The maggot, the ethologist and the forensic entomologist: Sociality and thermoregulation in necrophagous larvae

Aubernon, Cindy; Hédouin, V.; Charabidzé, Damien

doi:10.1016/j.jare.2018.12.001

Cited by 22 publications

(28 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Live larvae of L. sericata are used for medical purposes due to their capability to remove necrotic tissue (Stadler, 2020) and to synthesize antimicrobial peptides against clinical Gram‐negative bacteria (Hirsch et al, 2019). Specimens are used in quality assurance in forensic science (Aubernon et al, 2019). In addition, the green bottle fly is an important ectoparasite of sheep, causing myiasis known as ‘blowfly strike’ in European countries (French et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Bacteria associated with Lucilia sericata larvae reared on fish wastes

Ulanova

Tikhonova

Кравченко

2020

Entomologia Exp Applicata

View full text Add to dashboard Cite

The green blowfly, Lucilia sericata (Meigen) (Diptera: Calliphoridae), is a cosmopolitan species of great medical, veterinary, and forensic importance. In addition, their larvae are among the most promising agents for the bioconversion of low‐quality biomass, such as organic waste, into sustainable and nutritionally valuable proteins for farmed fish and poultry. Despite the considerable medical and economic importance, the current literature provides limited information about microbiota associated with larvae. The present study aims to fill this knowledge gap. Freshly harvested L. sericata larvae (maggots) grown on fish wastes were investigated by conventional and molecular approaches to evaluate culturable microbial numbers and unculturable microbial diversity associated with the larval cuticle (external samples) and the internal body. In total 200 bacterial isolates were obtained; 46% of the strains originated from external samples and 58% originated from internal body samples produced extracellular protease enzymes, which may be involved in the digestion of proteins during larval feeding. In total 12 predominant bacteria with high proteolytic activity were further identified by morphological, physiological, biochemical, and molecular tools. Proteolytic bacteria in internal samples included Proteus, Providencia, Micrococcus, Deinococcus, whereas in external samples Providencia, Pseudomonas, and Acinetobacter were found. 16S rRNA clone library analysis revealed that the majority of internal bacteria (35%) were taxonomically assigned as Xanthomonadaceae (Schineria, Xylella, Ignnatzchineria), 28% Morganellaceae (Proteus, Providencia, Serratia), and 14% Enterobacteriaceae (Vagococcus, Serratia). Less abundant were bacteria of the genera Clostridium (3%), Erypelothrix (3%), and Oceanispherum (2%). This knowledge will be useful for biotechnological application of L. sericata.

show abstract

Section: Introductionmentioning

confidence: 99%

Bacteria associated with Lucilia sericata larvae reared on fish wastes

Ulanova

Tikhonova

Кравченко

2020

Entomologia Exp Applicata

View full text Add to dashboard Cite

show abstract

“…Indeed, highly efficient foraging aggregations that enhance survival rates compared to solitary foragers are documented in sawfly larvae (Ghent 1960), various species of caterpillars (Clark and Faeth 1997), where animals reared in larger groups displayed higher developmental rates and increased survival. This is also true for corpse-devouring necrophagous flies (Scanvion et al 2018;Aubernon et al 2019). Importantly, factors that provide trophic benefits can serve as tradeoffs in case of severe overcrowding (e.g., overly elevated temperature and proteotoxic stress caused by excessive tissue digestion, exemplified by Rivers et al 2011), implying a complex nonlinear pattern of relationship between group size (Cash et al 1993) and composition (Trowbridge 1991), food availability and distribution (Monaghan and Metcalfe 1985), presence or absence of predators (Turchin and Kareiva 1989) and individuals' investment into cooperative efforts (Valone 1989;Courchamp 1999;Giraldeau and Caraco 2018;Lindstedt et al 2018).…”

mentioning

confidence: 99%

Cooperative foraging during larval stage affects fitness in Drosophila

et al. 2020

View full text Add to dashboard Cite

Cooperative behavior can confer advantages to animals. This is especially true for cooperative foraging which provides fitness benefits through more efficient acquisition and consumption of food. While examples of group foraging have been widely described, the principles governing formation of such aggregations and rules that determine group membership remain poorly understood. Here, we take advantage of an experimental model system featuring cooperative foraging behavior in Drosophila. Under crowded conditions, fly larvae form coordinated digging groups (clusters), where individuals are linked together by sensory cues and group membership requires prior experience. However, fitness benefits of Drosophila larval clustering remain unknown. We demonstrate that animals raised in crowded conditions on food partially processed by other larvae experience a developmental delay presumably due to the decreased nutritional value of the substrate. Intriguingly, same conditions promote the formation of cooperative foraging clusters which further extends larval stage compared to nonclustering animals. Remarkably, this developmental retardation also results in a relative increase in wing size, serving an indicator of adult fitness. Thus, we find that the clustering-induced developmental delay is accompanied by fitness benefits. Therefore, cooperative foraging, while delaying development, may have evolved to give Drosophila larvae benefits when presented with competition for limited food resources.

show abstract

“…As the description of statistical models is not one of the objectives of this text, I take this opportunity instead to encourage readers to consult examples of the application of these models in recent forensic literature. For examples of GLMs/GLMMs, see [ 26 , 31 , 40 , 41 ]. For examples of GAMs/GAMMs, see [ 5 , 39 , 42 , 43 ].…”

Section: Possible Remedies To the Issues Associated With Successiomentioning

confidence: 99%

The Pitfalls in the Path of Probabilistic Inference in Forensic Entomology: A Review

Moreau

2021

Insects

View full text Add to dashboard Cite

To bridge the gap between experimentation and the court of law, studies in forensic entomology and other forensic sciences have to comply with a set of experimental rules to generate probabilistic inference of quality. These rules are illustrated with successional studies of insects on a decomposing substrate as the main example. The approaches that have been used in the scientific literature to solve the issues associated with successional data are then reviewed. Lastly, some advice to scientific editors, reviewers and academic supervisors is provided to prevent the publication and eventual use in court of forensic studies using poor research methods and abusing statistical procedures.

show abstract

The maggot, the ethologist and the forensic entomologist: Sociality and thermoregulation in necrophagous larvae

Abstract: Graphical abstract

Cited by 22 publications

References 41 publications

Bacteria associated with Lucilia sericata larvae reared on fish wastes

Bacteria associated with Lucilia sericata larvae reared on fish wastes

Cooperative foraging during larval stage affects fitness in Drosophila

The Pitfalls in the Path of Probabilistic Inference in Forensic Entomology: A Review

Contact Info

Product

Resources

About