The Major 20-kDa Polysaccharide ofStaphylococcus epidermidisExtracellular Slime and Its Antibodies as Powerful Agents for Detecting Antibodies in Blood Serum and Differentiating among Slime-Positive and -NegativeS. epidermidisand other Staphylococci Species

Karamanos, Nikos K.; Syrokou, Alexandra; Panagiotopoulou, H.S.; Anastassiou, Evangelos D.; Dimitracopoulos, G.

doi:10.1006/abbi.1997.0107

Cited by 30 publications

(12 citation statements)

References 30 publications

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“…and strain 35984 (RP62A) was obtained from the American Type Culture Collection. Both were clinical isolates, produce bio®lm, and have been well characterized [3, 15,18,20,21,22,26,35]. Bacterial stock cultures were maintained on tryptic soy agar (TSA; Difco, Becton Dickinson and Co., Sparks, Md.)…”

Section: Methodsmentioning

confidence: 99%

Interaction of Staphylococcus epidermidis with endothelial cells in vitro

Merkel

Scofield

2001

Med Microbiol Immunol

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Staphylococcus epidermidis is a leading cause of nosocomial bacteremia, yet virtually nothing is known about how this pathogen interacts with human endothelial cells. We present evidence here that two biofilm-producing strains of S. epidermidis adhere to two types of endothelial cell lines in vitro and that adherence is significantly increased after briefly heat-treating the bacteria at 40 degrees C in the presence of calcium. This mild heat treatment resulted in bacteria that were 5 to more than 20 times more adherent than untreated controls. While the adherence of bacteria in all phases of growth was increased after heat treatment, heat-treated late stationary phase cells were generally the most adherent. Electron microscopy demonstrated that S. epidermidis was internalized and appeared to exist free in the cytoplasm. Adherence to endothelium, should it occur in vivo during bacteremia, may be a virulence factor associated with this bacterium's pathogenesis.

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Section: Methodsmentioning

confidence: 99%

Interaction of Staphylococcus epidermidis with endothelial cells in vitro

Merkel

Scofield

2001

Med Microbiol Immunol

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“…Whole cell preparation of a S. aureus antigen proved to be the most sensitive assay (86%) when compared with six other antigens to identify endocarditis cases [72]. More recently, immunologic studies using ELISA against biofilm polysaccharide have shown promising results for the diagnosis of biofilm-mediated device infections [73][74][75][76]. An ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA) has been developed and tested in patients with staphylococcal late-onset synthetic vascular graft infections [77].…”

Section: Diagnosis Of Cardiac Device Infectionmentioning

confidence: 98%

Infections of electrophysiologic cardiac devices

Uslan¹

2008

Expert Review of Medical Devices

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Infections related to electrophysiologic cardiac device placement are increasing dramatically and are associated with adverse outcomes. With the US population aging and living longer, cardiac devices are utilized in an older population with significant comorbidities, making the threat from infectious complications significant. Electrophysiologic cardiac devices typically include an implanted pulse generator and an intravascular lead. Infection may occur at any component of the device, resulting in varied presentations. Bacteremia in cardiac device recipients may reflect primary generator pocket infection, or alternate sources of bacteremia may result in secondary hematogenous lead infection. This review focuses on the pathology, diagnosis, management and prevention of electrophysiologic cardiac device infections.

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“…Interestingly, Karamanos et al (26) suggested that the main component of the extracellular slime of S. epidermidis, including strain RP62A, was neither PIA nor teichoic acid but rather was a 20-kDa polysaccharide, the chemical structure of which has not been established.…”

mentioning

confidence: 99%

Extracellular Carbohydrate-Containing Polymers of a Model Biofilm-Producing Strain, Staphylococcus epidermidis RP62A

et al. 2005

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Staphylococcus aureus and coagulase-negative staphylococci, primarily Staphylococcus epidermidis, are recognized as a major cause of nosocomial infections associated with the use of implanted medical devices. It has been established that clinical isolates often produce a biofilm, which is involved in adherence to biomaterials and provides enhanced resistance of bacteria against host defenses and antibiotic treatments. It has been thought that the staphylococcal biofilm contains two polysaccharides, one responsible for primary cell adherence to biomaterials (polysaccharide/adhesin [PS/A]) and an antigen that mediates bacterial aggregation (polysaccharide intercellular adhesin [PIA]). In the present paper we present an improved procedure for preparation of PIA that conserves its labile substituents and avoids contamination with by-products. Based on structural analysis of the polysaccharide antigens and a thorough overview of the previously published data, we concluded that PIA from S. epidermidis is structurally identical to the recently described poly-␤-(136)-Nacetylglucosamine from PS/A-overproducing strain S. aureus MN8m. We also show that another carbohydratecontaining polymer, extracellular teichoic acid (EC TA), is an essential component of S. epidermidis RP62A biofilms. We demonstrate that the relative amounts of extracellular PIA and EC TA produced depend on the growth conditions. Moderate shaking or static culture in tryptic soy broth favors PIA production, while more EC TA is produced in brain heart infusion medium.

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The Major 20-kDa Polysaccharide ofStaphylococcus epidermidisExtracellular Slime and Its Antibodies as Powerful Agents for Detecting Antibodies in Blood Serum and Differentiating among Slime-Positive and -NegativeS. epidermidisand other Staphylococci Species

Cited by 30 publications

References 30 publications

Interaction of Staphylococcus epidermidis with endothelial cells in vitro

Interaction of Staphylococcus epidermidis with endothelial cells in vitro

Infections of electrophysiologic cardiac devices

Extracellular Carbohydrate-Containing Polymers of a Model Biofilm-Producing Strain, Staphylococcus epidermidis RP62A

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