The Major Histocompatibility Complex of Rhesus Monkeys XII: Cellular Typing for D Locus Antigens in Families¹

Abstract

Typing cells (TC) were used for the identification of D locus antigens in rhesus monkey families. The antigens defined by this cellular method always segregated with the other RhLA‐controlled markers except in one offspring with a proven recombination within the RhLA region; in that case, the D antigen was inherited in coupling with an antigen of the Ia1 locus (the analog of the human DR locus). With two exceptions, each TC or group of TC's was associated with one of the serologically defined Ia1 antigens. The two exceptions were associated with “blanks” of that series. Interestingly, two independent (mutually MLC responsive) TC groups appeared to be associated with the same Ia1 antigen 75*. Equally noteworthy was the observed “inclusion phenomenon” among cells of several TC groups, each associated with a single Ia1 antigen or blank of that series. In these cases, some of the TC's of the group were unidirectionally MLC‐responsive against other cells of the same group. Certain pitfalls of cellular typing are discussed; unexpected “false positive” results were attributed to prior immunization with products of non‐D loci which have been described recently (van Es & Balner 1978b).

Mixed Lymphocyte Reactivity in Chimpanzees. I. Some Technical and Genetic Aspects*

“…Thus, it seems that the D locus in the chimpanzee is very similar to the D locus in man and the rhesus monkey. The estimate of 10 alleles for the chimp's D locus is also in agreement with the degree of polymorphism known to exist for the D loci of man (Festenstein et al 1977, Keuning 1978) and rhesus monkey (van Es & Balner 1979). More recent MLC studies in which all members of the chimpanzee harems at this center (see Table 1) were used provided evidence for the existence of several "typing cells" homozygous for D locus antigens.…”

Mixed Lymphocyte Reactivity in Chimpanzees II. Family Studies and Identification of D Locus Antigens*

“…This might be an indication that the two TC's carry the same D antigen but differ for other antigens which influence MLC reactivity. Such patterns have also been observed with human and rhesus monkey TC's where groups of cells carrying the same D and DR antigen can sometimes be divided into two or more subgroups, so-called inclusions (Moller & Lindblom 1978, van Es & Balner 1979b, Suciu-Foca et al 1980, Jonker & Balner 1980b.…”