The macromolecular basis of the hydraulic conductivity of the arterial hall

Kh, Parker; Cp, Winlove

doi:10.3233/bir-1984-211-221

Cited by 22 publications

(15 citation statements)

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“…Indeed the discrepancy for many tissues is an order of magnitude or more, and the view that interstitial GAG alone accounts adequately for interstial resistance does not seem quantitatively justifiable. This conclusion has been reached independently by three groups recently, namely Jackson & James (1982), Parker & Winlove (1984) and Knight & Levick (1985).…”

Section: Conductivity Of Glycosaminoglycan Matrix In Vitromentioning

confidence: 90%

“…The results of method (b) differ considerably, partly because flow proves to be time dependent. Method (b) over-estimates K' initially due to solvent and matrix moving together, upstream from the restraining membrane (A. G. Ogston, personal communication on results of Ogston & Sherman, 1961), while at later times marked concentration polarization can develop down-stream at the matrix-retaining membrane (Parker & Winlove, 1984). The ultracentrifugal sedimentation method seems free from such problems (Ethier, 1986).…”

Section: Conductivity Of Glycosaminoglycan Matrix In Vitromentioning

confidence: 99%

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Flow Through Interstitium and Other Fibrous Matrices

1987

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This review examined the relation between interstitial hydraulic permeability and chemical composition, using porous matrix theory, and assessed the view that interstitial conductivity is governed by GAG concentration. Conductivity correlates negatively with both GAG and collagen concentration over a wide variety of tissues. Conductivities of GAG matrices in vitro, coupled with other quantitative considerations, indicate, however, that no single class of fixed fibrous element exists at a sufficiently high concentration to account by itself for the low conductivity in most tissues. It seems that the low interstitial conductivity arises from the combined interactive effects of three main classes of fibrous element: collagen fibrils, GAG and proteoglycan core protein. In most cases the proteoglycan complex plays a major role which is significantly amplified, however, by the collagen fibril network.

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Section: Conductivity Of Glycosaminoglycan Matrix In Vitromentioning

confidence: 90%

Section: Conductivity Of Glycosaminoglycan Matrix In Vitromentioning

confidence: 99%

Flow Through Interstitium and Other Fibrous Matrices

1987

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“…It was not possible, unfortunately, to reproduce this observation at the higher concentrations owing to the tendency of viscosity to decay with time (see Methods). (iv) Studies in vitro in which hyaluronan solutions are filtered through membranes show that even when pore size is as large as 450 nm, a concentration polarization layer forms at the surface in the presence of endogenous or Sigma grade III hyaluronan (Ogston & Shermann, 1961;Nettelbladt & Sundblad, 1967;Fraser, Murdoch, Curtain & Watt, 1977;Parker & Winlove, 1984;Johnson et at. 1987).…”

Section: Results In Vivo Flow Versus Time Curvesmentioning

confidence: 99%

“…However, gels formed by fitration of hyaluronan solutions across membranes in vitro do indeed have a high hydraulic resistance (Nettelbladt & Sundblad, 1967). For example, the hydraulic conductivity of a hyaluronan concentration polarization layer extending 2 mm upstream from a membrane studied by Parker & Winlove (1984) had a hydraulic conductivity of 7 x 10-15 m4 s-' N-', using a bulk-phase grade III hyaluronan concentration of 15 g 1F' and filtration velocity -3 x 10-6 m s-' (3-5 times the estimated trans-synovial flow velocities in this study). The conductivity of the polarized layer was comparable with synovial interstitial permeability and was several orders of magnitude smaller than that of the bulk phase.…”

Section: Quantitative Aspects Of Hyaluronan Resistivitymentioning

confidence: 99%

Effect of intra‐articular hyaluronan on pressure‐flow relation across synovium in anaesthetized rabbits.

McDonald

Levick

1995

The Journal of Physiology

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1. Hyaluronan is the major polysaccharide of synovial fluid, responsible for its high viscosity.The effect of hyaluronan on fluid transport across the synovial lining of the joint was investigated. Rate of fluid absorption from the joint cavity (Qs) was measured at intraarticular pressures (PI) of up to 24 cmH20 in knees of anaesthetized rabbits, in the presence or absence of hyaluronan in intra-articular infusates. 2. Viscometry studies in vitro showed that the commercial hyaluronan used had a molecular weight of 549 000-774 000, a radius of gyration of 48-99 nm and a critical concentration for molecular overlap of 1P3 g lF'.3. With intra-articular Krebs solution (control) or subnormal, subcritical concentrations of hyaluronan (0-5 g F-), flow increased with pressure. Hyaluronan reduced the fluid escape rate by reducing slope dQ8/dPj by 32-64% relative to Krebs solution.4. At normal to high hyaluronan concentrations (3-6 g l-') and low pressures, hyaluronan again reduced slope dQO/dPI, by 39-64%. The reduction in slope was slight, however, when compared with the reduction in bulk fluidity (1/relative viscosity). Fluidity at high shear rates was reduced to 6 % of control values by 6 g 1-l hyaluronan. The effect on slope did not correlate significantly with the effect on fluidity.

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“…22 Briefly, in the fresh tissue the endothelium was badly damaged, the nuclei of the smooth muscle cells were very prominent and sandwiched between lamellae which stained for collagen but not elastin, and glycosaminoglycans (GAGs) were evident throughout the tissue. In the lysed tissue there were very few cell nuclei, a general reduction in G A G staining, and there was very little change in structure of the lamellae which stained slightly for elastin.…”

Section: Characterisation Of Tissue Preparationsmentioning

confidence: 99%

The Uptake of Ions and Neutral Solutes by the Artery and by Artery Wall Preparations

Winlove

Parker

Ewins

1988

Connective Tissue Research

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Radiotracer methods have been used to measure the distributions of inorganic ions in the aorta. Data on fresh tissue were generally consistent with previous measurements and showed UCa greater than UI greater than UNa. In order to relate these measurements to the macromolecular composition of the arterial wall, the effects of various interventions--cell lysis, autoclaving, and NaOH digestion--were investigated. Elastin was found to be the major determinant of ionic distribution coefficients, to possess a slight positive charge under physiological conditions, and to display a high affinity for Ca++. The distribution coefficients did not conform to the predictions of the Donnan theory of ionic equilibrium and the degree of nonideality, as measured by the fixed charge density and the ion selectivity coefficients, varied with ionic strength. Data are also presented on the distribution of neutral solutes of different molecular weights in the various arterial preparation, in order to provide a basis for comparison of the behaviour of charged solutes.

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The macromolecular basis of the hydraulic conductivity of the arterial hall

Cited by 22 publications

References 0 publications

Flow Through Interstitium and Other Fibrous Matrices

Flow Through Interstitium and Other Fibrous Matrices

Effect of intra‐articular hyaluronan on pressure‐flow relation across synovium in anaesthetized rabbits.

The Uptake of Ions and Neutral Solutes by the Artery and by Artery Wall Preparations

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