The MAP kinase AflSlt2 modulates aflatoxin biosynthesis and peanut infection in the fungus Aspergillus flavus

Zhang, Feng; Geng, Longpo; Deng, Jili; Huang, Luhua; Zhong, Hong; Xin, Sijie; Fasoyin, Opemipo Esther; Wang, Shihua

doi:10.1016/j.ijfoodmicro.2020.108576

Cited by 20 publications

(28 citation statements)

References 63 publications

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“…Prior studies in our laboratory revealed that both the Hog1‐MAPK and Slt2‐MAPK pathways are critical for the regulation of development, AF biosynthesis and pathogenicity in A . flavus (Tumukunde et al ., 2019; Zhang et al ., 2020). However, the functions of Fus3‐MAPK pathways in A. flavus are still poorly understood.…”

Section: Resultsmentioning

confidence: 99%

“…7), and Msg5 was identified as a main negative regulator of these phosphatases, therefore, we investigated the relationship between Msg5 and MAPKs (Slt2, Fus3 and Hog1) in A. flavus. The MAPKs Hog1 and Slt2 have already been shown to be involved in the regulation of development, secondary metabolism, stresses response and pathogenicity (Tumukunde et al, 2019;Zhang et al, 2020), but another substrate of MAPK phosphatase-Fus3 remained still uncharacterized in A. flavus. Therefore, we further investigated the function of Fus3 in A. flavus.…”

Section: Discussionmentioning

confidence: 99%

“…flavus . The MAPKs Hog1 and Slt2 have already been shown to be involved in the regulation of development, secondary metabolism, stresses response and pathogenicity (Tumukunde et al ., 2019; Zhang et al ., 2020), but another substrate of MAPK phosphatase‐Fus3 remained still uncharacterized in A. flavus . Therefore, we further investigated the function of Fus3 in A .…”

Section: Discussionmentioning

confidence: 99%

“…In filamentous fungi, the three conserved MAPK pathways (Fus3-MAPK, Slt2-MAPK and Hog1-MAPK) are involved in various cellular processes, including mating, cell cycle, cell wall integrity, response to stresses and pathogenicity (Martinez-Soto and Ruiz-Herrera, 2017). Prior studies in our laboratory revealed that both the Hog1-MAPK and Slt2-MAPK pathways are critical for the regulation of development, AF biosynthesis and pathogenicity in A. flavus (Tumukunde et al, 2019;Zhang et al, 2020). However, the functions of Fus3-MAPK pathways in A. flavus are still poorly understood.…”

Section: Mapk-related Tyrosine Phosphatases Regulate Phosphorylation mentioning

confidence: 98%

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MAPK pathway‐related tyrosine phosphatases regulate development, secondary metabolism and pathogenicity in fungus Aspergillus flavus

Guang

Cao

et al. 2020

Environmental Microbiology

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Mitogen-activated protein kinase (MAPK) cascades are highly conserved in eukaryotic cells and are known to play crucial roles in the regulation of various cellular processes. However, compared with kinase-mediated phosphorylation, dephosphorylation catalysed by phosphatases has not been well characterized in filamentous fungi. In this study, we identified five MAPK pathway-related phosphatases (Msg5, Yvh1, Ptp1, Ptp2 and Oca2) and characterized their functions in Aspergillus flavus, which produces aflatoxin B 1 (AFB 1), one of the most toxic and carcinogenic secondary metabolites. These five phosphatases were identified as negative regulators of MAPK (Slt2, Fus3 and Hog1) pathways. Deletion of Msg5 and Yvh1 resulted in significant defects in conidiation, sclerotia formation, aflatoxin production and crop infection. Additionally, double knockout mutants (ΔMsg5/ΔPtp1, ΔMsg5/ΔPtp2 and ΔMsg5/ΔOca2) displayed similar defects to those observed in the ΔMsg5 single mutant, indicating that Msg5 plays a major role in the regulation of development and pathogenicity in A. flavus. Importantly, we found that the active site at C439 is essential for the function of the Msg5 phosphatase. Furthermore, the MAP kinase Fus3 was found to be involved in the regulation of development, aflatoxin biosynthesis and pathogenicity, and its conserved phosphorylation residues (Thr and Tyr) were critical for the full range of its functions in A. flavus. Overall, our results reveal that MAPK related tyrosine phosphatases play important roles in the regulation of development, secondary metabolism and pathogenicity in A. flavus, and could be developed as potential targets for preventing damage caused by this fungal pathogen.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Mapk-related Tyrosine Phosphatases Regulate Phosphorylation mentioning

confidence: 98%

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MAPK pathway‐related tyrosine phosphatases regulate development, secondary metabolism and pathogenicity in fungus Aspergillus flavus

Guang

Cao

et al. 2020

Environmental Microbiology

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“…Five main MAPK signalling pathways have been identified and characterized in Saccharomyces cerevisiae , including the high osmolarity glycerol (HOG) pathway, the mating response pathway, filamentous and invasive growth (FIG) pathway, cell wall integrity (CWI) pathway and pheromone pathway (Saito and Posas, 2012). But in A. flavus , only three central MAP kinases orthologous to yeast, including SakA (Hog1), Slt2 (MPKA) and Fus3 (MPKB), have been described (Tumukunde et al ., 2019; Zhang et al ., 2020; Frawley et al ., 2020). These MAP kinases have distinct functions in asexual sporulation, sclerotia formation and aflatoxin production.…”

Section: Introductionmentioning

confidence: 99%

The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus

Qin

Zhao

et al. 2020

Microbial Biotechnology

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As a pathogenic fungus, Aspergillus flavus can produce carcinogenic aflatoxins (AFs), which poses a great threat to crops and animals. Msb2, the signalling mucin protein, is a part of mitogen-activated protein kinase (MAPK) pathway which contributes to a range of physiological processes. In this study, the roles of membrane mucin Msb2 were explored in A. flavus by the application of gene disruption. The deletion of msb2 gene (Dmsb2) caused defects in vegetative growth, sporulation and sclerotia formation when compared to WT and complement strain (Dmsb2 C) in A. flavus. Using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis, it was found that deletion of msb2 down-regulated aflatoxin B 1 (AFB 1) synthesis and decreased the infection capacity of A. flavus. Consistently, Msb2 responds to cell wall stress and osmotic stress by positively regulating the phosphorylation of MAP kinase. Notably, Dmsb2 mutant exhibited cell wall defect, and it was more sensitive to inhibitor caspofungin when compared to WT and Dmsb2 C. Taking together, these results revealed that Msb2 plays key roles in morphological development process, stresses adaptation, secondary metabolism and pathogenicity in fungus A. flavus.

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Aflatoxin Biodetoxification Strategies Based on Postbiotics

Khani,

Noorkhajavi,

Soleiman

et al. 2024

Probiotics & Antimicro. Prot.

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The MAP kinase AflSlt2 modulates aflatoxin biosynthesis and peanut infection in the fungus Aspergillus flavus

Cited by 20 publications

References 63 publications

MAPK pathway‐related tyrosine phosphatases regulate development, secondary metabolism and pathogenicity in fungus Aspergillus flavus

MAPK pathway‐related tyrosine phosphatases regulate development, secondary metabolism and pathogenicity in fungus Aspergillus flavus

The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus

Aflatoxin Biodetoxification Strategies Based on Postbiotics

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