The Lyme disease vaccine candidate outer surface protein A (OspA) in a formulation compatible with human use protects mice against natural tick transmission of B. burgdorferi

Golde, William T.; Burkot, Thomas R.; Piesman, Joseph; Dolan, Marc C.; Capiau, Carine; Hauser, P; Dequesne, Guy; Lobet, Yves

doi:10.1016/0264-410x(94)00027-k

Cited by 27 publications

(16 citation statements)

References 27 publications

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“…Both proteins are protected from the immune system by an outer membrane whose major components include OspA and OspB in the tick and OspC and OspD in mammals (22). When mice are immunized with a recombinant OspA, they are protected from challenge by the same strain of B. burgdorferi (11,23) but not necessarily from challenge by heterologous strains of B. burgdorferi (12,13).…”

mentioning

confidence: 99%

Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single-strand conformation polymorphism analysis

Guttman

Wang

et al. 1996

J Clin Microbiol

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The genetic heterogeneity of the spirochete Borrelia burgdorferi within single adult black-legged ticks from Shelter Island, N.Y., was determined by cold, single-strand conformation polymorphism (SSCP) analysis. The central region of the ospA gene of B. burgdorferi from infected ticks was amplified by nested PCR. Amplified product of the correct size was obtained from 20 of 45 ticks (44%). This is the fraction of ticks that is expected to be infected with B. burgdorferi. Four variant classes were determined by SSCP analysis. Eight ticks were infected with a single variant, nine ticks were infected with two variants, two ticks were infected with three variants, and one tick was infected with all four variants. DNA from each variant was sequenced. Five different sequences were found. The sequence of each variant was different from that of another variant by a single base. SSCP analysis could distinguish three of the four single-base changes found in the region.

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mentioning

confidence: 99%

Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single-strand conformation polymorphism analysis

Guttman

Wang

et al. 1996

J Clin Microbiol

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“…Of these Osps, OspA and OspC have been most studied. Experimental vaccines made against either of these Osps are successful in warding off challenges from the same strain [20][21][22][23]; however, a number of investigators have found that antisera raised to 1 strain do not necessarily protect against heterologous challenge [23][24][25]. Antisera raised to OspA and OspB confer protection in SCID mice [21].…”

Section: Discussionmentioning

confidence: 99%

Approaches toward the Directed Design of a Vaccine againstBorrelia burgdorferi

Luft¹,

Dunn²,

Lawson³

2002

J INFECT DIS

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The overall efficacy of a recombinant vaccine for Lyme disease that is effective worldwide will depend upon the selection of one or more immunoprotective target(s) and the frequency of genetic variation, which can alter the antigenicity of the immunoprotective epitopes of the target proteins. Careful delineation of these protective epitopes on target antigens is essential for the development of vaccine candidates as well as for understanding the limitations of such vaccines. Structural models of these targets will provide critical information about conformation and specific residue surface accessibility for defining protective epitopes. Co-crystal structures with Fab fragments of protective antibodies will further delineate critical antigen surfaces. Population genetics will provide vital information on the heterogeneity of these proteins. Detailed epitope mapping will provide the information needed for the bioengineering of antigens needed to expand the specificity of a candidate vaccine.

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“…The rOspA vaccine was then shown to be protective against naturally occurring tick-transmitted infection. [189][190][191] The effectiveness of rOspA vaccine initially was surprising because organisms isolated from infected animals do not express OspA until naturally occurring late infections. It was discovered that the vaccine acts primarily in the tick midgut rather than in the vaccinated host.…”

Section: Bacteriamentioning

confidence: 98%

Vaccines and immunotherapies for the prevention of infectious diseases having cutaneous manifestations

Huang

Pang

et al. 2004

Journal of the American Academy of Dermatology

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The Lyme disease vaccine candidate outer surface protein A (OspA) in a formulation compatible with human use protects mice against natural tick transmission of B. burgdorferi

Cited by 27 publications

References 27 publications

Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single-strand conformation polymorphism analysis

Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single-strand conformation polymorphism analysis

Approaches toward the Directed Design of a Vaccine againstBorrelia burgdorferi

Vaccines and immunotherapies for the prevention of infectious diseases having cutaneous manifestations

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