The Long Non-Coding RNA LncRNA8975-1 is Upregulated in Hypertrophic Scar Fibroblasts and Controls Collagen Expression

Li, Jun; Chen, Ling; Cao, Chunyu; Yan, Hui; Zhou, Bei; Gao, Yanli; Li, Qian; Li, Jingyun

doi:10.1159/000452548

Cited by 33 publications

(41 citation statements)

References 36 publications

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“…Increase in the biosynthesis of fibroblasts cause excessive collagen deposition of extracellular matrix (25,26). Collagen fibers also form here as a result of the body synthesizing collagen at an increased rate compared with that at which it catabolizes collagen over a longer period of time (27,28).…”

Section: Discussionmentioning

confidence: 99%

Retrospective observational cohort study on cosmetic outcome of using Ti‑Ni memory alloy wire for intradermal suture following mastectomy in patients with breast cancer

Qin

Sun

et al. 2017

Oncol Lett

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Abstract. The method of suturing for incisions is crucial for the comprehensive treatment of clinical patients with breast cancer. Suturing is considered a major part of post-surgical recovery and may serve as a marker for evaluation of surgical outcome. The present study aimed to establish an effective means of suturing for patients who received modified radical surgery that helps to improve the cosmetic outcome of the incision. Enrolled patients were divided into an active and a control group. Ti-Ni memory alloy wire for intradermal suture in the active group and silk for interruption suture in the control group were applied to assess the different prognosis-associated factors. The Vancouver Scar Scale (VSS) was used to evaluate the wound size and the recovery time of the scars. The association between diabetes and the number of days of wound healing was also analyzed. The results indicated that the mean VSS score of the active group was decreased compared with that of the control group (P<0.001). The VSS scores of four main features (vascularity, pigmentation, pliability and height) between the two groups also statistically differed (P<0.001). Furthermore, the mean number of days of wound healing was significantly decreased for the active group compared with that for the control group (P=0.0026) in the patients with diabetes. In addition, the usage of Ti-Ni memory alloy wire was able to decrease the mean number of wound healing days between patients with diabetes and their non-diabetic counterparts (P=0.7009). The present study indicated that intradermal suture offers improved cosmetic outcome for patients undergoing mastectomy with or without axillary surgery. This technique may be useful for preventing scar overgrowth and for facilitating the recovery process in patients with diabetes. IntroductionIn 1882, Halsted pioneered the radical resection of the breast. Breast surgery methods have changed since and clinicians commonly perform breast-conserving surgery. However, the optimal approach for radical resection remains controversial (1). Along with social progress and improvements in the quality of life, patients with cancer, particularly patients with breast cancer have increasing requirements for the function and cosmetic outcome following surgery (2,3). A previous study identified that 61% of patients reported that the opinions of their partners of the scars were important to them (4). The way an incision is closed and managed post-operatively affects cosmetic outcome (5). Historically, in breast cancer surgery, simultaneously ensuring treatment effect, shrinking excision scope and attending to the cosmetic effect has been important (6). To improve the aesthetic results for female patients undergoing breast cancer surgery, surgical techniques have been developed to render scars less noticeable (7,8).In China, the majority of patients with breast cancer are treated with modified radical surgery using discontinuous silk suture, which leaves a very noticeable scar (9). A study on cosmetic surgery identified ...

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Section: Discussionmentioning

confidence: 99%

Retrospective observational cohort study on cosmetic outcome of using Ti‑Ni memory alloy wire for intradermal suture following mastectomy in patients with breast cancer

Qin

Sun

et al. 2017

Oncol Lett

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“…Cultures of normal skin fibroblasts (NSFs) were established as previously described [12]. Explants were cultured in DMEM (Invitrogen, USA) supplemented with 10% foetal bovine serum, 100 µg/mL streptomycin and 100 U/mL penicillin at 37°C in 5% CO 2 .…”

Section: Cell Culture and Tgf-β1 Treatmentmentioning

confidence: 99%

“…Quantitative reverse transcription PCR Total RNA was isolated from each tissue and corresponding cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) as we previously described [12]. Transcript levels for AC067945.2 were normalized to GAPDH cDNA levels using the 2 (-DDCt) method.…”

Section: Cell Culture and Tgf-β1 Treatmentmentioning

confidence: 99%

“…The protocol was the same as we previously reported [12] . Briefly, proteins (15 μg) were separated by SDS-PAGE and transferred to nitrocellulose membranes (Amersham, Chalfont, UK).…”

