The long and winding road of progress in the use of in vitro data for risk assessment purposes: From “carnation test” to integrated testing strategies

Blaauboer, Bas J.

doi:10.1016/j.tox.2014.04.004

Cited by 15 publications

(6 citation statements)

References 36 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although tremendous progress has been achieved in adapting and validating in vitro tools to environmental monitoring and risk assessment (e.g., in Australasia, Coleman et al 2008;Mispagel et al 2009;Chinathamby et al 2013;Bain et al 2014;Escher et al 2014;Leusch et al 2014;Scott et al 2014;Roberts et al 2015;Boehler et al 2017;Neale, Achard et al 2017;Neale, Altenburger et al 2017;Chen et al 2018;Leusch et al 2018), some fundamental questions still need to be systematically addressed before these techniques can become reliable predictors of whole animal level effects: 1) Refine quantitative in vitro to in vivo extrapolation (QI-VIVE): Although there is a clear correlation between in vitro response and in vivo effects for some endpoints such as acute toxicity (Kaiser 1998;Tanneberger et al 2013;Natsch et al 2018) and receptor-mediated endocrine effects (Sonneveld et al 2006(Sonneveld et al , 2011Henneberg et al 2014), toxicokinetic factors (absorption, distribution, metabolism, and excretion) still pose a difficult challenge for QIVIVE (Blaauboer 2015;Meek and Lipscomb 2015), although groundbreaking studies suggest that this may soon be within reach (Rotroff et al 2010;Wetmore 2015). 2) Fully map relevant AOPs: There is still much work to be done to map key events (KEs) to connect the dots between the molecular or cellular initiating event and the ultimate apical consequence to produce comprehensive AOPs, for both humans and ecosystems ).…”

Section: Tools For Improving Risk Assessmentmentioning

confidence: 99%

Towards Sustainable Environmental Quality: Priority Research Questions for the Australasian Region of Oceania

Gaw

Harford

Pettigrove

et al. 2019

Integr Envir Assess & Manag

View full text Add to dashboard Cite

Environmental challenges persist across the world, including the Australasian region of Oceania, where biodiversity hotspots and unique ecosystems such as the Great Barrier Reef are common. These systems are routinely affected by multiple stressors from anthropogenic activities, and increasingly influenced by global megatrends (e.g., the food–energy–water nexus, demographic transitions to cities) and climate change. Here we report priority research questions from the Global Horizon Scanning Project, which aimed to identify, prioritize, and advance environmental quality research needs from an Australasian perspective, within a global context. We employed a transparent and inclusive process of soliciting key questions from Australasian members of the Society of Environmental Toxicology and Chemistry. Following submission of 78 questions, 20 priority research questions were identified during an expert workshop in Nelson, New Zealand. These research questions covered a range of issues of global relevance, including research needed to more closely integrate ecotoxicology and ecology for the protection of ecosystems, increase flexibility for prioritizing chemical substances currently in commerce, understand the impacts of complex mixtures and multiple stressors, and define environmental quality and ecosystem integrity of temporary waters. Some questions have specific relevance to Australasia, particularly the uncertainties associated with using toxicity data from exotic species to protect unique indigenous species. Several related priority questions deal with the theme of how widely international ecotoxicological data and databases can be applied to regional ecosystems. Other timely questions, which focus on improving predictive chemistry and toxicology tools and techniques, will be important to answer several of the priority questions identified here. Another important question raised was how to protect local cultural and social values and maintain indigenous engagement during problem formulation and identification of ecosystem protection goals. Addressing these questions will be challenging, but doing so promises to advance environmental sustainability in Oceania and globally.

show abstract

Section: Tools For Improving Risk Assessmentmentioning

confidence: 99%

Towards Sustainable Environmental Quality: Priority Research Questions for the Australasian Region of Oceania

Gaw

Harford

Pettigrove

et al. 2019

Integr Envir Assess & Manag

View full text Add to dashboard Cite

show abstract

“…But their applications have been limited (Sobanska et al 2014 ). There have been many debates about the limitations of in vitro assay systems and the lack of proper accounting for in vitro toxicokinetic testing (Blaauboer 2015 ). This argument overlooks scientific progress rooting HTS assays in adverse outcome pathways (Burden et al 2015 ) and unravelling the biokinetics of in vitro assays (Fischer et al 2018 ; Proença et al 2021 ) that strengthen the scientific basis for the use of HTS assays in risk assessment.…”

Section: Introductionmentioning

confidence: 99%

Modernizing persistence–bioaccumulation–toxicity (PBT) assessment with high throughput animal-free methods

et al. 2023

View full text Add to dashboard Cite

The assessment of persistence (P), bioaccumulation (B), and toxicity (T) of a chemical is a crucial first step at ensuring chemical safety and is a cornerstone of the European Union’s chemicals regulation REACH (Registration, Evaluation, Authorization, and Restriction of Chemicals). Existing methods for PBT assessment are overly complex and cumbersome, have produced incorrect conclusions, and rely heavily on animal-intensive testing. We explore how new-approach methodologies (NAMs) can overcome the limitations of current PBT assessment. We propose two innovative hazard indicators, termed cumulative toxicity equivalents (CTE) and persistent toxicity equivalents (PTE). Together they are intended to replace existing PBT indicators and can also accommodate the emerging concept of PMT (where M stands for mobility). The proposed “toxicity equivalents” can be measured with high throughput in vitro bioassays. CTE refers to the toxic effects measured directly in any given sample, including single chemicals, substitution products, or mixtures. PTE is the equivalent measure of cumulative toxicity equivalents measured after simulated environmental degradation of the sample. With an appropriate panel of animal-free or alternative in vitro bioassays, CTE and PTE comprise key environmental and human health hazard indicators. CTE and PTE do not require analytical identification of transformation products and mixture components but instead prompt two key questions: is the chemical or mixture toxic, and is this toxicity persistent or can it be attenuated by environmental degradation? Taken together, the proposed hazard indicators CTE and PTE have the potential to integrate P, B/M and T assessment into one high-throughput experimental workflow that sidesteps the need for analytical measurements and will support the Chemicals Strategy for Sustainability of the European Union.

show abstract

“…20,21 Cell lines can be a powerful method to study mechanisms of toxicity and potential species differences. 22 Cell lines have been established and successfully applied in toxicity testing for several cetaceans, including a number of Mediterranean dolphin species and Arctic beluga whales. 23,24 Recently, we developed a humpback whale broblast cell line (HuWa), for the rst time opening up the possibility for in vitro effect assessment for this species.…”

Section: Introductionmentioning

confidence: 99%

Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions

et al. 2019

View full text Add to dashboard Cite

show abstract

The long and winding road of progress in the use of in vitro data for risk assessment purposes: From “carnation test” to integrated testing strategies

Cited by 15 publications

References 36 publications

Towards Sustainable Environmental Quality: Priority Research Questions for the Australasian Region of Oceania

Towards Sustainable Environmental Quality: Priority Research Questions for the Australasian Region of Oceania

Modernizing persistence–bioaccumulation–toxicity (PBT) assessment with high throughput animal-free methods

Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions

Contact Info

Product

Resources

About