The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing.

Noguchi, T.; Inoue, H.; Tanaka, Takashi

doi:10.1016/s0021-9258(18)67091-7

Cited by 485 publications

(106 citation statements)

References 36 publications

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“…PKM1 and PKM2 arise through alternative splicing (exon 9 for PKM1 and exon 10 for PKM2) and differ in only 23 amino acids. 84,85 PKM2 is the predominant splice variant during brain development in mice and humans, while the major isoform in the adult brain is PKM1. 42 PKM1 forms a highly active tetramer in normal cells and favors glycolysis coupled with oxidative phosphorylation and generating of ATP.…”

Section: The Role Of Pkm2 In Glioblastoma and Its Impact On Epigeneticsmentioning

confidence: 99%

Metabolic reprogramming in glioblastoma: the influence of cancer metabolism on epigenetics and unanswered questions

2015

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A defining hallmark of glioblastoma is altered tumor metabolism. The metabolic shift towards aerobic glycolysis with reprogramming of mitochondrial oxidative phosphorylation, regardless of oxygen availability, is a phenomenon known as the Warburg effect. In addition to the Warburg effect, glioblastoma tumor cells also utilize the tricarboxylic acid cycle/oxidative phosphorylation in a different capacity than normal tissue. Altered metabolic enzymes and their metabolites are oncogenic and not simply a product of tumor proliferation. Here we highlight the advantages of why tumor cells, including glioblastoma cells, require metabolic reprogramming and how tumor metabolism can converge on tumor epigenetics and unanswered questions in the field.

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Section: The Role Of Pkm2 In Glioblastoma and Its Impact On Epigeneticsmentioning

confidence: 99%

Metabolic reprogramming in glioblastoma: the influence of cancer metabolism on epigenetics and unanswered questions

2015

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“…Lactate dehydrogenase A (LDHA) catalyzes the interconversion of pyruvate and lactate [Jiang et al, 2015]. Pyruvate kinase M1 (PKM1) promotes oxidative phosphorylation, whereas pyruvate kinase M2 (PKM2) primarily contributes to aerobic glycolysis [Noguchi et al, 1986]. Isocitrate dehydrogenase 1 (IDH1) participates in normal oxidative reaction [Grassian et al, 2014].…”

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confidence: 99%

LncRNA‐TP53TG1 Participated in the Stress Response Under Glucose Deprivation in Glioma

Chen

Gao

et al. 2017

J of Cellular Biochemistry

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Gliomas are the most common brain tumors of the center nervous system. And long non-coding RNAs (lncRNAs) are non-protein coding transcripts, which have been considered as one type of gene expression regulator for cancer development. In this study, we investigated the role of lncRNA-TP53TG1 in response to glucose deprivation in human gliomas. The expression levels of TP53TG1 in glioma tissues and cells were analyzed by qRT-PCR. In addition, the influence of TP53TG1 on glucose metabolism related genes at the mRNA level during both high and low glucose treatment was detected by qRT-PCR. MTT, clonogenicity assays, and flow cytometry were performed to detect the cell proliferation and cell apoptosis. Furthermore, the migration of glioma cells was examined by Transwell assays. The expression of TP53TG1 was significantly higher in human glioma tissues or cell lines compared with normal brain tissue or NHA. Moreover, TP53TG1 and some tumor glucose metabolism related genes, such as GRP78, LDHA, and IDH1 were up-regulated significantly in U87 and LN18 cells under glucose deprivation. In addition, knockdown of TP53TG1 decreased cell proliferation and migration and down-regulated GRP78 and IDH1 expression levels and up-regulated PKM2 levels in U87 cells under glucose deprivation. However, over-expression of TP53TG1 showed the opposite tendency. Moreover, the effects of TP53TG1 were more remarkable in low glucose than that in high glucose. Our data showed that TP53TG1 under glucose deprivation may promote cell proliferation and migration by influencing the expression of glucose metabolism related genes in glioma. J. Cell. Biochem. 118: 4897-4904, 2017. © 2017 Wiley Periodicals, Inc.

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“…They have different rst exons and are de ned by tissue-speci c promoters [8] . The PKM consists of 12 exons, in which exons 9 and 10 are alternately spliced in mutually exclusive ways to produce PKM1 and PKM2 isoforms, respectively [9] . PKM2 is mainly expressed in a range of cancer cells, as well as in fetal and undifferentiated adult tissues, whereas PKM1 is predominantly expressed in terminally differentiated tissues [10] .…”

Section: Introductionmentioning

confidence: 99%

Elevating PKM2 Expression Indicates a Biomarker of Poor Prognosis in Patients With Liver Cancer

Zhang

et al. 2021

Preprint

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M2 isoform of pyruvate kinase (PKM2) plays an important role in reprogramming of cell metabolism which is a hall-marker of tumorigenesis. PKM2 expression altering is closely related to cancer metabolism and tumor growth. In the present study, we analyzed the role of PKM2 expression in liver cancer in order to clarify its potential application value in the diagnosis and prognosis of liver cancer patients. In cancerous liver tissues, the PKM2 expression was significantly higher than normal tissues. High PKM2 expressing was related to patient’s age, gender, histological type, grade, stage, T classification and poor survival. Patients with Higher PKM2 expression had a shorter OS (P = 0.0013) and RFS (P = 0.027). ROC and Multivariate Cox analysis showed that high PKM2expression was a risk factor for patients’ poor prognosis. GSEA identified mitotic spindle, PI3K/Akt/mTOR signaling, notch signaling, apoptosis, G2M checkpoint and Wnt/β- Cantenin signaling were enriched with high PKM2 expression phenotype. These findings suggested PKM2 expression has potential as a predictive biomarker for the diagnosis and prognosis of patients with liver cancer.

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The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing.

Cited by 485 publications

References 36 publications

Metabolic reprogramming in glioblastoma: the influence of cancer metabolism on epigenetics and unanswered questions

Metabolic reprogramming in glioblastoma: the influence of cancer metabolism on epigenetics and unanswered questions

LncRNA‐TP53TG1 Participated in the Stress Response Under Glucose Deprivation in Glioma

Elevating PKM2 Expression Indicates a Biomarker of Poor Prognosis in Patients With Liver Cancer

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