Section: Western Blotmentioning

confidence: 99%

“…By regulating gene expression, lncRNAs play crucial roles in multiple biological processes such as development, differentiation and carcinogenesis [8][9][10][11]. Specific lncRNAs are correlated with fibrotic disorders including hypertrophic scar [12], keloid [13], and lung fibrosis [14], indicating that lncRNAs may be involved in imbalances in ECM deposition. Recently, our group discovered that AC067945.2 (UCSC hg38; also called NONHSAT076109 in NONCODEv4 and HG495060 in GenBank), a lncRNA located upstream of the STAT1 gene on chromosome 2 (UCSC hg38, chr2:191017954-191019079), is dysregulated in regressive scars compared to that in mature scar tissues [15].…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of LncRNA AC067945.2 Down-Regulates Collagen Expression in Skin Fibroblasts and Possibly Correlates with the VEGF and Wnt Signalling Pathways

Chen¹,

Li²,

Li³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Long non-coding RNAs (lncRNAs) are thought to play crucial roles in human diseases. However, the function of lncRNAs in hypertrophic scar formation remains poorly understood. Methods: Utilizing qRT-PCR, we explored the expression changes of AC067945.2. Overexpression of AC067945.2 in normal skin fibroblasts was performed by transient plasmid transfection. Western blot was used to check the proteins' expression changes. Cell Counting Kit-8 (CCK-8) assay and Annexin V/7-AAD staining were used to examine cell proliferation and apoptosis, respectively. mRNA-seq was applied to dissect the differentially expressed mRNAs in AC067945.2 overexpressed cells. We also performed ELISA to detect the VEGF secretion. Results: AC067945.2 was down-regulated in hypertrophic scar tissues. Overexpression of AC067945.2 did not affect cell proliferation, but it mildly promoted early apoptosis in normal skin fibroblasts. Furthermore, AC067945.2 overexpression inhibited the expression of COL1A1, COL1A2, COL3A1 and α-SMA proteins. Transforming growth factor-β1 (TGF-β1) could inhibit the expression of AC067945.2. Based on mRNA-seq data, compared with mRNAs in the control group, 138 mRNAs were differentially expressed, including 14 upregulated and 124 down-regulated transcripts, in the AC067945.2 overexpression group. Gene ontology and pathway analyses revealed that AC067945.2 overexpression was correlated with developmental processes, binding, extracellular region, and the vascular endothelial

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Identification of crucial noncoding RNAs and mRNAs in hypertrophic scars via RNA sequencing

He²,

Zhang³

et al. 2021

FEBS Open Bio

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Hypertrophic scaring (HS) is a dermal fibroproliferative disorder characterized by excessive deposition of collagen and other extracellular matrix (ECM) components. The aim of this study is to explore crucial lncRNAs and circRNAs associated with HS and provide a better understanding of the molecular mechanism of HS. To investigate the lncRNA, circRNA and mRNA expression profiles we performed RNA-sequencing of human HS and normal skin tissues. Following the identification of differentially expressed mRNAs (DEmRNAs), lncRNAs (DElncRNAs) and circRNAs (DEcircRNAs), we performed functional enrichment of DEmRNAs. Further on, we constructed DElncRNA/DEcircRNA-DEmRNA co-expression networks and ceRNA regulatory networks and performed functional analyses of the DEmRNAs in the constructed networks. In total, 487 DEmRNAs, 92 DElncRNAs and 17 DEcircRNAs were identified. DEmRNAs were significantly enriched in processes like collagen fibril organization, ECM-receptor interaction and PI3K-Akt signaling pathway. Additionally, we detected 580 DElncRNA-DEmRNA and 505 DEcircRNA-DEmRNA co-expression pairs The ceRNA network contained 18 circRNA-miRNA pairs, 18 lncRNA-miRNA pairs and 409 miRNA-mRNA pairs, including 10 circRNAs, 5 lncRNAs, 15 miRNAs, and 160 mRNAs. We concluded that MIR503HG/hsa-miR-204-3p/ACAN, MIR503HG/hsa-miR-431-5p/TNFRSF9, MEG3/hsa-miR-6884-5p/ADAMTS14, AC000035.1-ADAMTS14 and hsa_circ_0069865-COMP/ADAM12 interaction pairs may play a central role in HS.

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The Long Non-Coding RNA LncRNA8975-1 is Upregulated in Hypertrophic Scar Fibroblasts and Controls Collagen Expression

Cited by 33 publications

References 36 publications

Retrospective observational cohort study on cosmetic outcome of using Ti‑Ni memory alloy wire for intradermal suture following mastectomy in patients with breast cancer

Retrospective observational cohort study on cosmetic outcome of using Ti‑Ni memory alloy wire for intradermal suture following mastectomy in patients with breast cancer

Overexpression of LncRNA AC067945.2 Down-Regulates Collagen Expression in Skin Fibroblasts and Possibly Correlates with the VEGF and Wnt Signalling Pathways

Identification of crucial noncoding RNAs and mRNAs in hypertrophic scars via RNA sequencing

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