The Making of “Transgenic Spermatozoa”1

Célébi, Catherine; Guillaudeux, Thierry; Auvray, Pierrı̈ck; Vallet-Erdtmann, Virginie; Jégou, Bernard

doi:10.1095/biolreprod.102.009340

Cited by 48 publications

(36 citation statements)

References 75 publications

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“…Nevertheless this technique has a limited capability to incorporate the DNA into the host genome. In order to improve the DNA incorporation it is possible to expose the sperm to electrical field (electroporation), which has brought good results in cattle (Rieth et al 2000;Celebi et al 2003). …”

Section: State Of the Artmentioning

confidence: 99%

Animal transgenesis: state of the art and applications

et al. 2007

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There is a constant expectation for fast improvement of livestock production and human health care products. The advent of DNA recombinant technology and the possibility of gene transfer between organisms of distinct species, or even distinct phylogenic kingdoms, has opened a wide range of possibilities. Nowadays we can produce human insulin in bacteria or human coagulation factors in cattle milk. The recent advances in gene transfer, animal cloning, and assisted reproductive techniques have partly fulfilled the expectation in the field of livestock transgenesis. This paper reviews the recent advances and applications of transgenesis in livestock and their derivative products. At first, the state of art and the techniques that enhance the efficiency of livestock transgenesis are presented. The consequent reduction in the cost and time necessary to reach a final product has enabled the multiplication of transgenic prototypes around the world. We also analyze here some emerging applications of livestock transgenesis in the field of pharmacology, meat and dairy industry, xenotransplantation, and human disease modeling. Finally, some bioethical and commercial concerns raised by the transgenesis applications are discussed.

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Section: State Of the Artmentioning

confidence: 99%

Animal transgenesis: state of the art and applications

et al. 2007

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“…Sperm-mediated transgenesis involves different approaches: in vitro sperm-DNA incubation followed by in vitro fertilization or intracytoplasmic sperm injection (ICSI), and in vivo or in vitro germline gene transfer (Celebi et al, 2003).…”

Section: C) Sperm-mediated Transgenesismentioning

confidence: 99%

Transgenic Modifications of the Rat Genome

et al. 2005

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The laboratory rat (R. norvegicus) is a very important experimental animal in several fields of biomedical research. This review describes the various techniques that have been used to generate transgenic rats: classical DNA microinjection and more recently described techniques such as lentiviral vector-mediated DNA transfer into early embryos, sperm-mediated transgenesis, embryo cloning by nuclear transfer and germline mutagenesis. It will also cover techniques associated to transgenesis such as sperm cryopreservation, embryo freezing and determination of zygosity. The availability of several technologies allowing genetic manipulation in the rat coupled to genomic data will allow biomedical research to fully benefit from the rat as an experimental animal.

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“…In the last years, we have been interested to define the molecular mechanisms underlying sperm-mediated gene transfer for the generation of transgenic animals using sperm cells (see reviews by Spadafora, 1998;Chan et al, 2000;Celebi et al, 2003). In the course of our studies to characterize the mechanism of interaction between sperm cells and exogenous DNA molecules, we have found that these cells are endowed with an unexpected network of metabolic functions that are normally silent, yet are activated at fertilization (Spadafora, 1998).…”

Section: Endogenous Rt Activity Is Required For Mouse Early Embryonicmentioning

confidence: 99%

Endogenous reverse transcriptase: a mediator of cell proliferation and differentiation

Spadafora¹

2004

Cytogenet Genome Res

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Endogenous, non-telomeric Reverse Transcriptase (RT) is encoded by two classes of repeated genomic elements, retrotransposons and endogenous retroviruses, and is an essential component of the retrotransposition machinery of both types of elements. Expression of RT-coding genes is generally repressed in non-pathological, terminally differentiated cells, but is active in early embryos, germ cells, embryo and tumor tissues, all of which have a high proliferative potential. To clarify whether reverse transcription is functionally implicated in control of cell growth, differentiation and in embryogenesis, recent experiments have been undertaken to inactivate the endogenous RT activity. RT was inhibited in normal and transformed cell lines by exposure to nevirapine, a non-nucleosidic RT inhibitor. The endogenous RT was also blocked in murine embryos by microinjection of an anti-RT antibody. Both experimental approaches yielded a dramatic inhibition of proliferation. Murine embryos arrested at pre-implantation stages. Transformed cell lines underwent a significant reduction in the rate of cell growth, concomitant with the induction of differentiation. In addition, RT inhibition induced an extensive reprogramming of the gene expression profile both in cultured cell lines and in preimplantation embryos. From these studies, endogenous RT begins to emerge as a key function with a driving role in normal and pathological developmental processes.

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The Making of “Transgenic Spermatozoa”1

Cited by 48 publications

References 75 publications

Animal transgenesis: state of the art and applications

Animal transgenesis: state of the art and applications

Transgenic Modifications of the Rat Genome

Endogenous reverse transcriptase: a mediator of cell proliferation and differentiation

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