the pressure and velocity of the gas stream. The results obtained in the laboratory show errors ranging between 0.16 and 5.87 °C in the summer regime and between −0.11 and −2.72 °C in the winter regime, depending on the external pipe temperature and gas velocity. These errors have been found to be consistent with those measured in-field, where high correlation between the pipe temperatures, the gas stream and the external ambient have been also demonstrated, especially in summer conditions.