The long-term survival of <i>Propionibacterium freudenreichii</i>
 in a context of nutrient shortage

Aburjaile, Flávia Figueira; Madec, Marie‐Noëlle; Parayre, Sandrine; Miyoshi, Anderson; Azevedo, Vasco; Loir, Yves Le; Falentin, Hélène

doi:10.1111/jam.13000

Cited by 13 publications

(12 citation statements)

References 40 publications

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“…Sequencing projects resulted in 22 draft genomes [ 23 , 24 ] and two additional complete genomes [ 25 , 26 ] available for the species. Although the draft genomes proved valuable and were used in a number of comparative and functional studies [ 24 , 27 – 29 ], they do not permit studies of genome organization or mobile elements absent from the reference genome [ 30 ]. In addition, due to the nature of the short-read sequencing itself, draft genomes do not give an insight into additional regions of sequences rich in repeats such as CRISPR-Cas systems, transposed mobile elements or gene duplications [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii

et al. 2017

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Background Propionibacterium freudenreichii is an industrially important bacterium granted the Generally Recognized as Safe (the GRAS) status, due to its long safe use in food bioprocesses. Despite the recognized role in the food industry and in the production of vitamin B12, as well as its documented health-promoting potential, P. freudenreichii remained poorly characterised at the genomic level. At present, only three complete genome sequences are available for the species.ResultsWe used the PacBio RS II sequencing platform to generate complete genomes of 20 P. freudenreichii strains and compared them in detail. Comparative analyses revealed both sequence conservation and genome organisational diversity among the strains. Assembly from long reads resulted in the discovery of additional circular elements: two putative conjugative plasmids and three active, lysogenic bacteriophages. It also permitted characterisation of the CRISPR-Cas systems. The use of the PacBio sequencing platform allowed identification of DNA modifications, which in turn allowed characterisation of the restriction-modification systems together with their recognition motifs. The observed genomic differences suggested strain variation in surface piliation and specific mucus binding, which were validated by experimental studies. The phenotypic characterisation displayed large diversity between the strains in ability to utilise a range of carbohydrates, to grow at unfavourable conditions and to form a biofilm.ConclusionThe complete genome sequencing allowed detailed characterisation of the industrially important species, P. freudenreichii by facilitating the discovery of previously unknown features. The results presented here lay a solid foundation for future genetic and functional genomic investigations of this actinobacterial species.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4165-9) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii

et al. 2017

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“…The discovery of its probiotic and bifidogenic properties (Kaneko et al, 1994; Mantere-Alhonen, 1995) lead to increased number of studies focusing on the species. As a result, the first genome sequence of the P. freudenreichii CIRM-BIA1 T was published (Falentin et al, 2010) and then followed by further studies providing deeper insight into the adaptation mechanisms and long-term survival of this species (Thierry et al, 2011; Dalmasso et al, 2012a; Aburjaile et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Food-Like Growth Conditions Support Production of Active Vitamin B12 by Propionibacterium freudenreichii 2067 without DMBI, the Lower Ligand Base, or Cobalt Supplementation

et al. 2017

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Propionibacterium freudenreichii is a traditional dairy bacterium and a producer of short chain fatty acids (propionic and acetic acids) as well as vitamin B12. In food applications, it is a promising organism for in situ fortification with B12 vitamin since it is generally recognized as safe (GRAS) and it is able to synthesize biologically active form of the vitamin. In the present study, vitamin B12 and pseudovitamin biosynthesis by P. freudenreichii was monitored by UHPLC as a function of growth in food-like conditions using a medium mimicking cheese environment, without cobalt or 5,6-dimethylbenzimidazole (DMBI) supplementation. Parallel growth experiments were performed in industrial-type medium known to support the biosynthesis of vitamin B12. The production of other key metabolites in the two media were determined by HPLC, while the global protein production was compared by gel-based proteomics to assess the effect of growth conditions on the physiological status of the strain and on the synthesis of different forms of vitamin. The results revealed distinct protein and metabolite production, which reflected the growth conditions and the potential of P. freudenreichii for synthesizing nutritionally relevant amounts of active vitamin B12 regardless of the metabolic state of the cells.

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“…The emergence of functional foods for improving the survival of therapeutic dairy origin probiotics, such as wild-type and genetically improved strains, is a promising research area. Depending on the food matrix biochemical, it may protect bacteria towards digestive stresses, whereas the fermented dairy products confer a high amount of essential nutrient for consumer [63,64]. In this context, cheese matrices have been explored for probiotic strains of Lactobacillus spp.…”

Section: Protection Matricesmentioning

confidence: 99%

Wild-Type and Genetically Improved Strains of Dairy Origin Probiotic as Potential Treatments for Intestinal Mucositis

Aburjaile¹,

Jan²,

Loir³

et al. 2019

JPP

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The use of probiotic bacteria derived from fermented foods has been explored by the scientific community as alternative strategies for the treatment of several diseases, mainly regarding intestinal dysfunction. One of the most relevant inflammatory diseases affecting the alimentary tract, for which no current intervention is entirely successful, is mucositis. In this review article, we summarize the most recent proof-of-concept studies dealing with the therapeutic use of dairy origin probiotics, for the treatment of gastrointestinal mucositis. Furthermore, we discuss several approaches for the improvement of the classical therapeutic rationale, such as supplementation with prebiotics and genetic engineering along with the respective translational issues, which may be crucial for the successful transposition of these therapeutic strategies for clinical use.

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The long-term survival of Propionibacterium freudenreichii in a context of nutrient shortage

Abstract: Assessing the viability of P. freudenreichii and understanding their mechanisms for survival should be of great interest regarding their potential probiotic applications.

Cited by 13 publications

References 40 publications

De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii

De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii

Food-Like Growth Conditions Support Production of Active Vitamin B12 by Propionibacterium freudenreichii 2067 without DMBI, the Lower Ligand Base, or Cobalt Supplementation

Wild-Type and Genetically Improved Strains of Dairy Origin Probiotic as Potential Treatments for Intestinal Mucositis

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